Application of wx mutant protein and its gene in plant breeding based on gene editing technology

Abstract

The invention discloses a rice Wx mutant protein, a mutant gene and applications thereof. The invention also discloses a breeding method for using gene editing to create quality-improved rice with transparent appearance and appropriately reduced amylose content. The present invention uses gene editing technology for the first time to wx Gene undergoes single base editing, through progeny screening, in T 2 A new material with T-DNA removed, transparent appearance, appropriately reduced amylose content, improved quality, and stable inheritance can be obtained. Compared with breeding such as chemical mutagenesis and hybridization, gene editing-directed molecular breeding technology has the advantages of rapidity, precision, and efficiency. It can be combined with gene function marker genotype selection, or directly use other mature rice varieties as background materials for transformation and acquisition. The corresponding materials will greatly improve the breeding efficiency and greatly speed up the breeding process.

Description

technical field [0001] The invention belongs to the fields of crop genetic breeding, new resource innovation for crop quality improvement, and excellent edible crop breeding, and particularly relates to the application of a Wx mutant protein and its gene based on gene editing technology in plant breeding. Background technique [0002] Rice (Oryza sativa L.) is an important food crop in China and even in the world, and plays a pivotal role in ensuring food security. [0003] Rice quality refers to the basic characteristics that rice must have in commodity circulation, including four main aspects: grinding quality, appearance quality, cooking and eating quality, and nutritional quality, among which appearance quality and cooking and eating quality are particularly important. Seed endosperm is the main edible part of rice. From the chemical composition analysis, the main component of endosperm is starch, which generally accounts for 90% of the dry weight of the endosperm, or ev...

AI Technical Summary

Problems solved by technology

For quality traits, backcrossing is a commonly used method. Generally, 4-6 generations of backcrossing are required, and at least 3-5 generations are required for homozygous selfing. To improve a single trait and play a role in production, at least 6-8 years, the breeding cycle is long, it is difficult to meet the urgent needs of the market and the people for high-quality soft fragrant rice varieties

If we want to tap new soft rice genetic resources, we mainly rely on chemical mutagenesis at present. As we all know, the frequency of chemical mutations is low, and the initial investment is large. It may take 2-3 years to screen and obtain a stable material. At the same time, chemical mutagenesis may cause wild There are multiple gene mutations in the type material, and it needs to be improved through hybridization to eliminate bad gene mutations when it is applied to production

[0007] 2. Most soft rice japonica rice varieties have poor appearance quality

For single-base mutations, enzyme-cut target markers can be designed, but the process is relatively cumbersome. Allele-specific PCR is developed, and genotypes with high and low amylose content can be distinguished by two PCRs

[0013] At present, if scientific and technical personnel want to obtain new materials or new genes of soft rice with AC reduced to 8-12% and the appearance remains transparent, they need to undergo chemical or radiation mutagenesis, which requires a lot of work and the effect is not ideal.

Simultaneously, adopt conventional chemical mutagenesis and conventional transgenic breeding, its breeding life will be at least 6-8 years, and the breeding cycle is long, be difficult to satisfy the urgent demand of market and common people to high-quality soft fragrant rice variety

[0014] At the same time, there is no relevant report on the use of gene editing technology to perform site-directed mutations on the Wx gene of rice varieties to create new soft rice alleles

Images