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Method for preparing yeast autolysate from single-cell protein complex enzyme preparation cultured by food and beverage production wastewater and produced yeast product

A technology for compound enzyme preparation and waste water production, which is applied in the production and application of single-cell protein, can solve the problems of low yeast autolysis efficiency, etc., and achieve the effects of reducing the operation of the insulation system, high biological safety, and improving the purity of the enzyme.

Inactive Publication Date: 2016-11-16
ICELL SUSTAINABLE SHANGHAI NUTRITION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0028] The purpose of the present invention is to solve the defect of low efficiency of existing yeast autolysis, and provide a high-efficiency, active ingredient-rich production method using endogenous enzyme-rich single-cell protein and yeast mixed autolysis

Method used

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  • Method for preparing yeast autolysate from single-cell protein complex enzyme preparation cultured by food and beverage production wastewater and produced yeast product
  • Method for preparing yeast autolysate from single-cell protein complex enzyme preparation cultured by food and beverage production wastewater and produced yeast product
  • Method for preparing yeast autolysate from single-cell protein complex enzyme preparation cultured by food and beverage production wastewater and produced yeast product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Embodiment 1: Utilize beer wastewater to produce single-cell compound enzyme preparation for enzymatic hydrolysis of protein feed

[0129] Raw materials: The main components of beer wastewater are: sugars, alcohols, amino acids, pectin, hops, vitamins, protein compounds, organic matter and a small amount of inorganic salts in the packaging workshop, etc. BOD / COD is higher, with an average of 0.55. And there are a lot of suspended matter, such as wheat dregs, etc., and there are often alkaline cleaning agents and fungicides that are put into the disinfection and cleaning process. The water quantity and quality of beer wastewater depends on the production cycle, and the water quantity and quality fluctuate greatly. The amount of wastewater in the production period is huge, the COD is 2000-4000mg / L, and the pH value is mainly slightly alkaline to medium alkaline.

[0130] Production Process:

[0131] The production process uses the existing wastewater treatment system o...

Embodiment 2

[0154] Embodiment 2. The production of molasses glutamic acid production waste water is used for the single-cell composite enzyme preparation of protein feed enzymolysis

[0155] Raw materials: The specific characteristics of the wastewater produced by molasses glutamic acid are as follows: the waste liquid is brown in appearance, with a small amount of bubbles on the surface, strong acidity, high concentration of organic matter, COD load as high as 30000-70000mg / L, BOD load as high as 20000-42000mg / L , high content of suspended solids, 12000-20000mg / L. Wastewater contains a large amount of organic acids by-products of microbial metabolism, mainly lactic acid, succinic acid, etc. The total amount of amino acids in molasses glutamic acid wastewater is as high as 7%. Except for residual glutamic acid, other residual amino acids mainly include aspartic acid, Alanine, glycine, valine, leucine, isoleucine, phenylpropoxyacid, lysine, arginine, etc., and residual sugar, urea, etc. in...

Embodiment 3

[0192] Embodiment 3: This example is the manufacturing example of raw material selection, autolysis production

[0193] In this example, the compound flora rich in endogenous enzymes cultivated in real time from the organic wastewater of the beer factory is used as the raw material of the single-cell compound enzyme preparation. The water content is 90% to 98%, and the water content is reduced to 80% to 85% after centrifugal dehydration. The protein content (dry basis) of the prepared single-cell composite enzyme preparation is higher than 50%, and it is transported to Autodissolving equipment.

[0194] The yeast is commercially available common dried non-autolyzed beer yeast with a protein content of 50%.

[0195] Add the prepared single-cell compound enzyme preparation and yeast at a ratio of 50% / 50% of dry matter, and the addition method of the ratio is realized by the frequency of the screw conveying pump and the motor of conveying Jiaolong.

[0196] In addition to the c...

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Abstract

The invention provides a method for preparing yeast autolysate from a single-cell protein complex enzyme preparation cultured by food and beverage production wastewater. The method comprises the following steps that the single-cell protein complex enzyme preparation and yeast are mixed on the basis of the dry biomass being (2:8)-(8:2), autolyzed and then dried and sterilized, autolysis is divided into the low temperature section and the high temperature section, the high temperature section is kept for 30-120 min under the temperature of 75-95 DEG C, and the method for preparing yeast autolysate from the single-cell protein complex enzyme preparation cultured by food and beverage production wastewater comprises the following steps that 1, the food and beverage production wastewater is pretreated; 2, nutrition adjustment is performed on the pretreated food beverage production wastewater; 3, microorganism culture is performed; 4, bacterial sludge is collected to obtain the single-cell protein complex enzyme preparation. The method has the important environmental protection significance, and has the great economic value.

Description

technical field [0001] The invention relates to the technical field of single-cell protein production and application. Background technique [0002] Single cell protein (SCP for short), also known as microbial protein or bacterial protein, uses industrial wastewater, waste gas, natural gas, petroleum alkanes, agricultural and sideline processing products, and organic waste as culture media to cultivate yeast, non-pathogenic Single-celled organisms such as bacteria, microbes, and fungi. Single-cell protein is not a pure protein, but a cytoplasmic mass composed of a mixture of proteins, fats, carbohydrates, nucleic acids, and non-protein nitrogenous compounds, vitamins, and inorganic compounds. The more important single-cell proteins are yeast protein, bacterial protein and algae protein, and their chemical composition is generally dominated by protein and fat. [0003] Nutrients such as proteins and amino acids contained in single-cell proteins can be directly applied to pr...

Claims

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Application Information

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IPC IPC(8): C12N1/06
CPCC12N1/06
Inventor 曾靖婷
Owner ICELL SUSTAINABLE SHANGHAI NUTRITION CO LTD
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