31 results about "Catalpic acid" patented technology

The invention provides a biocatalysis method for preparing pyruvic acid from L-alanine. The method comprises the steps of: carrying out permeability treatment on Alcaligens faecalisl.1799 cells; catalyzing oxidative deamination of L-alanine by utilizing L-amino acid oxidase in the cells in the presence of air to form pyruvic acid, ammonia and hydrogen peroxide; catalyzing the hydrogen peroxide formed in the reaction by utilizing catalase in the cells to realize pyruvic acid accumulation without producing by-products. The method provided by the invention has the advantages of low cost, high product yield and purity and environment friendliness, and is suitable for industrial production of pyruvic acid.

The invention relates to a mixture with aliphatic acid carbethoxy sulfonate salt, and relative production, wherein said mixture comprises 90-98 deals of aliphatic acid sulfonate salt, 1-4 deals of aliphatic acid sulfonate salt, 0.5-4 deals of sulfate, 0.1-2 deals of fatty acid ester, 0.3-3 deals of ethylsulfuric acid salt, and 1-50 deals of water. The production comprises that: 1, sulfonating the fatty acid ester and sulfur trioxide at 1:1-1:1. 1 mol ratios; 2, adding alcohol into mixture to be aged; 3, adding auricome into mixture to poach; 4, adding extractive into the mixture to extract; 5, adding alkali metal mixture into mixture to be neutralized to obtain the mixture. The inventive production is safe while the product purity is higher than 90%.

The invention relates to a harmless extraction process for preparing agricultural biochemical fulvic acid from livestock and poultry manure. The process includes an acid-soluble extraction process of fulvic acid and a Fenton oxidation degradation process. The process of acid-soluble extraction of fulvic acid is to combine the acid-soluble extractant of group A containing 0.1%~2.5% ferrous sulfate, pH value ≤ 2, and 1%~2.5% sulfuric acid with fermented livestock and poultry manure at a ratio of 1:5~15 The proportion of the solution is mixed into a suspension solution, stirred at room temperature for 30min~60min, the dissolved fulvic acid contains a chelate of organic acid and ferrous ion; the Fenton oxidation degradation process is to extract the material through acid dissolution, and Value ≤ 2, containing 15% hydrogen peroxide and B group acid soluble extractant containing 1% ~ 2.5% sulfuric acid mixed, the control material contains 0.5% ~ 3.0% hydrogen peroxide, after the two groups of materials are mixed, chlorine peroxide is Under the catalysis of ferrous ions, the Fenton oxidation degradation reaction is generated, and the residual antibiotics, hormones, pesticides, and pathogenic bacteria, decay-causing bacteria, and parasite eggs are rapidly degraded to achieve the effect of harmless extraction.

The invention discloses a treatment method of N-p-aminobenzoyl-L-glutamic acid medical intermediate wastewater, and belongs to the technical field of pharmaceutical wastewater treatment. The treatmentmethod comprises the following steps: 1, adjusting pH: adjusting the pH of raw wastewater to 2-6 to obtain wastewater A; 2, performing Fenton oxidation: adding a mixture of water-soluble ferrous saltand an aqueous hydrogen peroxide solution or the aqueous hydrogen peroxide solution into the wastewater A to obtain wastewater B, performing solid-liquid separation to obtain a supernatant of the wastewater B; 3, performing coagulating sedimentation: adjusting the pH of the wastewater B to 7.5-9, adding a flocculant to obtain a flocculent precipitate and wastewater C; and 4, performing electrocatalytic oxidation: sending the wastewater C to an electrocatalytic oxidation system and discharging water to obtain purified water. By the treatment method, treatment of the wastewater is achieved andthe wastewater is discharged after standards are met.

