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A kind of nano fluorescent probe and its preparation method for rapid detection of hydrogen sulfide

A nano-fluorescent probe, fluorescent probe technology, applied in chemical instruments and methods, fluorescence/phosphorescence, color-changing fluorescent materials, etc., can solve the problem of not yet retrieved fluorescent nano-probes, and achieve high sensitivity, broad application prospects, good selective effect

Active Publication Date: 2022-03-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, few documented examples of electrochromic materials being applied to biosensing and imaging of living systems
[0006] Up-conversion and EM1 / PAA-based fluorescent nanoprobes and their H 2 Reports of domestic and foreign literature and patents on S detection

Method used

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  • A kind of nano fluorescent probe and its preparation method for rapid detection of hydrogen sulfide
  • A kind of nano fluorescent probe and its preparation method for rapid detection of hydrogen sulfide
  • A kind of nano fluorescent probe and its preparation method for rapid detection of hydrogen sulfide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of fluorescent probes

[0046] 1. The preparation steps are as follows:

[0047] (1) Synthesis of metal chlorides: add thulium oxide (Tm 2 o 3 , 168 mg), distilled water (3 mL) and hydrochloric acid (3 mL) and heated until the powder was completely dissolved. After refluxing for 30 minutes, the mixture was cooled to room temperature. The solution was then transferred to a 10 mL volumetric flask and made up to 10 mL with distilled water. Thulium chloride solution was used in the next step.

[0048] Similarly, solutions of yttrium chloride, erbium chloride, and ytterbium chloride were prepared according to the protocol for thulium chloride.

[0049] (2)NaYF 4 : Yb, Tm @NaYF 4 : Synthesis of Yb, Er: synthesized by thermal decomposition; RECl containing Y, Yb and Tm 3 (1 mmol / L) (ratio=75%:25%:0.3%) was added to the four-necked flask, and then 1-octadecene (15 mL) and oleic acid (6 mL) were added to the above mixture. After nitrogen evacuation at room t...

Embodiment 2

[0057] Probe PAAO-UCNPs-EM1 and H 2 High resolution mass spectrum of the solution after S response

[0058] The preparation steps of the PAAO-UCNPs-EM1 probe solution and NaHS solution involved in this example are the same as in Example 1, and other specific steps are as follows: Add NaHS solution (100 μmol / L, 10 μL) to the probe solution prepared in Example 1 After reacting for 30 seconds, the reacted solution was diluted with distilled water, and its mass spectrum peak was measured with an ABI Mariner ESI-TOF spectrometer.

[0059] as attached figure 2 As shown, the peak (684.41224) of EM2 appears in the solution after the reaction, and this result shows that the probe solution and H 2 The mechanism of S response is that EM1 connected to the probe interacts with it and generates EM2.

Embodiment 3

[0061] The preparation steps of the PAAO-UCNPs-EM1 probe solution and NaHS solution involved in this embodiment are the same as in Example 1, and other specific steps are as follows: NaHS solutions (0-10 μmol / L) After reacting for 30 seconds, the reacted solution was irradiated with a 980nm laser (100mw) externally connected to the F97pro fluorescence spectrophotometer, and its fluorescence emission spectrum was obtained.

[0062] as attached image 3 As shown, we can see that with H 2 With the increase of S concentration, the fluorescence emission intensity of the reacted probe solution at 540nm and 660nm is increasing, and it is known from the calculation that the probe PAAO-UCNPs-EM1 has an effect on H 2 The detection limit of S is low, and the detection range can reach 0.01-0.13 μmol / L, which shows that the detection effect of the probe is good and the sensitivity is high.

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Abstract

The invention discloses a nanometer fluorescent probe for rapid detection of hydrogen sulfide and a preparation method thereof. The fluorescent probe is PAAO-UCNPs-EM1, and the preparation method is as follows: firstly, UCNPs are prepared by a thermal decomposition method, and then ultrasonically assisted in A stock solution of the probe PAAO‑UCNPs‑EM1 was prepared by self-assembly in solution; due to the fluorescence resonance energy transfer (FRET) effect, the upconversion fluorescence of PAAO‑UCNPs‑EM1 was quenched and hydrogen sulfide (H 2 S) Converting EM1 to EM2 inhibits the FRET effect and causes the fluorescence recovery of PAAO‑UCNPs‑EM2; the peak intensities of the upconversion luminescence of the probe solution at 540 nm, 660 nm and 800 nm are consistent with those of H 2 There is a linear relationship between the S concentration and the H 2 S detection has a low detection limit and can be used for H in biomedical samples 2 Highly sensitive and selective detection of S.

Description

technical field [0001] The invention relates to a fluorescent probe for recognizing hydrogen sulfide and a preparation method thereof, belonging to the field of small molecule fluorescent probes. Background technique [0002] Hydrogen sulfide (H 2 S) is the third most important gas signaling molecule involved in living systems after nitric oxide and carbon monoxide. It plays a multifunctional role in regulating a wide range of physiological processes, such as neurotransmission, vasodilation, renal excretion, inflammation, cytoprotection from oxidative stress, and ischemia-reperfusion injury. In mammalian cells, endogenous H 2 The production of S usually depends on the catalyzed synthesis of cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS). h 2 S can also be produced from D-cysteine ​​and is catalyzed by D-amino acid oxidase (DAO) and 3-thioglycolate sulfurtransferase (3-MST). H in human tissue 2 The concentration of S can be in the micromolar range. Used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/85C09K9/02G01N21/64G01N21/78
CPCC09K11/025C09K11/02C09K11/7773C09K9/02G01N21/6428G01N21/6458G01N21/78C09K2211/1007
Inventor 王鹏崔梦园任翔宇刘天广徐艳琪
Owner CHINA PHARM UNIV
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