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Preparation method of probiotic inoculant

A technology for bacteria and probiotics, applied in the field of preparation of probiotic bacteria, can solve the problems of poor synergy, affecting the curative effect or health care effect of probiotic products, and decreasing the number of viable bacteria, so as to increase the body's appetite and improve the health of the body. Enteric-coated, the effect of increasing the absorption of the digestive tract

Inactive Publication Date: 2018-03-06
CHANGZHOU AMANTE CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 4. The technical problem to be solved by the present invention: for the current probiotic preparation, its synergistic effect in the intestinal microenvironment is not good, and when it is taken orally, it is easily affected by gastric acid and bile. will be greatly reduced, thereby affecting the curative effect or health care effect of probiotic products, and a method for preparing probiotic bacterial agents is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] A preparation method of a probiotic bacterial agent, comprising the steps of:

[0032] (1) Add kimchi and yogurt to normal saline at a mass ratio of 1:2:9, shake at 35-37°C and 200 r / min for 1-2 h, inoculate on a separation agar plate, and culture anaerobically at 35-37°C for 2 ~3 days, pick the colony with the largest diameter and repeat the streak culture 2~3 times to obtain the purified cultured strain, inoculate the purified cultured strain into the seed medium at a mass ratio of 1:50, and incubate at 30~33°C for 18~24h , inserting the culture into the fermentation medium with an inoculation amount of 5% by mass fraction, culturing at 30-35°C for 2-3 days, drying, pulverizing and passing through a 100-mesh sieve to obtain a fermented product powder, which is set aside;

[0033] (2) Take the leaves and add 50% ethanol solution at a mass ratio of 1:20 to mix, heat up to 50-60°C for ultrasonic extraction for 1-2 hours, filter with suction, take the filtrate, rotate it ...

Embodiment 1

[0037] Yogurt and pickles: buy from Tesco.

[0038] Separation liquid medium: in parts by mass, take 5 parts of beef extract, 10 parts of peptone, 3 parts of sodium chloride, 1000 parts of distilled water, pH 7.2, and autoclave at 121°C for 15-20 minutes.

[0039] Separation agar medium: other components in the separation liquid medium remain unchanged, and then add agar with a mass of 1.8% of the separation liquid medium to obtain the product.

[0040] Seed medium: in parts by mass, take 10 parts of glucose, 8 parts of yeast powder, 3 parts of peptone, 1 part of sodium acetate, 0.02 part of magnesium sulfate, 0.001 part of manganese sulfate, 0.001 part of ferrous sulfate, 0.001 part of sodium chloride 1,000 parts of distilled water, pH 6.8, autoclaved at 121°C for 15 minutes.

[0041] Fermentation medium: the other components in the seed medium remain unchanged, and 1% of the mass of the seed medium is added with L-sodium glutamate to obtain it.

[0042] Mixed protective ag...

Embodiment 2

[0050] Yogurt and pickles: buy from Tesco.

[0051] Separation liquid medium: in parts by mass, take 7 parts of beef extract, 11 parts of peptone, 4 parts of sodium chloride, 1000 parts of distilled water, pH 7.3, and autoclave at 121°C for 17 minutes.

[0052] Separation agar medium: other components in the separation liquid medium remain unchanged, and then add agar with a mass of 1.9% of the separation liquid medium to obtain the product.

[0053] Seed medium: in parts by mass, take 12 parts of glucose, 9 parts of yeast powder, 4 parts of peptone, 2 parts of sodium acetate, 0.03 part of magnesium sulfate, 0.002 part of manganese sulfate, 0.003 part of ferrous sulfate, 0.005 part of sodium chloride 1,000 parts of distilled water, pH 6.9, autoclaved at 121°C for 17 minutes.

[0054] Fermentation medium: the other components in the seed medium remain unchanged, and 1% of the mass of the seed medium is added with L-sodium glutamate to obtain it.

[0055] Mixed protective agen...

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Abstract

The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method of a probiotic inoculant. In the invention, a high-yield gamma-aminobutyric acid inoculant is obtained from the separation and screening of yogurt and pickled vegetables. The gamma-aminobutyric acid inoculant can increase organism appetites, promote growth, regulate intestinal flora and improve the absorlption effect of alimentary canals, and has health-care effects of promoting nutrient absorption and promoting growth. In the invention, flavonoids in paper mulberry leaves and saponin substances in stauntonia chinensis are extracted, and the flavonoids and the saponin substances are important growth factors for probiotics, and thorough the cooperation with the probiotics, the flavonoids and the saponin substances can improve the survival rate of the probiotics, prolong the action time of the probiotics, lead the probiotics to reach the body in the largest quantity, and stimulate the colonization and growth of endogenous and exogenous probiotics of intestinal tracts, and can also enhance the metabolic activity of the probiotics, enhance the immune functions of theorganisms, directly antagonize pathogens, and stimulate the production of endogenous antibiotic substances of the organisms.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a preparation method of a probiotic agent. Background technique [0002] Probiotics refer to live bacterial preparations and their metabolites that can improve the ecological balance of the host’s intestinal flora, improve the health level and health status of the host. Probiotics can supplement the reduced or lacking normal microorganisms in the intestine in terms of quantity and type, adjust or maintain the balance of microorganisms in the intestine, enhance the immune function of the body, and promote the consumption and absorption of nutrients, so as to achieve the purpose of disease prevention and treatment. Probiotics include Bacillus subtilis, Streptococcus faecalis, Bifidobacterium, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Enterococcus, Leuconostoc, Pediococcus, Bacillus cereus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135C12N1/00
CPCA23L33/135A23V2002/00C12N1/00A23V2200/32
Inventor 吴昌财郑薇林凡
Owner CHANGZHOU AMANTE CHEM CO LTD
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