Preparation method of selenium-rich protein peptide leaf fertilizer
A technology of selenium-enriched protein and foliar fertilizer, applied in fertilization device, fertilizer mixture, application and other directions, can solve the problems of low element utilization rate, unstable effect, single nutrient composition, etc., to improve the quality and added value of crops. The effect of high, high protein content
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Embodiment 1
[0024] A method for preparing selenoprotein peptide foliar fertilizer, the steps are as follows: (1) Preparation of protein peptide enzymatic hydrolysis solution: a) collect fresh pig blood, anticoagulate with 5g / L sodium citrate, and heat at 4°C, 8000r Centrifuge for 10 minutes per minute, collect the lower layer of sediment to obtain red blood cells; add the same volume of normal saline to the red blood cells to wash and centrifuge twice, then add 2 times the volume of distilled water, ultrasonic cell disruption assists hemolysis for 20 minutes, and then centrifuge at 4°C and 4000r / min After 10 minutes, the hemoglobin solution was obtained, and stored at 4°C for later use; b) Take the hemoglobin solution, add an appropriate amount of trypsin to enzymolyze it, stir, and maintain the pH of the system with 2mol / L NaoH solution at 45-50°C for 6-10 hours; The reaction product is rapidly heated to 85°C and maintained at this temperature for 10-15 minutes, then rapidly cooled to bel...
Embodiment 2
[0027] A preparation method of selenoprotein peptide foliar fertilizer, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as in Example 1; (2) Carboxymethyl chitosan, sodium selenite and citric acid are prepared according to the The mass ratio is 100:1:15, and the nano-selenium solution is prepared in the water phase, the temperature is controlled at 60 °C, and the mass fraction of chitosan in the solution is 5×10 -4 , the concentration of selenium compound is 3mmol / L, and the concentration of citric acid is 7g / L; (3) Add the nano-selenium solution to the enzymatic hydrolysis solution at a temperature of 55°C for 2 hours to obtain a liquid; (4) Add the The finished product is obtained by concentrating the liquid and spray drying, wherein the air inlet temperature is 220°C and the air outlet temperature is 90°C during spray drying.
Embodiment 3
[0029] A preparation method of selenoprotein peptide foliar fertilizer, the steps are as follows: (1) The preparation of protein peptide enzymatic hydrolysis solution is the same as in Example 1; (2) Carboxymethyl chitosan, sodium selenite and vitamin C are prepared according to the The mass ratio is 90:1:12, and the nano-selenium solution is prepared in the water phase, and the temperature is controlled at 65°C for 2 hours, and then the ultrasonic wave is 100W, 15kHz for 30 minutes, and the treatment is 6 minutes and 7 minutes. The quality of chitosan in the solution The fraction is 5×10-4, the concentration of selenium compound is 3mmol / L, and the concentration of citric acid is 6g / L; (3) Add the nano-selenium solution into the enzymatic hydrolysis solution at a temperature of 60°C, and chelate for 1.5h to obtain into a liquid; (4) concentrating the liquid and spray drying to obtain a finished product, wherein the temperature of the air inlet during spray drying is 230°C and ...
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