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Strain of bacillus lacticus DU-106 with probiotic properties and application thereof

A technology of Bacillus lactobacillus, DU-106, applied in the field of bioengineering, can solve the problems of poor preservation, low heat resistance, and small number of viable bacteria, and achieve strong resistance to stress, enhance immunity, and improve intestinal function. Effect

Inactive Publication Date: 2019-06-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects of existing lactic acid bacteria such as low heat resistance, poor preservation, and a small number of viable bacteria entering the body, and provide a lactic acid strain with strong ability to produce lactic acid, resist drying, resist high temperature, resist gastric acid and resist bile. Bacillus DU-106;

Method used

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  • Strain of bacillus lacticus DU-106 with probiotic properties and application thereof
  • Strain of bacillus lacticus DU-106 with probiotic properties and application thereof
  • Strain of bacillus lacticus DU-106 with probiotic properties and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Sample Collection, Strain Isolation and Screening

[0016] 1.1 Isolation and screening medium

[0017] Lactic acid bacteria (MRS) liquid culture medium: protein building 1%, beef extract 1%, yeast powder 0.5%, glucose 2%, dipotassium hydrogen phosphate 2.0%, diammonium hydrogen citrate 0.2%, sodium acetate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, cysteine ​​0.05%, calcium carbonate 0.05%, Tween 80 1.0ml, adjust the pH to 5.4-6.40, and sterilize at 115°C for 20min.

[0018] Lactic acid bacteria proliferation medium (YPG medium) (g / L): 5.0g glucose, 5.0g protein, 1.0g yeast extract, 0.1g calcium chloride, 15g agar, 1000mL distilled water, adjust pH to 6.5, extinguish at 115°C Bacteria 20min.

[0019] Solid medium: add 1.5% agar on the basis of liquid medium.

[0020] 1.2 Sample Collection

[0021] The isolated samples were collected in 2001 from Inner Mongolia herdsmen's traditional naturally fermented yoghurt.

[0022] 1.3 Isolation and scr...

Embodiment 2

[0024] The identification of embodiment 2 screening bacterial strains

[0025] 2.1 Morphological identification

[0026] figure 2 It is the form of the DU-106 strain after static culture for 24 hours. The DU-106 strain is a facultative anaerobic bacteria and grows well in static culture.

[0027] The colony morphology of DU-106 is as follows image 3 Show: colony diameter 2.0-2.5mm, calcium dissolution circle phenomenon is obvious, platform shape, edge is neat, opaque, shiny, without pigment, texture is soft. On the MRS liquid medium, it is uniformly turbid with a small amount of precipitation, and the precipitation will be scattered when it is gently shaken, without forming a bacterial film and a bacterial ring. At the initial stage of culture, the cell morphology was observed under the microscope as rod-shaped, terminal spores, cysts were not enlarged, and Gram staining was positive. The growth temperature range of the strain is 10-45° C., and the growth pH value range ...

Embodiment 3

[0043] Embodiment 3 acute toxicity test

[0044] The acute toxicity test was carried out according to the maximum tolerated dose method in GB 15193.3-2014. The test results showed that no poisoning and death occurred in all mice. The experimental results showed that: Lactobacillus DU-106 strain sample mice oral LD 50 >10.0g / kg.b.w, it is a non-toxic substance according to the classification standard of acute toxicity.

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Abstract

The invention discloses a strain of bacillus lacticus DU-106 with probiotic properties and application thereof. The bacillus lacticus is preserved in the Guangdong Provincial Microbial Culture Collection on March 22, 2019, and the preservation number is GDMCC 60621. The bacillus lacticus can tolerate the environment in the digestive tract, is resistant to gastric acid and bile salt, has strong stress resistance, can be planted in the intestinal tract in a large amount in the form of living bacteria, is resistant to high temperature and dryness, and can be well added into food for processing and application. The bacillus lacticus also has the probiotic application such as improving the intestinal function of animals, assisting in lipid-lowering, enhancing the immunity and assisting in bloodsugar lowering.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a lactic acid bacillus with probiotic properties and an application thereof. Background technique [0002] Lactic acid bacteria is a general term for a group of bacteria that can use fermentable carbohydrates to produce large amounts of lactic acid, and it is also a probiotic that exists in humans. Lactic acid bacteria can improve the body's digestion and absorption of protein, lactose and calcium and other nutrients, can produce a variety of vitamins needed by the body, can also inhibit the reproduction of spoilage bacteria and pathogenic bacteria in the intestinal tract, and reduce blood ammonia and blood cholesterol. It can also improve the immune activation of the body and maintain the balance of intestinal flora. Therefore, lactic acid bacteria are widely used in many fields such as fermented food, beverage, feed and medicine. Current research and applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/744A61P1/00A61P3/06A61P37/04A61P3/10A61P1/14C12R1/07
Inventor 杜冰黄剑钊黎攀蒋卓肖南曾丽萍田文妮温嘉敏赖玉健
Owner SOUTH CHINA AGRI UNIV