The invention discloses a fresh-keeping method of living Chinese toon buds through salicylic acid solution treatment. The method comprises the following specific steps: washing Chinese toon buds withrunning water, airing the buds in a shade place, soaking the buds in 84 disinfectant for half an hour, taking out the buds, and airing the buds in a shade place; and selecting 2000ml of 10-100 [mu]mol / L salicylic acid solution, soaking sterilized Chinese toon buds in the solution for 20 minutes, taking out and airing the Chinese toon buds, and storing the treated Chinese toon buds in a refrigerator at 4-8 DEG C. After being picked, the tender Chinese toon buds can age and brown along with the storage time. After being treated by salicylic acid, the content of vitamin C in the Chinese toon decreases, the content of anthocyanin firstly rises and then decreases, and the salicylic acid reduces the damage of the Chinese toon in the storage process, well maintains the nutritional quality of theChinese toon, and controls the increase of the content of malondialdehyde and hydrogen peroxide.

The invention discloses pseudoalteromonas sp.B3 and application thereof to biological oxidation of L-amino acid. During the application of the pseudoalteromonas sp.B3, an enzyme cultured by fermenting the pseudoalteromonas sp.B3 is taken as an enzyme source, the L-amino acid is taken as a substrate, and the enzyme source and the substrate react for 0.5-2h at a temperature of 25-50 DEG C and the pH value of 6-8 so as to oxidize the L-amino acid and release hydrogen peroxide. The invention provides a novel microbial strain for producing L-amino acid oxidase, and the strain can be use for biologically oxidizing the L-amino acid, has the characteristics of wide substrate spectrum, mild reaction, environment friendliness and the like, and has a wide application prospect in the aspects of quantitative analysis of the L-amino acid, splitting of the DL-amino acid and the like.

The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme multiplication method, an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, pyruvic acid, hydrogen peroxide, an amino acid oxidase, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the speed of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.

The invention provides a method for quantitatively detecting the activity of L-amino acid oxidase by utilizing a xylenol orange agar flat plate. The method comprises the following steps of: punching small holes with the diameter of 4-10mm in the xylenol orange agar flat plate by using a hole puncher, then adding a solution to be detected into the small holes, standing at room temperature for 20-100min, determining the diameter of a purple-red circle, determining the concentration of hydrogen peroxide in the solution to be determined by a standard straight line made according to a log value of the concentration of the hydrogen peroxide and the diameter of the purple-red circle, and further determining the activity of the L-amino acid oxidase, wherein the solution to be detected takes an enzyme solution to be detected of the L-amino acid oxidase as an enzyme source, 0.2-20mmol / L of L-leucine is taken as a substrate, the reaction is performed for 0.5-2h under the conditions that the temperature is 25-50 DEG C and the pH value is 5-9, then the hydrogen peroxide is produced, and an obtained reaction solution is diluted to obtain the solution to be detected. The method provided by the invention has the characteristics of very high sensitivity, low cost, simplicity and convenience in operation, good stability, wide range of application, environmental friendliness and the like.

The invention relates to the technical field of water reducing agents, in particular to an efficient retarding water reducing agent for highway concrete and a preparation method thereof. The efficient retarding and water reducing agent for the highway concrete is prepared from the following components: isobutene alcohol polyoxyethylene ether, maleic anhydride, alpha methacrylic acid, allyl trimethyl ammonium chloride, ammonium persulfate, ascorbic acid, calcium lignosulphonate, hydrogen peroxide, an acid regulator, an alkali regulator and distilled water. Through molecular design, much -COO- is introduced into molecules of the polycarboxylate superplasticizer, so that the retarding effect of the superplasticizer is enhanced, and alpha-methacrylic acid is used for replacing acrylic acid, so that the self-polymerization degree of a monomer is reduced. By adding the reducing agent ascorbic acid, the temperature of the reaction system is reduced from about 80 DEG C to room temperature, and the energy consumption is reduced under the condition that the performance is basically unchanged. By adding the modified calcium lignosulphonate, the synthesis cost of the polycarboxylate superplasticizer is greatly reduced under the condition of small performance loss.

