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Ocean phosphorus-dissolving bacillus megaterium and application thereof

A technology of Bacillus megaterium and phosphate-solubilizing bacteria, which is applied to marine Bacillus megaterium phosphate-solivating bacteria SCSIO43701 and its application fields, can solve problems such as branch growth and root dry weight, and achieve good growth, accelerated repair, and growth-promoting effects

Active Publication Date: 2019-03-26
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For different plants, plant growth-promoting bacteria can effectively promote plant growth by 500% and increase yield by 57% (Kloepper et al., 1980; Asghar et al., 2004; Khalid et al., 1997), as reported on There have been a lot of reports on terrestrial plants. The shoot growth and root dry weight of the cucumber and pepper experimental groups inoculated with Bacillus megaterium phosphate solubilizers increased by 8%-22% and 27% respectively and up to 26% and 29%. There are not many research reports on the growth promotion of marine Bacillus megaterium phosphate-solubilizing bacteria from deep sea

Method used

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  • Ocean phosphorus-dissolving bacillus megaterium and application thereof
  • Ocean phosphorus-dissolving bacillus megaterium and application thereof
  • Ocean phosphorus-dissolving bacillus megaterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation, purification and identification of Bacillus megaterium SCSIO 43701

[0022]The deep-sea samples used to isolate microorganisms are deep-sea samples from the northern part of the South China Sea (2011), and the specific depth of collection is: 3928m; the site is 118°30.989E, 18°5.255N; the separation medium used is a diluted 10% 221E medium: the specific components are peptone 0.1g, yeast extract 0.5g, FePO 4 0.01g, (NH 4 ) 2 SO 4 0.4g, CaCO 3 0.1g, agar 12g and seawater 1000mL, pH 7.2-7.4. Weigh 0.2g sediment sample into 18mL sterilized seawater, dilute to 10 -1 、10 -2 、10 -3 3 dilutions, spread on the plate, and after obvious colony grows, transfer to spread until a single bacterium is found. The bacterial strain SCSIO 43701 was thus isolated and purified.

[0023] 1.1 Morphological and molecular biological identification of Bacillus megaterium SCSIO 43701

[0024] Through Gram staining and microscopic examination, the results showed...

Embodiment 2

[0036] Example 2: Determination of organophosphate degradation activity of Bacillus megaterium SCSIO 43701

[0037] The method used for the detection of the phosphorus-solubilizing activity of Bacillus megaterium SCSIO 43701 is the molybdenum-antimony antichromogenic method, and a single colony is picked and inoculated into 100 mL of improved selective liquid nitrogen-fixing medium (the specific composition of the improved selective liquid nitrogen-fixing medium is: FeSO 4 ·7H 2 O 0.001g, KCl 0.56g, MgCl 2 ·6H 2 O 4.00g, NaCl 25.00g, MgSO 4 ·7H 2 O4.80g, K 2 HPO 4 0.01g, Tris 0.48g, peptone 4.00g, yeast extract 2.00g, glycerol 2.00mL and deionized water 1.00L, pH 8.10, add 18.00g agar to the solid medium) Erlenmeyer flask, set up the experimental group and the control group , wherein the experimental group was inoculated with 1% bacterium liquid and the control group was inoculated with 1% sterile water, placed at 28°C, and after cultivating at 180rpm for 24h, it was tr...

Embodiment 3

[0038] Example 3: Bacillus megaterium SCSIO 43701 is used for toxicity identification experiment

[0039] This experiment was completed in Guangdong Provincial Microbial Analysis and Testing Center. Pick a single colony of Bacillus megaterium SCSIO 43701 and inoculate it in an improved selective liquid nitrogen-fixing medium (the formulation of the improved selective liquid nitrogen-fixing medium is the same as in Example 2) and cultivate it at 30°C for 3 days, and adjust the concentration of the bacterial solution to 2.0×10 8 ~2.0×10 9 cfu / mL, 20 KM variety SPF grade mice used in the test, half male and half male, the growth environment is room temperature 24-28°C, relative humidity is (40-70)%, raised in group cages, 10 / cage.

[0040] The specific method of the toxicity detection experiment is strictly in accordance with the "General Technical Guidelines for Biosafety of Microbial Fertilizers NY 1109-2006", the dose is 5580mg / kg·BW, and according to the requirements of the...

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Abstract

The invention discloses ocean phosphorus-dissolving bacillus megaterium and an application thereof. The ocean phosphorus-dissolving bacillus megaterium is collected in China Center for Type Culture Collection (CCTCC), the address is Wuhan University, Luojia Hill, Wuhan, China, and the collection number is CCTCC NO:M2018056. The ocean phosphorus-dissolving bacillus megaterium and the application disclosed by the invention have the beneficial effects that phosphates can be degraded, the phosphorus-dissolving bacillus is about 117.14mg / dl when sodium phytate is used as a unique sphosphorus source, and by high-efficiency degradation of substances such as phosphates in a deposit environment, high-efficiency circulation of phosphorus elements in an ecological system is promoted, and the obtainedphosphorus amount in the deposit environment is increased; the growth of mangrove-plant kandelia candel can be better promoted, the productivity of the whole ecological system can be improved and therestoration of the ecological system can be accelerated; due to no toxic substances, the ocean phosphorus-dissolving bacillus megaterium can be further applied to research and development of microbial agents with microbial functions, has large potential for assisting protection and restoration of the mangrove ecological system, and has better market prospect, economic benefit and ecological effect.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a marine Bacillus megaterium SCSIO 43701 and its application. Background technique: [0002] Plant Growth Promoting Bacterium (PGPB) refers to a class of bacteria that can effectively promote plant growth and yield by increasing nutrient availability, producing hormones, and inhibiting plant harmful microorganisms; the most common PGPR species: Agrobacterium Agrobacterium, Arthrobacter, Azotobacter, Azospirillum, Bacillus, Burkholderia, Caulobacter ), Chromobacterium, Erwinia, Flavobacterium, Micrococcus, Pseudomonas and Serratia (Bhattacharyya and Jha, 2012; RufusJ.Akinrinlola, 2018), on the one hand, plant growth-promoting bacteria directly promote the growth of plants by directly providing nutrients and plant hormones, and can promote the growth of plants by inhibiting the effect of plant pathogens (Ahemad and Kibret, 2014); and can effectively improve the substrate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P21/00C12R1/11
CPCA01N63/00C05F11/08C12N1/205C12R2001/11
Inventor 凌娟董俊德张燕英王聪林丽云杨清松张颖
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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