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A biological scaffold material for periodontal tissue and its preparation method

A bio-scaffold and periodontal tissue technology, which is applied in the direction of introducing foreign genetic material, pharmaceutical formulations, textiles and papermaking using carriers, achieving the effects of fast degradation, excellent mechanical properties and easy operation.

Inactive Publication Date: 2015-10-14
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the regeneration and reconstruction of bone tissue is the result of the joint action of multiple genes and factors, and the growth factor expressed by a single gene cannot complete the process of bone regeneration well.

Method used

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  • A biological scaffold material for periodontal tissue and its preparation method
  • A biological scaffold material for periodontal tissue and its preparation method
  • A biological scaffold material for periodontal tissue and its preparation method

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preparation example Construction

[0036] A preparation method for a bio-scaffold material for periodontal tissue (dual gene activation matrix applied to tissue engineering), comprising the following steps:

[0037] 1. Dissolve the two gene plasmids in the gene carrier G5-PAMAM-D solution respectively, and let the obtained mixture stand for 30 minutes to form two kinds of G5-PAMAM-D gene plasmid carrier complex nanospheres;

[0038] 2. Dissolving the two gene plasmid carrier complex nano-microspheres in a degradable organic polymer solvent with a mass concentration of 10% to 15% respectively to prepare a shell solution and a core solution;

[0039] 3. The shell solution and the core solution are coaxially electrospun with needles of different inner diameters arranged concentrically, the inner diameter of the core layer is 0.06-1mm, and the inner diameter of the shell layer is 0.2-2mm. The flow rate of the core layer solution is 0.1-1ml / h, and the flow rate of the shell layer solution is 0.5-15ml / h; the distance...

Embodiment 1

[0042] A preparation method for a biological scaffold material for periodontal tissue, comprising the following steps:

[0043] 1) Preparation of plasmid vector composite nanospheres:

[0044]Under the condition of gentle vortex stirring, quickly add 1 mL of G5-PAMAM-D aqueous solution with a mass concentration of 30% to the 100 μL PBS solution of pIRES2-EGFP-hBMP2 and pDsRed2-N1-hOPG plasmids, mix well, and let stand for 30 minutes. Obtain pIRES2-EGFP-hBMP2-G5-PAMAM-D composite nanospheres and pDsRed2-N1-hOPG-G5-PAMAM-D composite nanospheres;

[0045] 2) Prepare shell solution and core solution:

[0046] Prepare shell solution: dissolve polycaprolactone (PCL, molecular weight 80,000, viscosity coefficient 0.8dl / g) in dichloromethane / N,N-dimethylformamide mixed solvent (v / v=8:2 ), was prepared into a solution with a mass concentration of 10%; the pIRES2-EGFP-hBMP2-G5-PAMAM-D composite nanospheres prepared in step 1) were added into a PCL solution with a mass concentration of...

Embodiment 2

[0051] A preparation method for a biological scaffold material for periodontal tissue, comprising the following steps:

[0052] 1) Preparation of plasmid vector composite nanospheres:

[0053] Under gentle vortex stirring conditions, quickly add 500 μL of 20% G5-PAMAM-D aqueous solution to the 100 μL PBS solution of pIRES2-EGFP-hBMP2 and pDsRed2-N1-hOPG plasmids, mix well, and let stand for 30 minutes. Obtain pIRES2-EGFP-hBMP2-G5-PAMAM-D composite nanospheres and pDsRed2-N1-hOPG-G5-PAMAM-D composite nanospheres;

[0054] 2) Prepare shell solution and core solution:

[0055] Prepare shell solution: dissolve polycaprolactone in dichloromethane / N,N-dimethylformamide mixed solvent (v / v=8:2) to prepare a solution with a mass concentration of 15% (w / w) ; The pIRES2-EGFP-hBMP2-G5-PAMAM-D composite nanosphere prepared in step 1) is added to the polycaprolactone solution with a mass concentration of 15%, and mixed to make a shell solution;

[0056] Prepare the core layer solution: d...

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Abstract

The invention discloses a biological scaffold material for periodontium and a preparation method of the biological scaffold material, which belongs to the technical field of biologic tissue engineering scaffolds. The biological scaffold material is prepared from a shell solution and a core solution by using a coaxial electrostatic spinning method, wherein the shell solution and the core solution are prepared by respectively dissolving and subsequently uniformly mixing carrier compound nano microspheres containing one gene plasmid into a degradable organic macromolecule solvent. The biological scaffold material which is provided with a core-shell structure and is prepared by using the coaxial electrostatic spinning method is simple in process, easy to operate, low in cost and can be applied to non-virus gene therapy in tissue engineering mediation.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering brackets, and in particular relates to a biological bracket material for periodontal tissue and a preparation method thereof. Background technique [0002] Periodontal disease refers to diseases that occur in the tooth supporting tissue (periodontal tissue), including gingival disease involving only gingival tissue and periodontitis involving deep periodontal tissue (periodontal ligament, alveolar bone, cementum). category. Periodontal disease is a common oral disease, one of the main causes of adult tooth loss, and a major oral disease that endangers human teeth and general health. Existing treatment methods are only maintained at basic treatment. With the rapid development of tissue engineering technology, periodontal tissue engineering technology has brought new hope for periodontal disease treatment and periodontal defect repair, and has become a hot spot in periodontal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00C12N15/63A61K47/34A61L27/54A61L27/50D01D5/34
Inventor 贾骏徐宏宇贾列妮郭秋云董瑜
Owner FOURTH MILITARY MEDICAL UNIVERSITY