Thermally-stable emulsion of antigen as well as preparation method and application of thermally-stable emulsion

Abstract

The invention provides a thermally-stable emulsion of an antigen as well as a preparation method and an application of the thermally-stable emulsion. The thermally-stable emulsion of the antigen comprises a water phase, an oil phase and inorganic particles combined with the antigen; the oil phase is wrapped by the water phase; the inorganic particles combined with the antigen are dispersed on an interface of the water phase and the oil phase; and the antigen and the inorganic particles are combined in an electrostatic adsorption way. By using the emulsion provided by the invention, the thermalstability of the antigen of an organism can be remarkably improved; structural water molecules in a mineral phase are tightly connected to the peripheries of mineralized proteins, so that the hydrogen bond exchange rate of protein molecules and water molecules in an external environment is reduced, the destruction of a thermal motion process to the structures of the proteins is reduced, the stability of a vaccine in a high-temperature environment is improved, the valid period of vaccine storage is prolonged, and the vaccine can be stored at 25 DEG C for three weeks or longer; in addition, theemulsion can also be applied as a vaccine adjuvant, a drug delivery carrier or a controlled-release carrier, a surfactant is prevented from being used, and the poisonousness to human bodies and environment pollution can be reduced.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to an antigen heat-stable emulsion and a preparation method and application thereof. Background technique

[0002] Infectious diseases have long been one of the major threats to human health. As one of the effective means to deal with infectious diseases, vaccination plays an extremely important role in the prevention and control of diseases. However, most of the early vaccines were attenuated or inactivated whole virus vaccines. Although they can stimulate strong protective antibody levels and cellular immune responses, there are also risks of incomplete virulence recovery and inactivation. Therefore, with the development of biotechnology, split vaccines, subunit vaccines, recombinant protein vaccines, synthetic peptide vaccines, and polysaccharide conjugate vaccines with higher safety have gradually replaced attenuated and inactivated vaccines in clinical practice. Especially in recen...

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