A Collagen Purification and Preservation Method Based on Magnetic Recyclable Nano Antibacterial Agent
A nano-antibacterial agent and magnetic nano-particle technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and chemicals for biological control, etc., can solve the problems of inability to separate, recover, and affect the biocompatibility of collagen. , to achieve the effect of ensuring comprehensiveness and thoroughness, strong antibacterial activity and high antibacterial efficiency
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Embodiment 1
[0030] (1) Preparation of magnetic nanoparticles with double bonds on the surface: 0.6 parts of nano-Fe with a particle size of 10nm 3 o 4 and 0.4 parts of γ-Fe with a particle size of 50nm 2 o 3 Disperse in 150 parts of an aqueous solution with a volume fraction of 90% ethanol, then add 2 parts of vinyltrimethoxysilane dropwise, and stir at room temperature for 20 minutes; then add 4 parts of glacial acetic acid dropwise, while raising the temperature to 40°C for 4 hours , the obtained product is separated by a magnet, washed repeatedly with absolute ethanol, and dried to a constant weight to obtain magnetic nanoparticles with double bonds on the surface;
[0031] (2) Preparation of vinylated ciprofloxacin: Mix 10 parts of ciprofloxacin, 4 parts of pyridine and 300 parts of dichloromethane evenly, stir at 0°C for 30 minutes, then add dropwise under continuous stirring and inert gas protection 4 parts of acryloyl chloride, after the dropwise addition, the temperature was ra...
Embodiment 2
[0036] (1) Preparation of magnetic nanoparticles with double bonds on the surface: 3 parts of nano-Fe with a particle size of 20nm 3 o 4 Disperse in 170 parts of an aqueous solution with a volume fraction of 90% ethanol, then add 6 parts of methacryloxypropyltrimethoxysilane dropwise, and stir at room temperature for 30 minutes; then add 4 parts of ammonia water and 4 parts of sodium carbonate dropwise At the same time, the temperature was raised to 50 ° C for 5 hours, and the obtained product was separated by a magnet, washed repeatedly with absolute ethanol, and dried to a constant weight to obtain a magnetic nano-Fe with double bonds on the surface. 3 o 4 ;
[0037] (2) Preparation of vinylated norfloxacin: Mix 13 parts of norfloxacin, 5 parts of triethylamine and 400 parts of dichloromethane evenly, stir at 2°C for 40 minutes, and then under continuous stirring and inert gas protection Add 5 parts of acryloyl chloride dropwise, and after the dropwise addition, raise the...
Embodiment 3
[0042] (1) Preparation of magnetic nanoparticles with double bonds on the surface: 8 parts of γ-Fe with a particle size of 30nm 2 o 3 Dispersed in 180 parts of ethanol volume fraction of 90% aqueous solution, then dropwise added 6 parts of vinyl tri( β -Methoxyethoxy) silane, and stirred at room temperature for 20 minutes; then 8 parts of potassium carbonate and 4 parts of potassium hydroxide were added dropwise, and the temperature was raised to 40°C for 4 hours, and the obtained product was separated with a magnet and used without After repeated washing with water and ethanol, and drying to constant weight, γ-Fe with double bonds on the surface can be obtained. 2 o 3 ;
[0043] (2) Preparation of vinylated enoxacin: 16 parts of enoxacin, AlCl 3 Mix 7 parts and 450 parts of tetrahydrofuran evenly, stir at 3°C for 50 minutes, then add 7 parts of 4-pentenoyl chloride dropwise under continuous stirring and inert gas protection, and raise the temperature to 25°C to react fo...
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