Preparation method of microbial feed additive

A microbial feed and additive technology, applied in the field of feed additives, can solve the problems of reducing biological activity, easy inactivation during processing, transportation and storage, and achieve the effects of inhibiting growth, enhancing non-specific immunity, and strong resistance

Inactive Publication Date: 2018-12-21
FOSHAN SENANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: Aiming at the problem that most of the existing microbial feed additive components are living bacteria, which are easily inactivated during feed processing, transportation and storage, thereby reducing their biological activity, a microbial feed additive is provided preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] The liquid medium is composed of 60.0% deionized water, 20.0% peptone, 14.0% beef extract, 4% agar powder, 0.90% sodium bicarbonate, and 1.10% sodium carbonate in terms of mass percentage, and it is sterilized at 120°C for 15 minutes; After Bacillus subtilis and Lactobacillus were inoculated into the liquid medium, they were incubated at a temperature of 30°C with an inoculum size of 3% and a stirring speed of 180r / min for 1 day to obtain a bacteria-containing solution. / min, the vacuum degree is 3mbar, and the pumping rate is 26L / min, and it is centrifuged and concentrated for 20min to obtain the bacterium; the starch and xanthan gum are mixed evenly according to the mass ratio of 1:3 to obtain a mixed powder, and the mass ratio is 1:1. Mix Span80 and OP-10 evenly to obtain a composite emulsifier, mix the composite emulsifier, mixed powder and deionized water at a mass ratio of 1:10:50, and stir for 1 hour at a stirring speed of 500r / min to obtain a mixed sol , accordi...

example 2

[0023] The liquid medium is composed of 60.0% deionized water, 20.0% peptone, 14.0% beef extract, 4% agar powder, 0.90% sodium bicarbonate, and 1.10% sodium carbonate in terms of mass percentage, and it is sterilized at 121°C for 18 minutes; After Bacillus subtilis and Lactobacillus were inoculated into the liquid medium, they were cultured at a temperature of 35°C, with an inoculum size of 3%, and a stirring speed of 210r / min for 1 day to obtain a bacteria-containing solution. / min, the vacuum degree is 4mbar, and the pumping rate is 28L / min, and it is centrifuged and concentrated for 30min to obtain the bacterium; the starch and xanthan gum are mixed evenly according to the mass ratio of 1:3 to obtain a mixed powder, and the mass ratio is 1:1. Mix Span80 and OP-10 evenly to obtain a composite emulsifier, mix the composite emulsifier, mixed powder and deionized water at a mass ratio of 1:10:50, and stir for 1 hour at a stirring speed of 600r / min to obtain a mixed sol , accord...

example 3

[0025] The liquid medium consists of 60.0% deionized water, 20.0% peptone, 14.0% beef extract, 4% agar powder, 0.90% sodium bicarbonate, and 1.10% sodium carbonate in terms of mass percentage, and is sterilized at 126°C for 20 minutes; After Bacillus subtilis and Lactobacillus were inoculated into the liquid medium, they were cultured at a temperature of 40°C, with an inoculum size of 4%, and a stirring speed of 240r / min for 2 days to obtain a bacteria-containing solution. / min, the vacuum degree is 5mbar, and the pumping rate is 30L / min, and it is centrifuged and concentrated for 40min to obtain the bacterium; the starch and xanthan gum are mixed evenly according to the mass ratio of 1:3 to obtain a mixed powder, and the mass ratio is 1:1. Mix Span80 and OP-10 evenly to obtain a composite emulsifier, mix the composite emulsifier, mixed powder and deionized water at a mass ratio of 1:10:50, and stir for 2 hours at a stirring speed of 650r / min to obtain a mixed sol , according ...

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Abstract

The invention relates to a preparation method of a microbial feed additive, which belongs to the technical field of feed additives. The preparation method comprises the following steps: inoculating aliquid culture medium with bacillus subtilis and lactobacillus, centrifugally concentrating to obtain thallus, preparing thallus sol by virtue of a sol-gel method, i.e. the microbial feed additive. The lactobacillus can improve the non-specific immunity of animals and can generate digestive enzymes such as amylase, lipase and protease and can generate bacteriocin to inhibit the growth of pathogenic bacteria, and the bacillus subtilis is high in resistance, resistant to high temperature, acid, alkali and high pressure, is good in tolerance to the particle feed processing, exists in a spore formin the storage process, does not consume the nutritional ingredients of the feed, can be rapidly revived in intestinal ducts (the revival rate is approximately 100 percent), and can generate variousamino acids and various digestive enzymes such as amylase, protease and lipase to consume oxygen in the intestinal ducts and to maintain the anaerobic environment, thereby inhibiting the growth of thepathogenic bacteria.

Description

technical field [0001] The invention relates to a preparation method of a microbial feed additive, which belongs to the technical field of feed additives. Background technique [0002] In general, microbial feed additives refer to nutritional substances that can have a significant effect on the microecological environment of the gastrointestinal tract of animals, and generally come from symbiotic cultures of animal organisms or their fermented products, usually live bacterial preparations, are It is derived from the digestive tract and acts on the digestive tract, which can effectively supplement the beneficial flora, mediate or maintain the balance of the flora, enhance the inhibition of harmful intestinal bacteria and non-specific immune function, and rapidly improve the body's disease resistance, metabolic level and It is a biologically active additive with multiple functions such as disease prevention, growth promotion and improvement of nutrient digestion and absorption...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163C12N1/20C12R1/125C12R1/225
CPCA23K10/18A23K20/163C12N1/20A23V2400/11
Inventor 胡次兵张鑫骆兵建
Owner FOSHAN SENANG BIO TECH CO LTD
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