A new strain of Bacillus coagulans and its probiotics and feed

A technology of Bacillus coagulans and composite microecology, which is applied in the direction of microorganisms, microorganisms, animal feed, etc., can solve the problems of heavy losses of farmers, nitrite poisoning, and applications that have not been reported, so as to improve growth performance and reduce the number of bait , the effect of improving the survival rate

Active Publication Date: 2021-09-28
BEIJING DABEINONG TECH GRP CO LTD +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nitrite poisoning has always been another difficult problem encountered in the breeding process, which often brings heavy losses to farmers
Most of the biodegradation of nitrite requires the participation of oxygen, and the current high-density farming results in insufficient oxygen in the pond. We need to develop new products that can degrade nitrite under low oxygen conditions.
[0006] At present, there is no product using Bacillus coagulans as a streptococcal antagonist in China, and the application of this bacteria in tilapia feed has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A new strain of Bacillus coagulans and its probiotics and feed
  • A new strain of Bacillus coagulans and its probiotics and feed
  • A new strain of Bacillus coagulans and its probiotics and feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of Bacillus coagulans CGMCC No.12196

[0035]1. Isolation of bacterial strains: Weigh 1 g of soil sample, place it in 100 ml of sterilized modified MRS (MRS medium with 10 g / L of α-methyl glucoside and 1 g / L of potassium sorbate) liquid medium, shake for 10 min, Place in a 42°C incubator for enrichment culture for 4 days, shaking once a day. After the enrichment, take 2mL of the liquid and put it in a water bath at 90°C for 10 minutes, and then perform a 10-fold serial dilution. Select three dilutions of 10 times, 100 times and 1000 times, pipette 100 μl into the MRS plate, each dilution is repeated three times, spread evenly with a sterile applicator, and incubate at 42°C for 24 hours. Pick a single colony for Gram staining and microscopic examination, and isolate a Gram-positive, rod-shaped, spore-forming bacterium, streak and purify it three times on the plate, and take a single colony for preservation. The bacterium appears a...

Embodiment 2

[0039] Example 2 Hemolytic test of Bacillus coagulans CGMCC No.12196

[0040] The isolated Bacillus coagulans CGMCC No. 12196 was streak-inoculated onto fresh blood culture medium and cultured at 37°C for 24 hours. After observation, no hemolytic ring was found. It shows that the strain has no hemolytic activity and is a relatively safe strain.

Embodiment 3

[0041] Example 3 Bacteriostatic ability test of Bacillus coagulans CGMCC No.12196

[0042] Melt 100 mL of nutrient broth agar (containing 1% glucose) in the Erlenmeyer flask and cool to 45°C. Add 100ul of Streptococcus iniae and Streptococcus agalactiae cultivated overnight, mix well, pour into sterile plate, pour 15-20ml into each plate. Two Oxford cups are pre-placed on each plate, and the Oxford cups are taken out after the agar solidifies, and agar holes are formed on the plate. Add 150 μL of the bacteria solution to be tested to the agar well, set up 3 repetitions, cover the dish cover, carefully move it to a 37°C incubator, put the plate upright, and culture it statically. At the same time, it was compared with two commercially available Bacillus coagulans powders. After culturing for 20 hours, open the lid of the dish and measure the diameter of the inhibition zone with a caliper. Through experiments, it was found that Bacillus coagulans CGMCC No.12196 had significan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a strain of Bacillus coagulans antagonizing streptococcus and its application. The bacterial strain has been preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC No.12196. The bacterial strain is made into bacteria powder, which can be added to animal feed, and can also be used as a microbial preparation for antagonizing streptococci. Feeding tilapia with the feed containing the bacterial strain can effectively prevent and treat streptococcal disease. Mix the bacteria with Bacillus subtilis and Lactobacillus johnsonii to make a compound microecological preparation, which can improve the growth performance of tilapia, increase the activity of liver superoxide dismutase, catalase and lysozyme in serum . In addition, the fermented liquid of the bacteria can also be used as a culture environment improver. Sprinkling the fermented liquid of the above bacteria into the tilapia culture pond can reduce the number of streptococci in the culture water, and can also remove the streptococci in the water under low oxygen conditions. nitrite.

Description

technical field [0001] The invention belongs to the field of feed microecology, and in particular relates to a new strain of bacillus coagulans, a microecological preparation and feed. Background technique [0002] China is the largest tilapia producer in the world, ranking first in the world in output, export volume and export value. Tilapia is the most internationally competitive freshwater aquaculture species in China, and it is also the species with the most industrialized development conditions. It has become a pillar industry in southern China and has strong development advantages in the country. However, the diseases of tilapia caused by Streptococcus agalactiae and Streptococcus iniae are easy to break out in summer and autumn. 25% to 80%, or even higher, has caused great economic losses to the tilapia aquaculture industry, and there is a growing trend. [0003] At present, in tilapia farming and production, people mainly use antibiotic chemical drugs to prevent an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C02F3/34C12R1/07C12R1/125C12R1/225
CPCC02F3/34C12N1/20C12N1/205C12R2001/07
Inventor 汪攀易敢峰孙自博朱传忠付维来刘婷韩科厅陈丽仙
Owner BEIJING DABEINONG TECH GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap