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Preparation method and applications of black fungus composite small molecule peptide powder

A technology of small molecular peptides and black fungus, applied in the field of food industry, can solve the problems of high diet cost and insufficient effect of auxiliary treatment

Inactive Publication Date: 2019-03-29
大连木耳肽国际贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these eating methods, when black fungus is eaten as a dish, the human body’s absorption rate of the active ingredient polypeptide is only 5-8%, resulting in extremely high dietary costs for daily adjuvant treatment of related diseases, and the effect of adjuvant treatment is not obvious enough

Method used

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  • Preparation method and applications of black fungus composite small molecule peptide powder
  • Preparation method and applications of black fungus composite small molecule peptide powder
  • Preparation method and applications of black fungus composite small molecule peptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Example 1: Preparation of Black Fungus Polypeptide Dry Powder

[0122] 1. Take 100 kg of black fungus, select, wash, and crush in a mechanical roller. Add 5 times the weight of black fungus in distilled water at 55°C, and crush the black fungus tissue and homogenate for 2 hours in a high-pressure homogenizer.

[0123] 2. Use 6.0mol / L HCl solution to control the pH value to 5.5. According to the mass concentration of the substrate is 5%, according to the amount of Aspergillus oryzae exocutase 8%, the amount of papain solution 15%, under the constant temperature condition of 55 ℃, the amount of Aspergillus oryzae flavored enzyme is 5%, successively use exonuclease to act for 20 hours , papain acts for 60 hours, Aspergillus oryzae flavor enzyme acts for 10 hours.

[0124] 3. Cool to room temperature, centrifuge at 3000r / min for 5 minutes, inactivate the supernatant at 70°C for 50 minutes, and concentrate under reduced pressure to obtain a concentrated peptide solution; a...

Embodiment 2

[0127] Example 2. Detection of black fungus polypeptides

[0128] 1 Materials and methods

[0129] 1.1 Raw materials and instruments The black fungus protein isolate homogenate in Example 1, protein content ≥ 89%; Neutral protease, Wuxi Genenco Bioengineering Co., Ltd., Xuemei brand (food grade); Alkaline protease, Wuxi Genen Ke Biological Engineering Co., Ltd., Xuemei brand (food grade); peptide molecular weight standard, Amersham Bio2sciences company, dedicated to capillary electrophoresis; microwave oven, LG Electronics Co., Ltd., MS22089T; formaldehyde, Xi’an Chemical Reagent Factory, analytically pure; high-efficiency capillary electrophoresis instrument , CE2212, manufactured by Peking University; gel permeation chromatography, 24142515, Waters, USA.

[0130] Detection method

[0131] 1. Formaldehyde titration method for detection of degree of hydrolysis and amino nitrogen (GB / T 14771-1993). The degree of hydrolysis is defined as the percentage of the peptide bond in ...

Embodiment 3

[0145] Example 3. Black fungus polypeptide is used to prepare granules

[0146] The concentrate obtained in step 3 was thoroughly mixed and spray-dried. 1. The black fungus small molecular active peptide dry powder obtained in Embodiment 1 is made into granules of certain specifications with a granulator, and is packed into required various quantitative packages with a vacuum packaging machine to make granules.

[0147] 5. Packaging of granules: 5g / bag, 20 bags / box.

[0148] Dosage and usage: Once in the morning and evening, take 1 bag each time, take it with warm water.

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Abstract

The invention discloses a preparation method and applications of a black fungus composite small molecule peptide powder. The prepared black fungus polypeptide is easy to absorb. The test results showthat multiple symptoms of sub-health can be relieved. The powder can be used to prepare multiple kinds of functional food and has a good market prospect.

Description

technical field [0001] The invention belongs to the field of food industry and relates to a plant peptide component extraction technology, in particular to a preparation method and application of black fungus polypeptide. technical background [0002] Black fungus contains colloidal protein and water-soluble fiber, which have obvious effects on bowel cleansing, blood vessel clearing, and lung clearing. Black fungus colloidal protein and water-soluble fiber can clean up intestinal waste, increase intestinal peristalsis, and treat constipation. Lung waste has a significant effect. It is a health care product for dust pollution industries such as hairdressing and textile workers. Black fungus contains protein, fat, crude fiber, calcium, potassium, iron, zinc, selenium, vitamin E, vitamin D, thiamine, riboflavin, niacin, folic acid, fungal polysaccharides and other ingredients. The crude fiber contained in it can promote gastrointestinal motility, relieve constipation, remove ...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J3/34A23J3/00A23L33/18
CPCA23J1/00A23J3/00A23J3/34A23V2002/00A23L33/18A23V2200/30A23V2200/3324A23V2200/3262A23V2200/324A23V2200/326A23V2200/328A23V2200/302A23V2200/304A23V2200/334A23V2250/55
Inventor 许忠海
Owner 大连木耳肽国际贸易有限公司
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