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Microelectrodes and probes containing microelectrodes and their use for determining oxygen content in the brain

A technology of oxygen content and microelectrode, which is applied in the direction of measuring devices, electrochemical variables of materials, diagnostic recording/measurement, etc., can solve problems such as brain tissue damage, hydrogen peroxide interference, poor electrode reproducibility, etc., to improve stability, The effect of reducing complexity and broad application prospects

Active Publication Date: 2021-01-26
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention aims to solve the technical problems of hydrogen peroxide interference, poor electrode reproducibility, and brain tissue damage caused by products in the analysis process existing in the existing living body in situ oxygen detection technology

Method used

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  • Microelectrodes and probes containing microelectrodes and their use for determining oxygen content in the brain
  • Microelectrodes and probes containing microelectrodes and their use for determining oxygen content in the brain
  • Microelectrodes and probes containing microelectrodes and their use for determining oxygen content in the brain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of single-atom catalyst material ISAs-Co / HNCS:

[0036] First, the silica pellets of the material substrate were prepared by the classical co-precipitation method, which was completed by the condensation of TEOS in water and ethanol solution and the catalytic hydrolysis of ammonia. The specific steps are as follows: TEOS is quickly injected into water, ethanol and ammonia water, the solution is stirred for 10 hours, and the precipitate is obtained by centrifugal suction filtration. The precipitate was rinsed with ethanol three times and then left to dry, and then silica pellets with a size of about 190 nm in diameter were obtained, which were used as material synthesis substrates.

[0037] Then Co-TIPP, TIPP and the above-mentioned silica pellets were placed together in a round-bottomed flask, supplemented with DMF as a solvent, and finally injected with α,α-dibromo-p-xylene, and stirred under heating at 110°C for one day. After centrifugation, the product ...

Embodiment 2

[0047] 1. Use the single-atom carbon fiber electrode obtained by the method of Example 1 to detect oxygen in situ in vivo

[0048] Before using the carbon fiber electrode modified ISAs-Co / HNCS in step S200 for in situ detection in vivo, it needs to be confirmed in vitro that the electrode obtained by the above method has good selectivity to oxygen. The inventors compared and detected the responses of common electrochemical interfering substances in the brain on electrodes. refer to Figure 2A and Figure 2B , apply a voltage of -0.1V to the working electrode, and after the background current is stable, add electrolyte solution (artificial cerebrospinal fluid is used here, its composition is NaCl (126mmol / L), KCl (2.4mmol / L), KH 2 PO4(0.5mmol / L), MgCl 2 (0.85mmol / L), NaHCO 3 (27.5mmol / L), Na 2 SO 4 (0.5mmol / L), CaCl 2 (1.1mmol / L), used to simulate the cerebrospinal fluid environment), add 20μmol / L dopamine (DA) solution, 20μmol / L epinephrine (E) solution, 20μmol / L norepi...

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Abstract

The present invention proposes a microelectrode and a probe containing the microelectrode and its application in measuring oxygen content in the brain, the microelectrode comprises: a carbon fiber electrode; a hydrophobic modification layer, the hydrophobic modification layer is formed on the carbon fiber electrode an outer surface; and a catalyst modification layer, the catalyst modification layer is formed on the outer surface of the hydrophobic modification layer, and the catalyst modification layer is formed by ISAs-Co / HNCS. The microelectrode of the present invention can effectively detect the oxygen content, especially the in-situ detection of oxygen in the brain, realizes the direct four-electron oxygen reduction process, and at the same time is not disturbed by the hydrogen peroxide reduction current, avoiding the generation of excessive cytotoxic hydrogen peroxide, With a controllable and stable electrode interface, the electrode detection has strong stability and good repeatability, and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of materials and electrochemical analysis. In particular, the present invention relates to microelectrodes and probes containing microelectrodes and their use in the determination of oxygen levels in the brain. Background technique [0002] Brain neurochemistry has gradually become a hot research field. Through the detection of neuroactive substances in the brain, electrochemical methods can reveal the molecular mechanism and provide the most direct information for neuroscience research. Oxygen, as an important neurochemical small molecule in the brain, is related to many physiological and pathological processes in the human body. Therefore, the accurate measurement of oxygen concentration is of great significance to the study of physiological and pathological processes in vivo. Among the various existing oxygen detection methods, electrochemical methods have attracted wide attention due to their advantage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48G01N31/10A61B5/1477A61B5/1473
CPCA61B5/1473A61B5/1477G01N27/308G01N27/48G01N31/10
Inventor 毛兰群潘聪薛亦飞于萍
Owner INST OF CHEM CHINESE ACAD OF SCI
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