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Diglyceride oil composition rich in catalpic acid and preparation method and application thereof

A technology of diglyceride oil and diglyceride, applied in the field of applied chemistry, can solve the problems of low utilization rate of raw materials, high price of lipase, increased oxidation, etc., achieve high food safety, simple preparation process, and improve blood lipids Effect

Inactive Publication Date: 2016-11-23
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the preparation method of diglyceride mainly contains following several kinds: (1) triglyceride alcoholysis method: promptly under the catalysis of lipase, triglyceride and lower alcohol react, generate diglyceride and lower alcohol ester, but this The lipases Novozyme 435 and Lipozyme IM used in the method can only specifically decompose or synthesize the ester bond at the sn-1 or sn-3 position, so only 1,2-diglyceride can be obtained
(2) Partial hydrolysis and esterification method: that is, first partially hydrolyze triglyceride to obtain fatty acid, then add glycerol, under the catalysis of sn-1 and sn-3 position selective lipase, glycerol and the fatty acid obtained by partial hydrolysis generate ester Chemical reaction to generate 1,3-diglyceride. Most of the current diglycerides, including EnovaTM Oil produced by Kao Company, are developed based on this principle. The biggest disadvantage of this method is that the process is complicated and requires multi-step separation. In addition The utilization rate of raw materials is low, and lipase needs to be used in addition, but the price of lipase is relatively high, and there are problems such as high cost for industrial scale production of diglycerides
(3) Glycerol hydrolysis method: mixing triglyceride oil with glycerin and an alkali metal salt or alkaline earth metal salt containing a monocarboxylic acid or a dicarboxylic acid or a mixture thereof (as a catalyst), through alcoholysis of triglyceride , to produce commercial, food-grade 1,3-diglyceride oil, but the conventional glycerolysis needs to be reacted at a high temperature of 220-260 ° C, so that the oxidation of triglycerides, diglycerides and fatty acids, especially unsaturated fatty acids increase
Not only do consumers have concerns about the safety of industrially prepared CLA, but there are also many problems in the industrialization of CLA itself, such as: the cost of industrialized production of CLA is relatively high; industrially prepared CLA has multiple isomer forms, not Each isomer has beneficial functions to the human body, among which cis9, trans11-CLA is the active isomer with higher safety

Method used

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  • Diglyceride oil composition rich in catalpic acid and preparation method and application thereof
  • Diglyceride oil composition rich in catalpic acid and preparation method and application thereof
  • Diglyceride oil composition rich in catalpic acid and preparation method and application thereof

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Effect test

Embodiment 1

[0029] Collect catalpa seeds, dry them in an oven at 40°C, and crush them to obtain 1,000 g of dry powder. Reflux extraction with 5 times the volume of petroleum ether at a temperature lower than 60°C for 6 hours to obtain a green crude triglyceride extract with an oil yield of 28.2%. . Take 50 mL of crude triglyceride extract, add 200 mL of 2M 90% potassium hydroxide ethanol solution, saponify at 60°C under nitrogen protection for 2 hours, add 50 mL of water, then add 50 mL of petroleum ether for three extractions to remove non-soap compounds, and then add 6M hydrochloric acid After 100mL neutralization and acidification, the upper layer was extracted with petroleum ether to obtain a petroleum ether solution, and then the crude extract of catallic acid was obtained after vacuum rotary evaporation. The crude extract of catalpalic acid was dissolved in ethanol, crystallized at 4°C under normal pressure for 8 hours to remove saturated fatty acids, and the obtained ethanol soluti...

Embodiment 2

[0033] Collect catalpa seeds, dry them naturally in the sun, and crush them to obtain 1000 g of dry powder with a particle size of 0.4-0.5 mm. Extract with supercritical CO2 (HA221-50- produced by Huaan Supercritical Extraction Unit, Nantong City, Jiangsu Province) at 30 MPa and 40 ° C. 06 type supercritical extraction device) for 2 hours, the green triglyceride crude extract (crude oil) was obtained at a separation pressure of 15MPa and a separation temperature of 50°C, with an oil yield of 30.2%; the crude oil was degummed, deacidified, decolorized, Triglycerides are obtained by refining after deodorization and dewaxing. Take 50 mL of crude triglyceride extract, add 200 mL of 2M 90% potassium hydroxide ethanol solution, saponify at 60°C under nitrogen protection for 2 hours, add 50 mL of water, then add 50 mL of petroleum ether for three extractions to remove non-soap compounds, and then add 6M hydrochloric acid After 100mL neutralization and acidification, the upper layer w...

Embodiment 3

[0037] Collect catalpa seeds, dry them in an oven at 40°C, and crush them to obtain 1000 g of dry powder. Extract them with 5 times the volume of a mixed solution of chloroform and methanol (volume ratio: 1:1) at room temperature for 24 hours to obtain green catalpalic acid triglycerides. The crude ester extract has an oil yield of 29.7%. Take 100mL of crude triglyceride extract, add 400mL of 2M 90% sodium hydroxide ethanol solution, saponify at 60°C under nitrogen protection for 2h, add 1000mL of water, then add 100mL of petroleum ether for three extractions to remove non-soap compounds, and then add 6M hydrochloric acid After neutralizing and acidifying 200mL, extract the upper layer with petroleum ether to obtain a petroleum ether solution. The crude extract of catalpalic acid was crystallized at 4°C under normal pressure for 10 hours to remove saturated fatty acids, and then crystallized at minus 40°C under normal pressure for 12 hours. Repeat 4 High-purity catallic acid w...

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Abstract

The invention relates to a diglyceride oil composition rich in catalpic acid; the diglyceride oil composition is composed of the following components according to the mass percentage: 50-90% of diglyceride, 9-45% of triglyceride, 0.1-10% of monoglyceride and 0.02-10% of residues; in the total glycerides composed of monoglyceride, diglyceride and triglyceride, the mass ratio of catalpic acid to fatty acid is (0.2-0.8):1, and the residues comprise free fatty acid and glycerol. The invention also relates to a preparation method of the composition and an application of the composition in functional food and drugs for controlling weight, improving blood lipid and increasing the immunity. The diglyceride oil composition rich in catalpic acid not only has an anti-obesity efficacy of diglyceride but also has anti-inflammatory and anti-obesity efficacies of catalpic acid, so a large number of the composition can be applied in the functional food and drugs for controlling weight, improving blood lipid and increasing the immunity; and in short, the preparation process of the composition is simple, is low in cost, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of applied chemistry, in particular to a diglyceride oil composition rich in catallic acid, a preparation method and application thereof. Background technique [0002] Diacylglycerol is a structural lipid in which a fatty acid in a triacylglycerol is substituted by a hydroxyl group. It is a trace component of natural vegetable oils and the endogenous middle of fat metabolism in the body. It has two natural isomerisms Constructs, namely 1,2-diglyceride and 1,3-diglyceride, as shown in Formula 1. [0003] [0004] Schematic structure of diglyceride in formula 1 (wherein COR and COR' are saturated or unsaturated fatty acyl) [0005] At present, after a number of safety assessments, it is found that diglyceride is non-toxic and harmless to the human body, and it has been recognized as a "generally recognized safe" substance by the US FDA. In 2009, the Ministry of Health of the People's Republic of China appr...

Claims

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Application Information

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IPC IPC(8): A23L1/30C11B1/04C11B1/06C11B1/10C11B3/00A61K31/202A61P3/04A61P3/06A61P37/04C07C57/12C07C27/02
Inventor 袁高峰孙海燕陈小娥
Owner ZHEJIANG OCEAN UNIV
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