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Preparation method of selenium-rich protein peptide leaf fertilizer

A technology of selenium-enriched protein and foliar fertilizer, applied in fertilization device, fertilizer mixture, application and other directions, can solve the problems of low element utilization rate, unstable effect, single nutrient composition, etc., to improve the quality and added value of crops. The effect of high, high protein content

Inactive Publication Date: 2016-03-09
CHONGQING SANLINGSAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, most of the foliar fertilizers produced in China have low effective content and single function. Most of them are to supplement trace elements for crops and have single nutritional components.
The Chinese patent with the patent number CN101891520 disclosed an amino acid foliar fertilizer on August 29, 2012, which is prepared from the following materials in terms of mass percentage: 30%-50% plant amino acids, 1%-5% compound nitrophenol Sodium, 15%-25% urea, 5%-10% ferrous sulfate, 5%-10% borax, 3%-5% copper sulfate, 0.5%-1% ammonium molybdate, 1%-2% manganese sulfate, 4%-8% zinc sulfate, 10%-20% organic silicon and the balance of water, the foliar fertilizer stimulates the activity of enzymes in crops, improves the photosynthetic efficiency of crops, accelerates the absorption and operation of internal nutrients, and increases the content of chlorophyll , increase the accumulation of stem matter and sugar content, improve crop quality, enhance crop resistance to drought, disease resistance, stress resistance and immunity, etc., all have obvious effects, but there are also deficiencies, for example, a single mixture of multiple elements to make a concentrated solution , mixed with water, the element utilization rate is not high, the effect is not stable enough; after absorption, the transportation efficiency in the crop is low, etc.
There are few reports on the application of porcine blood protein peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing selenoprotein peptide foliar fertilizer, the steps are as follows: (1) Preparation of protein peptide enzymatic hydrolysis solution: a) collect fresh pig blood, anticoagulate with 5g / L sodium citrate, and heat at 4°C, 8000r Centrifuge for 10 minutes per minute, collect the lower layer of sediment to obtain red blood cells; add the same volume of normal saline to the red blood cells to wash and centrifuge twice, then add 2 times the volume of distilled water, ultrasonic cell disruption assists hemolysis for 20 minutes, and then centrifuge at 4°C and 4000r / min After 10 minutes, the hemoglobin solution was obtained, and stored at 4°C for later use; b) Take the hemoglobin solution, add an appropriate amount of trypsin to enzymolyze it, stir, and maintain the pH of the system with 2mol / L NaoH solution at 45-50°C for 6-10 hours; The reaction product is rapidly heated to 85°C and maintained at this temperature for 10-15 minutes, then rapidly cooled to bel...

Embodiment 2

[0027] A preparation method of selenoprotein peptide foliar fertilizer, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as in Example 1; (2) Carboxymethyl chitosan, sodium selenite and citric acid are prepared according to the The mass ratio is 100:1:15, and the nano-selenium solution is prepared in the water phase, the temperature is controlled at 60 °C, and the mass fraction of chitosan in the solution is 5×10 -4 , the concentration of selenium compound is 3mmol / L, and the concentration of citric acid is 7g / L; (3) Add the nano-selenium solution to the enzymatic hydrolysis solution at a temperature of 55°C for 2 hours to obtain a liquid; (4) Add the The finished product is obtained by concentrating the liquid and spray drying, wherein the air inlet temperature is 220°C and the air outlet temperature is 90°C during spray drying.

Embodiment 3

[0029] A preparation method of selenoprotein peptide foliar fertilizer, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as in Example 1; (2) Carboxymethyl chitosan, sodium selenite and vitamin C are prepared according to the The mass ratio is 90:1:12, and the nano-selenium solution is prepared in the water phase, and the temperature is controlled at 65°C for 2 hours, and then the ultrasonic wave is 100W, 15kHz for 30 minutes, and the treatment is 6 minutes and 7 minutes. The quality of chitosan in the solution The fraction is 5×10-4, the concentration of selenium compound is 3mmol / L, and the concentration of citric acid is 6g / L; (3) Add the nano-selenium solution into the enzymatic hydrolysis solution at a temperature of 60°C, and chelate for 1.5h to obtain into a liquid; (4) concentrating the liquid and spray drying to obtain a finished product, wherein the temperature of the air inlet during spray drying is 230°C and ...

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Abstract

The invention relates to a preparation method of a selenium-rich protein peptide leaf fertilizer. The preparation method comprises the following steps: (1) preparing protein peptide enzymatic hydrolysate: carrying out blood dissolving, enzymolysis, decoloration and ultrafiltration on fresh pig blood as a raw material; (2) mixing chitosan, selenium compounds and citric acid in a certain proportion, and preparing a nano-selenium solution in a water phase; (3) adding the nano-selenium solution into the protein peptide enzymatic hydrolysate with the temperature of 55-65 DEG C so as to obtain mixed liquid, heating the mixed liquid to 45-60 DEG C, and reacting for 1-2 hours, so as to obtain finished liquid; and (4) concentrating and drying the finished liquid, so as to obtain the finished product. The selenium-rich protein peptide leaf fertilizer is rich in high-activity oligopeptides and free amino acids as well as selenium and chitosan, can be rapidly and thoroughly dissolved, can be easily absorbed by crops and can be rapidly conveyed in the crops, so that the problem that the leaf fertilizer is difficult to absorb is solved, the growth speed and the disease resistance of the crops are greatly increased, and the crop quality is improved.

Description

technical field [0001] The invention relates to the field of dietary nutrition, in particular to a preparation method of selenoprotein peptide foliar fertilizer. Background technique [0002] Foliar fertilizers are often used in agriculture to quickly supplement the nutrients needed by plants. Because of their convenience, quickness, better absorption effect and less waste than root fertilization, various nutritional or functional foliar fertilizers have gradually entered the market in recent years. However, in recent years, most of the foliar fertilizers produced in China have low effective content and single function, and most of them are to supplement trace elements for crops, and have single nutritional components. The Chinese patent with the patent number CN101891520 disclosed an amino acid foliar fertilizer on August 29, 2012, which is prepared from the following materials in terms of mass percentage: 30%-50% plant amino acids, 1%-5% compound nitrophenol Sodium, 15%-2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 陈林其他发明人请求不公开姓名
Owner CHONGQING SANLINGSAN TECH CO LTD
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