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Co-production fermentation method for astaxanthin and mannan and application of astaxanthin and mannan

A technology of mannan and fermentation method, which is applied in the field of preparation of astaxanthin and mannan, and achieves the effects of breaking walls, high economic value and wide application range

Pending Publication Date: 2018-05-01
杭州皇冠农业生物工程技术研究中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant research on the co-production and fermentation of astaxanthin and mannan

Method used

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  • Co-production fermentation method for astaxanthin and mannan and application of astaxanthin and mannan
  • Co-production fermentation method for astaxanthin and mannan and application of astaxanthin and mannan
  • Co-production fermentation method for astaxanthin and mannan and application of astaxanthin and mannan

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Experimental program
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Embodiment 1

[0036] The co-production fermentation method of astaxanthin and mannan includes: mutagenesis, preparation of protoplasts, genome rearrangement, acquisition of astaxanthin, and extraction of mannan, specifically including the following steps:

[0037] Mutagenesis: Dilute the activated and cultured Phaffia rhodozyma suspension to 1×10 6 cells / mL, take 5mL of bacterial suspension in a Petri dish with a magnetic rotor, add 300μL of 10% LiCl aqueous solution, mix well, open the cover and irradiate with ultraviolet rays. Plates were cultured in a constant temperature incubator at 20°C for 4 days; at the same time as LiCl-ultraviolet compound mutagenesis, the concentration of the prepared bacterial suspension was adjusted to 1×10 6 cells / mL, take the bacterial suspension in a sterile test tube, and send it to the cobalt room of Zhejiang Academy of Agricultural Sciences for 60 Co-γ-ray mutagenesis; lithium chloride is an alkali metal halogenating agent, which has no mutagenic effect ...

Embodiment 2

[0045] 1) Determination of astaxanthin:

[0046] Take 5 mL of fermentation broth and centrifuge at 5000 rpm for 10 minutes, wash twice with deionized water, collect the bacteria, blot the water droplets on the tube wall with filter paper, add 1 mL of dimethyl sulfoxide preheated at 55°C, shake and suspend, add 4 mL of acetone, and vortex 20-30s, 4°C, centrifuge at 5000rmp for 10min, measure the OD of the supernatant with a spectrophotometer 474 . If the sludge is still colored, it can be repeatedly extracted until it becomes white. The formula for calculating the astaxanthin content is as follows:

[0047]

[0048] Where: OD 474 ——the absorbance of the extract at a wavelength of 474nm; V l ——the total volume of organic solvent, mL; V 2 - volume of fermentation broth, mL; W - dry weight of cells, g; 0.21 - extinction coefficient of organic solvent;

[0049] 2) Determination of cell biomass:

[0050] Take 5 mL of bacterial suspension, centrifuge at 5000 rpm for 10 minut...

Embodiment 3

[0052] Mutation of Phaffia rhodozyma cells:

[0053] Dilute the activated and cultured Phaffia rhodozyme bacterial suspension to 1.2×10 6 cells / mL, take 10mL of bacterial suspension in a petri dish with a magnetic rotor, add 350 μL of 12% LiCl aqueous solution, mix well, open the cover and irradiate with ultraviolet rays. plate, cultured in a constant temperature incubator at 22°C for 5 days; at the same time of LiCl-ultraviolet compound mutagenesis, the concentration of the prepared bacterial suspension was adjusted to 1.2×10 6 cells / mL, take the bacterial suspension in a sterile test tube, and send it to the cobalt room of Zhejiang Academy of Agricultural Sciences for 60 Co-γ-ray mutagenesis; lithium chloride is an alkali metal halogenating agent, which has no mutagenic effect itself, but it is combined with ultraviolet rays, and within a certain concentration range, it is conducive to the occurrence of positive mutations in bacteria; the lethality of bacteria is selected ...

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Abstract

The invention discloses a co-production fermentation method for astaxanthin and mannan and application of the astaxanthin and the mannan. The co-production fermentation method for the astaxanthin andthe mannan comprises: mutagenesis, preparation of protoplast, genome rearrangement, obtainment of the astaxanthin and extraction of the mannan. The astaxanthin is applied as an antioxidant additive infeeds, food and cosmetics; and the mannan can be applied in adhesives, fixatives, feed additives, cosmetic additives, embedding materials in wastewater treatment, dust preventives, chromatographic packing materials and food preservation. The beneficial effects are as follows: high-yield phaffia rhodozyma strains are obtained by a dual induction method, a strain for the high yield of the astaxanthin is screened out by a genome rearrangement and cell fusion method, so that a genetically stable phaffia rhodozyma strain is obtained, and the astaxanthin and the mannan are obtained by co-productionfermentation; the application ranges of the astaxanthin and the mannan are wide, and economic value is high.

Description

technical field [0001] The invention relates to the field of preparation of astaxanthin and mannan, in particular to a co-production fermentation method of astaxanthin and mannan and its application. technical background [0002] Astaxanthin is 3,3′-dihydroxy-4,4′-diketone-β,β′-carotene, which is an unsaturated terpene compound with the molecular formula C 40 h 52 o 4 , There are two β-ionone rings and 11 conjugated double bonds in the molecular structure. Astaxanthin is widely found in nature, such as most crustaceans and salmonids, leaves, flowers, fruits of plants, and feathers of flamingos. It is a high value-added ketone carotenoid. It has bright colors and can be used as feed to improve the muscle color of fish and poultry and promote growth. Astaxanthin has a strong ability to quench singlet oxygen and scavenge oxygen free radicals in organisms, so it has a wide range of applications in anti-radiation, anti-oxidation, anti-tumor, aquaculture, cosmetics, health pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/04C12N13/00C12P23/00C12P19/04C07C403/24C08B37/02C12R1/645
CPCC07C403/24C08B37/0003C08B37/0087C12N13/00C12N15/04C12P19/04C12P23/00
Inventor 胡向东叶茂胡伟卿
Owner 杭州皇冠农业生物工程技术研究中心有限公司
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