Melanin precursor substance hair dye and using method thereof

A technology of melanin precursor and hair dye, which is applied in fermentation, hair care, pharmaceutical formulation and other directions, can solve the problems of unstable performance, high cost, long time consumption, etc., and achieves safe and non-toxic use effect, reliable use effect and safety. high effect

Active Publication Date: 2016-11-16
珠海丝彩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide to hair dyes, and lack mature pigment extraction technology.
Known to have the method (patent document CN101164528A) of utilizing plant pigment catechol to prepare natural hair dye at present, but the catechol used wherein is to be extracted from catechin of Chinese medicinal material, and its shortcoming is that the planting scale of Chinese medicinal material is limited, and the pigment extraction cost Relatively high; Also have the report to utilize the catechol oxidase in the filamentous fungus cell of expressing mold to oxidize dopa and

Method used

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  • Melanin precursor substance hair dye and using method thereof
  • Melanin precursor substance hair dye and using method thereof
  • Melanin precursor substance hair dye and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0078] 1. Extraction and determination of tyrosinase

[0079] Take 500 grams of crushed Pleurotus eryngii and add 1000 ml of distilled water to extract the tyrosinase solution or use 1000 ml of neutral phosphate buffer to extract the tyrosinase solution.

[0080] The measurement method of tyrosinase activity is as follows: Take 0.1ml of mushroom compound enzyme solution and 3.9ml of substrate solution to react at 317nm for 10min, and the enzyme activity unit U is defined as 0.01 units of absorbance change per 1min is 1 enzyme activity unit. When the measured result is greater than 1000U, the enzyme solution can be used for the following melanin precursor accumulation reaction.

[0081] 2. Accumulation reaction of melanin precursor

[0082] Prepare 2L of tyrosine solution with a concentration of 1mM-10mM, add the tyrosine substrate solution into a bior...

Embodiment 2

[0089] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0090] 1. Extraction of Tyrosinase

[0091] Use a juice extractor to crush the mushrooms, add 500g fruiting body residue to 1000ml, extract the enzyme solution with a citric acid buffer solution with a pH value of 6.8, use 5% activated carbon to absorb and treat, and then centrifuge for later use.

[0092] 2. Immobilization of Tyrosinase

[0093] 200-mesh diatomite was heat-treated at 60°C for 30 minutes, soaked in phosphate buffer for 20 minutes, centrifuged to remove the supernatant, and the treated diatomite was added to the above enzyme solution for 5 hours to obtain immobilized enzyme for use .

[0094] 3. Accumulation reaction of melanin precursors

[0095] Prepare 2L of tyrosine solution with a concentration of 5mM, which can be completely dissolved by heating and dissolving.

[0096] Add the tyrosine substrate solution into a bioreactor with ...

Embodiment 3

[0103] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0104] 1. Extraction of Tyrosinase

[0105] Use a juice extractor to crush Agaricus bisporus, add 500g of crushed residue to 1000ml of Tris-hydrochloric acid buffer solution with a pH value of 7.0 to extract the enzyme solution, centrifuge to extract the supernatant, and use 10% macroporous adsorption resin to adsorb polysaccharides and miscellaneous proteins in the enzyme solution. The treated enzyme solution was stored at 4°C for later use.

[0106] 2. Accumulation reaction of melanin precursor

[0107] Prepare 2L of tyrosine solution with a concentration of 10mM, which can be completely dissolved by heating and dissolving.

[0108] Reaction process is with embodiment 1.

[0109] The liquid sample taken out was filtered through filter paper, and after the filtrate was diluted 10 times, the absorbance at 300 nm and 475 nm was measured in an ultraviol...

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Abstract

The invention discloses a melanin precursor substance hair dye. The melanin precursor substance hair dye consists of a melanin precursor, a common hair dye adjuvant and water, wherein the melanin precursor includes dopachrome, 5,6-dihydroxyindole and 5,6-dihydroxyindole-2-carboxylic acid; the melanin precursor accounts for 0.01-5% of the hair dye in percentage by weight; and the common hair dye adjuvant includes one or more of a wetting penetrating agent, a thickening agent, a surfactant, a moisturizer and a pH value regulating agent. The hair dye disclosed by the invention combines the advantages of a chemically synthetic hair dye and a natural hair dye; by extracting complex enzyme liquid in natural edible fungi, such melanin precursor substances as the dopachrome, the 5,6-dihydroxyindole, the 5,6-dihydroxyindole-2-carboxylic acid and the like are synthesized through bio-conversion by virtue of amino acid, so that the hair dye is free from irritation and allergy to skin, high in safety and capable of keeping a natural black hair dyeing effect; and the hair dye has huge popularization and application values in the field of the preparation of hair dyes.

Description

technical field [0001] The invention relates to a hair dye prepared by enzyme treatment, in particular to a method for extracting edible fungus enzyme liquid, converting an amino acid substrate into a natural melanin precursor, and preparing it into a non-oxidative biological hair dye. Background technique [0002] At present, with the continuous improvement of people's attention to health, chemically synthesized hair dyes contain carcinogenic and strong allergenic chemical dyeing ingredients (such as phenylenediamines, aminophenols, etc.) and some harmful ingredients (such as hydrogen peroxide) , ammonia, etc.), has been more and more people's attention. For this reason, people try to seek natural harmless, safer hair dyeing materials. For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide in hair dyes, and lack mature pigment ext...

Claims

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Application Information

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IPC IPC(8): A61K8/49A61K8/44A61K8/41C12P17/10A61Q5/10
CPCA61K8/41A61K8/44A61K8/492A61K2800/4324A61Q5/10C12P17/10
Inventor 张然薛俊厉秀茹
Owner 珠海丝彩生物科技有限公司
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