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Extraction process and application of flavonoids from fenugreek

A technology of weed grass flavonoids and extraction process, which is applied in the antibacterial application, extraction of weed grass flavonoids, antibacterial and anti-oxidation fields, can solve the problems that the research of weed grass flavonoids has not yet been carried out, and achieve accurate and reliable extraction process conditions and strong antibacterial Strong activity and antibacterial activity

Active Publication Date: 2020-07-17
上海铮信生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on the flavonoids of weed grass have not yet been carried out

Method used

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  • Extraction process and application of flavonoids from fenugreek
  • Extraction process and application of flavonoids from fenugreek
  • Extraction process and application of flavonoids from fenugreek

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Extraction process optimization and component analysis of weed grass flavonoids

[0035] Step 1: Take the weed grass collected in late December, wash it with clean water, dry the surface water at room temperature, dry it at 35°C and crush it, and pass it through a 60-mesh sieve for later use;

[0036] Step 2: Add petroleum ether with a liquid-solid ratio of 15:1 to the weed grass powder obtained in step 1 to extract and remove chlorophyll.

[0037] Step 3: Add extractants with liquid-solid mL / g ratios of 15:1, 20:1, 25:1, 30:1, 35:1, 40:1 and 45:1 to the weed grass powder obtained in step 2 agent; ethanol volume fractions were 40%, 50%, 60%, 70%, 80% and 90%; extraction temperatures were 40°C, 50°C, 60°C, 70°C, 80°C; Under the conditions of 200W, 300W, 400W, 500W and 600W, ultrasonically extract for 10min, 20min, 30min, 40min, 50min, then use a vacuum filter to filter, spin evaporate and concentrate, and then freeze-dry to obtain the crude flavonoids of the ...

Embodiment 2

[0069] Embodiment 2: Antibacterial effect of weed grass flavonoids

[0070] Step 1: Preparation of weed grass flavonoids

[0071] Get 1000g of dry grass sage powder, use the snail flavonoids obtained in Example 1 to optimize the extraction process (ultrasonic power is 315W, ultrasonic extraction time is 23min, liquid-solid ratio mL / g is 39.8:1, ethanol volume fraction 70.2%, ultrasonic The extraction temperature was 70°C, and the flavonoids were prepared, separated and purified. After the flavonoids were freeze-dried, 21.9 g of light yellow flavones were obtained, and the yield of the flavonoids was 2.19%.

[0072] Step 2: Antibacterial effect of weed grass flavonoids

[0073] (1) Tested strains:

[0074] Escherichia coli, Staphylococcus aureus, pathogenic bacteria of pear ringworm, Penicillium grisea (all the above strains were provided by Microbiology Laboratory of Suzhou University of Science and Technology).

[0075] (2) Medium:

[0076] Beef extract-peptone medium: 3g...

Embodiment 3

[0090] Embodiment 3: Antioxidative effect of weed grass flavonoids

[0091] (1) Take 3.9mL of the prepared DPPH reagent and put it in a clean test tube, then immediately add 0.1mL of different concentrations of weed grass flavonoid solutions dissolved in DMSO, shake well, and store at room temperature (about 25°C) and avoid React under light conditions for 30 minutes, and then measure the absorbance value at a wavelength of 515nm to obtain the absorbance value A1 of the weed grass flavonoid sample. In addition, take 3.9mL of DPPH reagent, add 0.1mL of solvent for dissolving the sample, shake it well and quickly, react at room temperature (about 25°C) and avoid light for 30min, and then measure the absorbance value at a wavelength of 515nm to obtain the absorbance value of A blank A2 .

[0092] DPPH clearance %=[(A 2 -A 1 ) / A 2 ]×100%

[0093] (2) The results of antibacterial activity determination are shown in Table 5.

[0094] Table 5 The scavenging effect of the flavon...

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Abstract

The invention provides an extraction technology of arthraxon hispidus flavonoid and application thereof. The extraction technology comprises the following steps: taking an aboveground part of arthraxon hispidus, washing, airing, drying, crushing and then sieving; extracting chlorophyll from the arthraxon hispidus by using petroleum ether as an extractant, discarding a chlorophyll-containing supernatant, airing obtained arthraxon hispidus powder at room temperature, then performing ultrasonic extraction, performing suction filtration, then performing rotary evaporation and concentration, performing column chromatography separation and performing freeze drying to obtain the arthraxon hispidus flavonoid. Through a single-factor experiment and a BOX-Behnken central composite response surface test, the extraction technology of the arthraxon hispidus flavonoid is designed and optimized, and the optimal extraction technology is screened. The extraction technology of the arthraxon hispidus flavonoid and application thereof are researched for the first time, and the product extracting rate and the product purity are high. Research on the antioxidant and bacteriostatic effects of the arthraxon hispidus flavonoid shows that the arthraxon hispidus flavonoid has certain anti-oxidation activity and has relatively strong bacteriostatic and bactericidal activity for escherichia coli, staphylococcus aureus and physalospora piricola nose, so that the arthraxon hispidus flavonoid has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of extraction and separation, and relates to an extraction process and application of flavonoids, in particular to an extraction method of weed grass flavonoids, and the anti-oxidation, antibacterial and bacteriostatic applications of the flavonoids. Background technique [0002] Weed grass [ Arthraxon hispidus (Thunb.) Makino] is an annual grass plant, aliased as Lu bamboo, Wang Chu, horse ear grass, yellow grass and so on. Grass stalks are thin and hairless, the base is inclined, 30-45cm high, and the branches are knotty. Leaf sheaths shorter than internodes, with short hard warty hairs; ligule membranous, with ciliates at the margin; leaf blade ovate-lanceolate, 2-4cm long, 8-15mm wide, glabrous except for ciliates on the lower margin, The flowering and fruiting period is August-November. There is such a description in "Shen Nong's Materia Medica", "The leaves are thin like bamboo, and the stems are also round a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K36/899A61K8/9794
CPCA23L33/105A23V2002/00A61K8/97A61K36/899A61K2236/333A61K2236/51A61K2236/55A61Q17/005A61Q19/08A23V2200/30A23V2250/2116Y02A50/30
Inventor 王桃云冀小草邱业先李良智扶教龙胡翠英严立石陈宏伟顾华杰钱玮
Owner 上海铮信生物科技股份有限公司
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