The invention discloses a preparation method of salicylamide derivatives shown in the formula 1. The preparation method comprises the following steps that 2,5-dimethoxyaniline is dissolved in an organic solvent and reacts with ethyl acetate of O-alkylacylsalicyloyl chlorine to produce N-(2-alkylacyloxyformoxy)-2,5-dimethoxyaniline; the N-(2-alkylacyloxyformoxy)-2,5-dimethoxyaniline reacts with R3OH in the presence of iodobenzene diacetate to produce 3-(O-alkylacylsalicyloylamino)-4,4-dialkoxy-2,5-cyclohexadienone; in an organic solvent (such as tetrahydrofuran, methanol and dimethyl formamide and especially such as dimethyl formamide), the 3-(O-alkylacylsalicyloylamino)-4,4-dialkoxy-2,5-cyclohexadienone undergoes a reaction in the presence of a hydrogen peroxide aqueous solution and an inorganic base to produce 5,6-epoxy-4,4-dialkoxy-3-salicyloylamido-2-cyclohexenone; the 5,6-epoxy-4,4-dialkoxy-3-salicyloylamido-2-cyclohexenone undergoes a reaction in the presence of a boron trifluoride ether complex to produce 5,6-epoxy-2-salicyloylamido-2-cyclohexenyl-1,4-dione; and the 5,6-epoxy-2-salicyloylamido-2-cyclohexenyl-1,4-dione is reduced into 5,6-epoxy-4-hydroxy-3-salicyloylamido-2-cyclohexenone (DHM2EQ) shown in the formula (1). The invention also relates to a preparation method of the 5,6-epoxy-4-hydroxy-3-salicyloylamido-2-cyclohexenone.

The invention relates to a method for preparing urea peroxide containing organic titanium by a recrystallization process. The method is characterized in that: new metatitanic acid precipitate of citric acid is prepared by neutralizing an iced solution of titanium salt by using alkali; the new metatitanic acid precipitate instead of titanium chloride and sulfate is used as raw materials for synthesizing citricacide-titatnium chelate; the citricacide-titatnium chelate is synthesized by a microwave hot melting chelation process by using the new metatitanic acid precipitate of citric acid as a titanium source and citric acid as an acid solvent and chelant; and the novel urea peroxide fertilizer containing the organic titanium is prepared by the steps of uniformly mixing hydrogen peroxide, acid citricacide-titatnium chelate and urea in a ratio, performing addition reaction on a mixture at the constant temperature of 60 DEG C, and recrystallizing at the temperature of less than or equal to 5 DEG C. The titanium element can strongly promote plant to absorb and convey nitrogen, phosphorus, potassium, other secondary elements and trace elements; the citricacide-titatnium chelate is reacted with hydrogen peroxide easily to form a titanium peroxide compound with high stability; and the citricacide-titatnium chelate can be used as stabilizer of urea peroxide, and synergist of urea peroxide nitrogenous fertilizer.

The invention relates to a preparation method for high purity catalpic acid and series products like ester and salt thereof. According to the invention, catalpa seed is used as a raw material, and catalpic triglyceride is extracted; catalpic triglyceride undergoes saponification and acidification so as to obtain catalpic acid; catalpic acid is esterified so as to obtain catalpic acid ester, or catalpic acid ester is prepared by directly esterifying catalpic triglyceride; obtained catalpic acid and catalpic acid ester are subjected to separation through two-step freezing crystallization so as to obtain catalpic acid and catalpic acid ester with purity of 95 to 98%; and n catalpic acid and catalpic acid ester with purity of 95 to 98% undergo saponification so as to obtain catalpic acid salt with purity of 95 to 98%. Compared with the prior art, the method provided by the invention has the advantages of simple process, low cost, high purity of obtained catalpic acid and ester and salt thereof and wide application prospects.

A method of treating and preventing type 2 diabetes and obesity an animal, including mammals and humans, in which a therapeutically effective amount of catalpic acid to the animal is administered orally or parentally.

A method of treating and preventing dyslipidemia, cardiovascular disease, type 2 diabetes, obesity and other diseases associated with the Metabolic Syndrome and of increasing the expression of genes that promote lipid oxidation in an animal, including mammals and humans, in which a therapeutically effective amount of catalpic acid is administered orally or parenterally to the animal.

