Method for increasing butanol-acetone ratio and butanol yield of clostridia ABE fermentation

A technology of Clostridium acetobutylicum and butanol fermentation, which is applied in the field of ABE fermentation, can solve the problems of increasing difficulty, butanol production does not reach the inhibitory concentration of Clostridium acetobutylicum, and the ratio of butanol/acetone does not increase significantly, so as to improve the comprehensive performance , simple implementation, effective use of waste water
CN104372033AActive Publication Date: 2015-02-25JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2015-02-25

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Abstract

The invention discloses a method for increasing the butanol-acetone ratio and the butanol yield of clostridia ABE fermentation, and belongs to the technical field of biology. According to the method, corn flour is taken as a fermentation raw material, and a clostridium-saccharomyces cerevisiae mixed culture system is formed by appropriately adding an active saccharomyces cerevisiae and a butyric acid solution at the solvent production stage of the clostridia. The saccharomyces cerevisiae is capable of secreting various amino acids under a strict anaerobic condition and in a severe saccharomyces cerevisiae growth environment, wherein aromatic amino acids and aspartic amino acids all advantageous for butanol synthesis are greatly accumulated. On the basis, the small amount of added butyric acid is further capable of weakening the metabolism intensity of the closed loop of butyric acid. Under the dual combined action, the butanol-acetone ratio and the final butanol concentration of the ABE fermentation can be greatly improved under the premise of unchanged or slightly improved total solvent production intensity. The two indexes, namely butanol-acetone ratio and butanol concentration of the method are at the highest level in the related study reports of the batch fermentation production of butanol by use of wild clostridia and have important guiding significance for the ABE fermentation.
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Description

technical field

[0001] The invention relates to a method for improving the butanol / acetone ratio and butanol yield of ABE fermentation, especially an ABE fermentation method for improving the butanol / acetone ratio and butanol yield through mixed culture of Clostridium acetobutylicum and Saccharomyces cerevisiae, belonging to biotechnology field. Background technique

[0002] Butanol is an important platform compound, which is widely used in chemical industry, plastics, medicine, organic synthesis, printing and dyeing and other industries. At the same time, bio-butanol is also a promising, clean and renewable new liquid fuel that can be used as a substitute for fossil fuels. The development of biofuels has become an important measure for countries all over the world to deal with the energy crisis, reduce greenhouse gas emissions, and improve the climate environment. Butanol fermentation is generally divided into two stages: the first stage is the acidogenic stage, butyric a...

Claims

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