Method for preparing beta-alanine from maleic acid through multi-enzyme coupling

Abstract

The invention specifically relates to a method for preparing beta-alanine from maleic acid through multi-enzyme coupling, belonging to the field of biotechnology. The method comprises the following steps: adjusting the pH value of an aqueous maleic acid solution with a certain concentration to 6 to 9 by using ammonia water; then separately adding bacterial cells respectively with maleate isomerase activity, aspartase activity and aspartate-alpha-decarboxylase activity or crude liquid of the three enzymes; carrying out enzymatic reaction at 30 to 50 DEG C; and separating and converting products by using isoelectric-point crystallization or a combination of isoelectric-point crystallization and ion exchange resin so as to obtain high-purity beta-alanine. The method provided by the invention uses maleic acid as a substrate for preparation of beta-alanine via multi-enzyme coupling and has the advantages of wide sources of raw materials, low price, simple operation, low production cost, suitability for large-scale industrial production, etc.

Description

[0001] 1. Technical field [0002] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing beta-alanine by using maleic acid as a raw material through multi-enzyme coupling. [0003] 2. Background technology [0004] β-alanine is a precursor for the synthesis of pantothenic acid, a type of B vitamin that forms part of coenzyme A and is important for the exchange of endogenous metabolic energy in various tissues. With the gradual deepening of the understanding of β-alanine, β-alanine is widely used in the fields of medicine, food, cosmetics and feed, and the market demand for β-alanine is also growing. Therefore, β-alanine Acid has a wide range of application value and development prospects. [0005] According to literature reports, the current preparation methods of β-alanine mainly include chemical synthesis and biological enzymatic methods. [0006] 1. Chemical synthesis method [0007] Chemical synthesis methods include acr...

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Problems solved by technology

Wherein the acrylonitrile method is that acrylonitrile and ammonia react in diphenylamine and tert-butanol solution to produce β-aminopropionitrile, which is then obtained by alkaline hydrolysis. The disadvantage of this method is that there are more than 40% NaCl and impurities in the product. Requires repeated purification with low yield; the β-aminopropionitrile method is to react β-aminopropionitrile with barium hydroxide to generate β-aminopropionate barium and nitrogen, and introduce CO 2 , the barium salt is precipitated to produce β-alanine, and the barium ion is removed by resin. The disadvantage of this method is that the production cost is high; the succinimide degradation method is that succinimide generates β-amino in alkaline sodium chlorate solution Propionic acid, the pH of the reaction solution is adjusted with hydrochloric acid, the inorganic salt is precipitated with 3 times the amount of 95% ethanol, the filtrate is diluted with 4 times the amount of distilled water, the ion exchange resin is separated and decolorized, concentrated and crystallized, because this method requires a large amount of ethanol, the production High costs and high safety requirements for production sites

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