Self-repairing injectable bone cement and preparation method

Abstract

The invention discloses self-repairing injectable bone cement and a preparation method thereof. Solid powder of the self-repairing injectable bone cement contains inorganic calcium salt and is composited with formylated alginic acid or formylated alginic acid components, cured liquid contains water-soluble lysine-based polymers, and the self-repairing injectable bone cement is obtained by blending the cured liquid and the solid powder according to the liquid-solid ratio of 0.1 mL to 10 g. According to the self-repairing injectable bone cement material, amino groups and formyl groups are introduced to react to form imine bonds, a cross-linked network with the self-repairing property can be established, the mechanical strength and the collapse resistance property are improved, and meanwhile the good injection property can be reserved, so that the product has the good comprehensive property. By means of the good biocompatibility of lysine primitives and the degradability of the water-soluble lysine-based polymers and sodium alginate, adhesion, growth and proliferation of cells are facilitated, bone repair is facilitated, and ingrowth of the cells and blood vessels and bone tissue reconstruction are achieved.

Description

technical field

[0001] The invention relates to the field of bone cement, in particular to a self-repairing injectable bone cement and a preparation method thereof. Background technique

[0002] Bone cement can be molded arbitrarily at the natural temperature and internal environment of the human body, solidifies by itself, and has no obvious exothermic effect, and can eventually be converted into a substance close to the inorganic components of human bone tissue. It has good biocompatibility and high safety, and can be used It is degraded in the liquid phase of the body and absorbed by bone tissue.

[0003] The plasticity of the bone cement material makes it popular in the field of injection materials. The material can be directly injected into the bone defect and solidified quickly. It does not need to be plasticized in advance and can be implanted minimally. The trauma surface is small and many other advantages.

[0004] In view of the fact that most inorganic salt bone...

Examples

preparation example Construction

[0080] 2. A preparation method of the self-repairing injectable bone cement, comprising the following steps

[0081] Step a: Preparation of component (I) bone cement powder: uniformly mix inorganic calcium salt powder with alginic acid or alginate;

[0082] Step b: Preparation of component (II) solidified liquid: dissolving the water-soluble lysine-based polymer in water, an aqueous solution, or a mixed solvent compatible with water.

[0083] Step c: mixing and curing: mixing component (I) and component (II) at a liquid-solid ratio of 0.1-10 mL:g to obtain the self-repairing injectable bone cement.

[0084] 2.1 Preparation of component (I)

[0085] Conventional preparation methods of inorganic calcium salts are well known to those skilled in the art and will not be repeated here. Taking calcium phosphate as an example, amorphous raw materials are usually prepared, and then sintered to obtain the desired crystal phase structure. The preparation of amorphous calcium phosphate i...

Embodiment 1

[0103] 1. Preparation of Bone Cement Powder

[0104] Calcium phosphate bone cement powder: Phosphorus-based calcium salt bone cement powder composed of β-tricalcium phosphate / tetracalcium phosphate in a molar ratio of 2:1.

[0105] Preparation of alginylated sodium alginate

[0106] (a) Add sodium alginate with a viscosity of 400mpa·s into the flask, add deionized water to dissolve it completely, and mix sodium periodate and potassium periodate (molar ratio 1:1) in another beaker Dissolve completely in water, then slowly add the obtained periodate solution into the sodium alginate solution according to the molar ratio of sodium alginate and periodate of 1:0.5, and stir magnetically at 25°C in the dark, and react for 8 hours;

[0107] (b) After the reaction is completed, add excess ethylene glycol to the resulting solution, stir magnetically for 1.5 hours to remove unreacted periodate, then put it into a dialysis bag with a molecular weight cut-off of 3000, and carry out the r...

Embodiment 2

[0117] 1. Preparation of bone cement powder: Prepare calcium sulfate hemihydrate, tetracalcium phosphate, and strontium hydrogen phosphate composite bone cement powder by ball milling, wherein the mass percentage of calcium sulfate hemihydrate is 80%, and the moles of tetracalcium phosphate and strontium hydrogen phosphate The ratio is 1:1. Formylated alginic acid with a mass fraction of 6% and an oxidation degree of about 70% was added by blending.

