Method for preparing medical titanium alloy with bionic multilayered structure interface

A multi-layer structure, titanium alloy technology, applied in chemical instruments and methods, coatings, medical science, etc., can solve the problem of not providing a suitable extracellular microenvironment for cells, difficult to control the thickness of hydroxyapatite, difficult to control hydroxyphosphate limestone morphology and other issues, to achieve good biocompatibility and osseointegration, affinity, strong activity, and low cost.

Active Publication Date: 2014-09-03
CHONGQING UNIV
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Problems solved by technology

[0004] At present, there are some problems in this kind of research: first, covering the surface of titanium with hydroxyapatite coating by physical and chemical methods can improve the biocompatibility of titanium materials, but it is difficult to control the form of hydroxyapatite by such methods. However, studies have shown that hydroxyapatite in natural bone is needle-shaped. On the other hand, its harsh physical and chemical conditions are not conducive to the introduction of bioactive molecules, and cannot provide cells with a suitable extracellular microenvironment. At the same time, such It is also difficult to control the thickness of hydroxyapatite; second, mix hydroxyapatite nanoparticles with bioactive molecules to prepare scaffold materials containing hydroxyapatite particles, which can provide a good microenvironment for cells , to promote the function of cells, but this kind of structure ignores the nanoscale structure unique to natural bone, which is not conducive to the induction of the formation of ring bone plate in the later stage

Method used

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  • Method for preparing medical titanium alloy with bionic multilayered structure interface
  • Method for preparing medical titanium alloy with bionic multilayered structure interface
  • Method for preparing medical titanium alloy with bionic multilayered structure interface

Examples

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Embodiment 1

[0024]Example 1. Construction of a biomimetic multilayer structure on the surface of a titanium alloy that promotes osteoblast differentiation

[0025] 1) Preparation of xonotlite nanowires: Calcium nitrate and sodium silicate were prepared into 0.5mol / L solutions respectively; secondly, calcium nitrate solution was added dropwise to sodium silicate solution, and magnetically stirred for 2 hours to form a white suspension Then the white suspension was transferred to a high-pressure reactor lined with tetrafluoroethylene, heated in an oil bath, and reacted at 200°C for 24 hours; the product was washed three times with absolute ethanol and distilled water, filtered, Freeze-dried overnight to obtain xonotlite nanowires.

[0026] 2) Preparation of hydroxyapatite nanowires: Trisodium phosphate was formulated into a solution with a concentration of 0.2 mol / L; 1 g of xonotlite precursor was added to 85 mL of trisodium phosphate solution, and magnetically stirred for 2 hours to form a...

experiment example 1

[0036] Experimental example 1. Cytocompatibility of titanium alloy after layered multilayer structure modification

[0037] This study investigated the cellular activity of osteoblasts on the surface of titanium alloys with multilayer structures.

[0038] The cytocompatibility of different modified titanium surfaces was characterized by MTT. When the primary osteoblasts were cultured to the third passage, 1×10 4 The density of the cells / wells is terminated on the surface of different modified titanium alloy sheets and on TCPS, cultured at 37°C and 5% CO2. After 4 days and 7 days, the culture medium was aspirated and discarded, and 1 mL of fresh serum-free medium and 100 μL of MTT solution (5 mg / mL) were added, and the culture was continued for 4 h. Aspirate the culture solution, add 500 μL dimethyl sulfoxide (DMSO), and when the crystals are completely dissolved, measure the absorbance value of each well at 490 nm.

[0039] MTT, tetrazolium salt, is a yellow dye that can en...

experiment example 2

[0040] Experimental example 2. Alkaline phosphatase activity of osteoblasts on titanium alloy surface after layered multilayer structure modification

[0041] The third generation osteoblasts were divided into 2×10 4 The density of cell / well was seeded on the surface of each titanium sheet and TCPS. After culturing for 4 days and 7 days respectively, the cells were lysed with 1% TritonX-100 to obtain a cell lysate. With p-nitrophenol as the catalytic substrate of alkaline phosphatase, under the action of alkaline phosphatase, the reaction produces a yellow water-soluble product, the absorbance value is measured at a wavelength of 405nm, and the base of the corresponding experimental group is obtained according to the standard curve. Sexual phosphatase activity. From image 3 It can be found that after 4 days of culture, the Ti / LbL / HA group showed higher ALP activity than the Ti and Ti / LbL groups, and there was a very significant difference, while there was no difference bet...

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Abstract

The invention discloses a method for preparing a medical titanium alloy with a bionic multilayered structure interface. The method comprises the following steps: preparing a hydroxyapatite nanowire through a precursor hydrothermal replacement method, and taking the nanowire as an intercalation material for constructing a simulated natural bone laminar film; and assembling the hydroxyapatite nanowire into a gelatin/chitosan multilayer film by utilizing a layer-by-layer self-assembly technology (a spin-coating method), and thus constructing a film for simulating the natural bone nano layered structure from the bionic angle on the surface of a titanium material. Therefore, the biological function of osteoblast is controlled, and the biocompatibility and bone integration of a titanium alloy transplant are improved.

Description

technical field [0001] The invention belongs to the field of biomedical materials and relates to a preparation method of a functional titanium alloy interface. Background technique [0002] Titanium and titanium alloy implants are widely used in surgical operations such as bone repair because of their excellent mechanical properties and good biocompatibility. Then, after the titanium graft is implanted in the body, it combines with the bone in a mechanically integrated manner. This passive method will cause inflammation, displacement, and eventually lead to graft failure. In order to solve this problem, surface modification of titanium to improve its osseointegration has become an important content in the research field of medical materials. [0003] The study showed that the calcified layer of natural bone consists of osteons, a layered structure called a ring bone plate assembled (mainly) from hydroxyapatite nanoparticles and collagen molecules. This layered structure pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/32A61L27/40C01B25/32
Inventor 蔡开勇奚兴凤杨维虎
Owner CHONGQING UNIV
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