The invention relates to a preparation method of a polyhydroxyl-modified soybean phosphatide fatliquoring agent. The soybean phosphatide fatliquoring agent is widely used for fatliquoring at present, while the traditional fatliquoring agent is relatively poor in water solubility and is also not good in filling property. The preparation method comprises the steps of placing 100 parts by mass of soybean phosphatide into a three-neck flask, then, adding 2-2.5 parts by mass of allyl glycidyl ether, heating to 40 DEG C, and reacting at the constant temperature for 2h; adding 5.4-7.7 parts by mass of formic acid into the system, dropwise adding 25-40 parts by mass of hydrogen peroxide within 1h by taking 0.5-1 part by mass of concentrated sulfuric acid as a catalyst, and reacting for 8-10h; heating the system to 75 DEG C, adding 40-65 parts by mass of sodium hydrogen sulfite, and reacting for 3h; and regulating the pH value of the system to 7.5-8 by using 40% NaOH, adding 60-DEG C hot water to regulate the solid content of the system to 60%, and stirring for 30min to obtain the improved fatliquoring agent. The soybean phosphatide fatliquoring agent prepared by using the method disclosed by the invention has better hydrophilicity.

The invention discloses a biosynthesizing method of imidazole-4-acetic acid. The biosynthesizing method comprises the following steps of using L-histidine as a substrate, and performing deaminizing reaction under the catalyzing action of membrane-linked L-amino acid deaminases, so as to obtain reaction liquid containing an intermediate product, namely 3-(4-imidazole)-2-oxo propionic acid; adding a hydrogen peroxide solution into the reaction liquid, and reacting, so as to obtain the imidazole-4-acetic acid. The biosynthesizing method has the characteristics that the steps are simple, the reaction conditions are mild, the sources of raw materials are wide, the preparation of a biological catalyst is easy, the requirement on equipment is low, and the conversion rate of the substrate is high; the organic solvent and the toxic reagent are not needed; compared with the existing chemical method, the efficiency is high, the cost is low, and the environment-friendly effect is realized.

The invention provides a biocatalysis method for preparing pyruvic acid from L-alanine. The method comprises the steps of: carrying out permeability treatment on Alcaligens faecalisl.1799 cells; catalyzing oxidative deamination of L-alanine by utilizing L-amino acid oxidase in the cells in the presence of air to form pyruvic acid, ammonia and hydrogen peroxide; catalyzing the hydrogen peroxide formed in the reaction by utilizing catalase in the cells to realize pyruvic acid accumulation without producing by-products. The method provided by the invention has the advantages of low cost, high product yield and purity and environment friendliness, and is suitable for industrial production of pyruvic acid.

Disclosed is a method for producing an alkanolamine salt of an a-sulfo fatty acid alkyl ester, comprising: a step of sulfonating a fatty acid alkyl ester to obtain a sulfonated compound; a step of reacting an alcohol and hydrogen peroxide with the sulfonated compound to obtain a reaction product; a step of adding an aqueous alkanolamine solution to the reaction product to obtain a neutralized product having a pH of 2 to 6.5; and a step of concentrating the neutralized product to obtain an aqueous paste containing an alkanolamine salt of an a-sulfo fatty acid alkyl ester. Accordingly, the deterioration of color tone at the time of concentration when preparing an aqueous paste of an alkanolamine salt of an a-sulfo fatty acid alkyl ester can be prevented.

The invention relates to the technical field of plant extraction and provides a method for extracting a pollen amino acid liquid from rose pollen. The method comprises steps as follows: step one, the rose pollen is processed, and then boiling water is added for evaporation; step two, a hydrogen dioxide solution is added; step three, water is added, washing is performed, then the mixed solution is left to stand, primary precipitate is obtained through centrifugation, and after the primary precipitate is washed for 3-4 times, final precipitate is obtained; step four, a phosphate buffer solution, cellulase and lysozyme are added to the final precipitate, the mixture is subjected to enzymolysis and then is boiled and centrifuged, and a supernatant is taken as the pollen amino acid liquid. The extraction process does not involve metal grinding, avoids noise and strong corrosive substances and eliminates heavy metal pollution and noise pollution of products; the pollen amino acid liquid contains pollen active substances, is free of chemical agent residues, removes sensitization effect of residual pollen wall and is suitable for being taken as a raw material of health care products and cosmetics; meanwhile, rose liquid waste obtained after essential oil extraction is recycled, so that the rose pollen resource is effectively utilized, and the production cost is greatly reduced.