A kind of Aspergillus niger strain and application thereof

A strain of Aspergillus niger, the technology of Aspergillus niger, applied in the field of microorganisms, can solve the problems of increasing the purification and blending process, increasing labor and cost, etc., and achieves the effects of good enzyme production effect, convenient operation and reduced difficulty

Inactive Publication Date: 2019-08-16
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the production of compound enzyme preparations is mainly obtained through the blending of multiple single enzyme preparations or the fermentation of multiple enzyme-producing strains, but there are few reports on the production of compound enzyme preparations by fermentation of a single multi-enzyme-producing strain.
Wherein the method for the blending of a single enzyme preparation increases the purification and blending process of the enzyme in the production process of the compound enzyme preparation, which increases the amount of labor and cost; the mixed fermentation method of multiple enzyme-producing strains mainly relies on the synergy between different bacterial strains to There are higher requirements for raw materials and fermentation conditions; multi-enzyme single strain fermentation to produce complex enzymes is the simplest and easy-to-achieve method, but it is necessary to vigorously develop excellent strains that produce complex enzymes

Method used

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  • A kind of Aspergillus niger strain and application thereof
  • A kind of Aspergillus niger strain and application thereof
  • A kind of Aspergillus niger strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] (1) Acquisition of strains

[0033] Separation and screening are from the soil humus layer of Taibai Mountain. The medium used for separation and screening is sodium carboxymethyl cellulose screening medium. The specific components are as follows: sodium carboxymethyl cellulose 10.0g, KH 2 PO 4 1.0g, NaCl 0.1g, MgSO 4 7H 2 O 0.3g, NaNO 3 2.5g, FeCl 3 0.001g, CaCl 2 0.1g, Congo red 0.2g, agar 18g, H 2 O 1000mL, pH natural.

[0034] (2) Culture characteristics of the strain

[0035]The Aspergillus niger bacterial strain of the present invention is cultured on PDA agar medium, under 25 ℃ of dark conditions for 7 days, colony diameter 70mm, initial texture is nearly fluffy, and colony is circular, flat, dense, stout, orderly, after producing spores with powder granular, such as figure 1 shown. Wherein, the composition of PDA agar medium is: potato 200g, glucose 20g, agar 20g, distilled water 1000ml, pH is natural.

[0036] (3) Morphological characteristics o...

Embodiment 2

[0042] The Aspergillus niger (Aspergillus niger) Z-9 bacterial strain of the present invention can use crop stalks as a carbon source to produce cellulase through liquid fermentation. Put it into sterilized 80mL liquid fermentation medium, cultivate it on a shaker at 200r / min at 30°C for 5 days, and obtain a cellulase-containing fermentation broth, which is centrifuged at 8000r / min and suction-filtered to obtain crude Enzyme solution; wherein, the composition of the liquid fermentation medium is: wheat straw powder 2g, bran 20g, microcrystalline cellulose 6g, bean cake powder 5g, ammonium sulfate 2g, urea 1g, sodium nitrate 2g, potassium dihydrogen sulfate 3g, magnesium sulfate 0.5g, Tween 803mL, tap water 1000mL, pH natural.

[0043] The crude enzyme solution was determined, in which the cellulase filter paper enzyme activity (FPA) was 64.67U / mL, and it also contained xylanase and pectinase, the enzyme activities were 216.70U / mL and 66.91U / mL respectively, and the FPA enzyme ...

Embodiment 3

[0046] Preparation of Aspergillus niger (Aspergillus niger) Z-9 spore preparation: Weigh 50g of bran in a 650ml tissue culture bottle, add 60ml of tap water, sterilize at 121°C for 30min, inoculate 5ml of Aspergillus niger spore suspension after cooling, stir well, and use Four layers of sterile gauze were sealed, and cultured at a constant temperature of 28°C. After 24 hours of culture, mycelia began to grow in large quantities. After 36 hours of culture, the mycelia were covered with fermentation substrates. The results of sampling test showed that the germination rate of Aspergillus niger Z-9 spores reached 99.5%, and the Aspergillus niger spores had the characteristics of easy preparation, survival and cultivation.

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Abstract

The invention belongs to the technical field of microorganisms, and discloses an Aspergillus niger strain, which is identified as Aspergillus niger (Aspergillus niger) Z-9, and whose preservation number is CGMCC No.11130 in the General Microorganism Center of China Microbiological Culture Collection Management Committee. Also disclosed is the application of the Aspergillus niger, which can produce cellulase, pectinase and xylanase by fermentation, can be used to prepare cellulase, xylanase or pectinase preparations, and can also be used to prepare at least The compound enzyme preparation of cellulase, xylanase or / and pectinase expands its application range in feed enzyme preparation; it can be used in biological fermentation feed, and can effectively modify pomace substances into biological protein feed , enhance the added value of pomace, provide excellent materials for the feed application of pomace and other dregs, and the prepared biological protein feed has a good application effect on breeding animals such as dairy cows, sheep and piglets.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an Aspergillus niger strain and an application thereof. Background technique [0002] Feed enzyme preparation is a kind of feed additive with enzyme as the main functional factor and processed through a specific production process. In the middle of the last century, with the deepening of research on the structure of enzyme protein, enzyme kinetics, enzyme mode of action, and enzyme synthesis and regulation mechanism, the research and production of enzyme preparations also developed rapidly. Jesen used crude enzyme preparations in poultry barley rations, which significantly improved the feeding effect of rations and improved the growth performance of poultry. So far, various countries have opened the prelude to the research on feed enzymes. In 1975, the American Kemin Company launched commercial feed enzyme preparations, marking the beginning of exogenous enzymes as feed a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/42C12N9/24C12N9/88A23K10/37A23K10/12C12R1/685
CPCC12N9/2402C12N9/2437C12N9/248C12N9/88C12Y402/0201C12N1/145C12R2001/685Y02P60/87
Inventor 李海洋来航线韦小敏李肖薛泉宏
Owner NORTHWEST A & F UNIV
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