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Ferulic acid degrading micro-organism strain in coptis chinensis soil and use thereof

A technology of ferulic acid and degrading bacteria, applied in the field of microorganisms, can solve problems such as restricting the development of Coptis chinensis industry, and achieve the effects of easy preparation, convenient storage conditions and simple fermentation process

Active Publication Date: 2019-09-17
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, after planting a round of Coptis chinensis, the soil needs to rest for 5-10 years before it can be planted again, which severely limits the development of Coptis chinensis industry.

Method used

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  • Ferulic acid degrading micro-organism strain in coptis chinensis soil and use thereof
  • Ferulic acid degrading micro-organism strain in coptis chinensis soil and use thereof
  • Ferulic acid degrading micro-organism strain in coptis chinensis soil and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Materials and methods

[0032] 1.1 Sample collection and processing

[0033] The sample was rhizosphere soil of five-year-old Coptis chinensis, which was collected from the Coptis chinensis planting base in Shizhu County, Chongqing (108°23′E, 30°15′N) in October 2016, and brought back to the laboratory in a sterile bag for processing on the same day.

[0034] 1.2 Separation and purification of microorganisms in soil

[0035] After removing impurities such as roots, fallen leaves, and stones in the soil sample, pass through a 20-mesh sieve, add 5 grams of soil sample to 50 ml of sterile water, dilute 10,000 times, and evenly coat the plate before adding chloramphenicol (25 mg / ml) on potato dextrose (PDA) solid medium. Cultivate at 25°C for 3 to 5 days, and after the formation of visible colonies, transfer to a solid medium without anti-PDA for purification.

[0036] 1.3 Phenolic Acid Bacteria Reduction Screening

[0037] Pick a single colony from the isolated and ...

Embodiment 2

[0070] Embodiment 2 ferulic acid degrading bacteria single component preparation

[0071] F-0056 spore powder preparation method:

[0072] Streak the preserved F-0056 strain on the PDA solid medium and culture it at 25°C for 2 days to activate it. Pick a single colony of the activated fungus on the PDA solid medium, culture at 25° C. for 3 to 4 days, and wash the spores on the medium with sterile water. The spores were washed twice with sterile water and freeze-dried to make spore powder.

[0073] The effective number of bacteria can be 1×10 9 Cfu / g of F-0056 spore powder, dissolved in an amount of 100g / mu and then poured into the soil after one round of stubble planting, can quickly degrade the phenolic acids accumulated in the soil and achieve the purpose of repairing the soil;

[0074] The preparation can be used as a single preparation for soil ferulic acid degradation, and can also be mixed into other soil preparations as a soil repair additive.

Embodiment 3

[0075] Embodiment 3 ferulic acid degrading bacteria combination preparation

[0076] The ferulic acid-degrading bacteria single component preparation prepared in Example 2 was mixed with Trichoderma harzianum, Bacillus subtilis and other biocontrol bacteria and organic fertilizers to make a mixed preparation, and the dosage form was powder. In the mixed preparation, the effective number of viable bacteria of Coptidis soil ferulic acid degrading bacteria F-0056 was 1×10 9 cfu / g.

[0077] The composition of the combined preparation comprises: 80-85% of straw powder, 10-15% of compound fertilizer, 1% of binder and 2% of formula bacteria.

[0078] Before the transplanting of Coptidis seedlings, the combined preparation containing F-0056 effective bacteria was used to stir the soil at an application rate of 100 g / mu, which could effectively inhibit the fungus caused by Phytophthora solani, Rust rot and Black spot during the growth of Coptis chinensis. Root diseases, slow down the...

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Abstract

The present invention discloses a ferulic acid degrading micro-organism strain in coptis chinensis soil and a use thereof, and belongs to the field of microorganisms. A strain of penicillium daleae has a high-efficiency degradation effect on soil allelochemical of ferulic acid, also has a micro-organism-inhibiting effect against plant pathogens of rhizoctonia solani, cylindrocarpon destructans, alternaria panax, etc., and has repairing and biocontrol functions for continuous cropping soil. The strain is isolated and purified from the soil of coptis chinensis at five years old in Shizhu county. After a ferulic acid degrading effect of the strain is determined, species is identified by ITS, ferulic acid degrading efficiency of the strain under different ferulic acid addition amount and different fermentation time is tested, and finally, the best fermentation time is determined to be 96 hours, and the degrading efficiency is significantly reduced when the addition amount of the ferulic acid in a culture medium is more than 800 [mu]g / ml. The strain is preserved in China General Microbiological Culture Collection Center on May 9, 2018 and has a preservation number of CGMCC No.15675.

Description

technical field [0001] The invention relates to a Coptis chinensis soil ferulic acid degrading bacterium and application thereof, belonging to the field of microorganisms. Background technique [0002] Coptis chinensis is a perennial herb of Ranunculaceae, and its rhizome is in the shape of yellow beads. As a traditional Chinese medicine, it is often used to treat gastrointestinal diseases such as dampness and heat in the stomach and vomiting. The active ingredient is berberine (also known as berberine). Berberine is widely used in Chinese and Western medicine clinically. It has the effect of lowering total cholesterol, triglyceride and low-density lipoprotein cholesterol in serum, and has the effect of lowering blood sugar and protecting cardiovascular system. [0003] Rhizome medicinal materials, including Coptis chinensis, Pseudostellariae, and Rehmannia glutinosa, generally have continuous cropping obstacles caused by changes in soil physical and chemical properties an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01B79/02C12R1/80
CPCA01B79/02C12N1/145C12R2001/80
Inventor 胡昌华廖国建刘云露郑守豪秦瑜
Owner SOUTHWEST UNIV
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