Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

EADT1 gene capable of improving drought resistance of rice in growth period, coding sequence and application

A drought resistance and rice technology, applied in the fields of genetic engineering and molecular biology, can solve the problem of no transfer of lipids, and achieve great application value, increase growth, and improve plant stress resistance.

Inactive Publication Date: 2013-10-16
FUDAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular weight of LTP2 is usually around 7 Da (Carvalho & Gomes, 2007); there is also a class of LTP-like proteins, although they are similar to LTP in terms of primary structure and disulfide bond arrangement, but there are excessive Many charged amino acids, no lipid transfer activity in vitro, only found in onion seeds (Cammue et al, 1995)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • EADT1 gene capable of improving drought resistance of rice in growth period, coding sequence and application
  • EADT1 gene capable of improving drought resistance of rice in growth period, coding sequence and application
  • EADT1 gene capable of improving drought resistance of rice in growth period, coding sequence and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 rice under drought treatment EADT1 Identification of Gene Expression Levels During Reproductive Development of Rice Variety Nipponbare

[0043] 1. Coercion treatment

[0044] After the rice Nipponbare seeds germinate, move them to potting soil for cultivation, stop watering at the booting stage, and carry out drought treatment. When the soil moisture drops to about 30%, keep it for a week, and take different sizes (2~3mm, 3~4mm, Rice flowers of 4~5mm, 5~7mm) were collected and numbered respectively, and the rice under normal growth conditions was used as a control, and three repetitions were made.

[0045] 2. Gene chip and fluorescent quantitative PCR analysis of gene expression under drought conditions

[0046] Analysis of gene expression in rice under drought conditions using Agilent's Rice Microarray. Comparing different periods of flower development under drought versus normal conditions EADT1 Gene expression in rice variety Nippon Haruka. The resul...

Embodiment 2

[0047] Example 2 rice EADT1 Cloning of gene cDNA fragments

[0048] 1. Extraction of RNA: take drought-treated rice flowers, freeze them with liquid nitrogen in a mortar, grind them into powder, add 1 ml of TRIzol reagent (Promega) into an EP tube, oscillate fully, and place at room temperature for 5 minutes. After centrifugation at 12000 rpm for 10 min at 4°C, transfer the supernatant to a new EP tube, add 200 μl of chloroform to make an emulsion, let stand at room temperature for 5 minutes, centrifuge at 12000 rpm for 10 min, and transfer the supernatant to a new EP tube, Add twice the volume of isopropanol to precipitate RNA, centrifuge, dissolve the precipitate in water after drying, and then measure the RNA content on a spectrophotometer.

[0049] 2. Reverse transcription: according to TaKaRa's PrimeScript TM Reverse Transcriptase instructions for operation: add 5 μl (5 μg) of rice total RNA to the centrifuge tube, Oligo(dT) 15 Add 1 μl of Primer (50 μmol / L), 1 μl ...

Embodiment 3

[0053] Example 3 rice EADT1 sequence information and homology analysis

[0054] EADT1 The length of the coding region of the gene is 348bp, and its sequence is shown in SEQ ID NO.2. The amino acid sequence of rice EADT1 was deduced according to the full-length cDNA, with a total of 115 amino acid residues, a molecular weight of 12223.1 Daltons, and an isoelectric point (pI) of 5.67. The sequence is shown in SEQ NO.3. Bioinformatics analysis showed that the protein was an unstable hydrophobic protein.

[0055] Perform BLAST comparison of this protein in GenBank to obtain the protein sequence homologous to this protein, use Clustal X software for comparison, and then use MEGA5 software to construct a phylogenetic tree according to the proximity method ( figure 2 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical fields of molecular biology and genomic engineering, in particular to an EADT1 gene capable of improving drought resistance of rice in a growth period, a coding sequence and application. The invention relates to a coding sequence of a plant lipid transfer protein gene EADT1 expressed in the rice, and the coding sequence mainly has effects of improving the drought resistance of the rice in the growth period (including but not limited to the growth period) and improving the maturing rate of the rice. The invention also discloses a recombinant vector comprising the gene sequence, and a transgenic plant transformed by the vector. The gene is subjected to transformation expression in a plant, the maturing rate of the transgenic rice under drought stress can be improved, and the yield loss of crops in adverse situations such as drought is reduced.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, in particular to a lipid transfer protein expressed in rice EADT1 Cloning of genes, construction of transgenic vectors and application in improving drought tolerance and seed setting rate of rice. Background technique [0002] Rice is the most important food crop in my country, and it is also the crop that uses the most water in agricultural production. Drought has seriously affected the yield of rice. How to reduce the dependence of rice on water and make it have the characteristics of water saving and drought resistance is of great significance to my country's food, water and ecological security. An important determinant of yield is the seed setting rate of rice at the flowering stage. Rice has different tolerance to drought at different developmental stages (Saini & Lalonde, 1998; Yang & Zhang, 2006). When there is a lack of water in the vegetative period,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00C12Q1/68
Inventor 马红葛晓春郭长奎
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com