[0118] 2. Preparation of solidification solution: α-polylysine with a molecular weight of about 2000 Da was dissolved in a glycine-sodium hydroxide buffer solution with a pH of 9.0, with a mass fraction of 8%.

[0119] 3. Use a liquid-solid ratio of 0.5mL / g (the molar ratio of aldehyde group: amino group is about 1:1) for reconciliation, put it into a 1mL disposable syringe, and manually push the injection. The injection time is about 6 minutes, and the curing time is about 30 minutes.

[0120] Cured at room temperature for 24 h...

technical field

[0001] The invention relates to the field of bone cement, in particular to a self-repairing injectable bone cement and a preparation method thereof. Background technique

[0002] Bone cement can be molded arbitrarily at the natural temperature and internal environment of the human body, solidifies by itself, and has no obvious exothermic effect, and can eventually be converted into a substance close to the inorganic components of human bone tissue. It has good biocompatibility and high safety, and can be used It is degraded in the liquid phase of the body and absorbed by bone tissue.

[0003] The plasticity of the bone cement material makes it popular in the field of injection materials. The material can be directly injected into the bone defect and solidified quickly. It does not need to be plasticized in advance and can be implanted minimally. The trauma surface is small and many other advantages.

[0004] In view of the fact that most inorganic salt bone...

Examples

preparation example Construction

[0080] 2. A preparation method of the self-repairing injectable bone cement, comprising the following steps

[0081] Step a: Preparation of component (I) bone cement powder: uniformly mix inorganic calcium salt powder with alginic acid or alginate;

[0082] Step b: Preparation of component (II) solidified liquid: dissolving the water-soluble lysine-based polymer in water, an aqueous solution, or a mixed solvent compatible with water.

[0083] Step c: mixing and curing: mixing component (I) and component (II) at a liquid-solid ratio of 0.1-10 mL:g to obtain the self-repairing injectable bone cement.

[0084] 2.1 Preparation of component (I)

[0085] Conventional preparation methods of inorganic calcium salts are well known to those skilled in the art and will not be repeated here. Taking calcium phosphate as an example, amorphous raw materials are usually prepared, and then sintered to obtain the desired crystal phase structure. The preparation of amorphous calcium phosphate i...

Embodiment 1

[0103] 1. Preparation of Bone Cement Powder

[0104] Calcium phosphate bone cement powder: Phosphorus-based calcium salt bone cement powder composed of β-tricalcium phosphate / tetracalcium phosphate in a molar ratio of 2:1.

[0105] Preparation of alginylated sodium alginate

[0106] (a) Add sodium alginate with a viscosity of 400mpa·s into the flask, add deionized water to dissolve it completely, and mix sodium periodate and potassium periodate (molar ratio 1:1) in another beaker Dissolve completely in water, then slowly add the obtained periodate solution into the sodium alginate solution according to the molar ratio of sodium alginate and periodate of 1:0.5, and stir magnetically at 25°C in the dark, and react for 8 hours;

[0107] (b) After the reaction is completed, add excess ethylene glycol to the resulting solution, stir magnetically for 1.5 hours to remove unreacted periodate, then put it into a dialysis bag with a molecular weight cut-off of 3000, and carry out the r...

Embodiment 2

[0117] 1. Preparation of bone cement powder: Prepare calcium sulfate hemihydrate, tetracalcium phosphate, and strontium hydrogen phosphate composite bone cement powder by ball milling, wherein the mass percentage of calcium sulfate hemihydrate is 80%, and the moles of tetracalcium phosphate and strontium hydrogen phosphate The ratio is 1:1. Formylated alginic acid with a mass fraction of 6% and an oxidation degree of about 70% was added by blending.

[0118] 2. Preparation of solidification solution: α-polylysine with a molecular weight of about 2000 Da was dissolved in a glycine-sodium hydroxide buffer solution with a pH of 9.0, with a mass fraction of 8%.

[0119] 3. Use a liquid-solid ratio of 0.5mL / g (the molar ratio of aldehyde group: amino group is about 1:1) for reconciliation, put it into a 1mL disposable syringe, and manually push the injection. The injection time is about 6 minutes, and the curing time is about 30 minutes.

[0120] Cured at room temperature for 24 h...