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Preparation method of hippophae rhamnoides and probiotic microcapsule and hippophae rhamnoides and probiotic microcapsule product prepared by preparation method

A technology of probiotics and microcapsules, which is applied in the field of seabuckthorn probiotic microcapsule products, can solve the problems of ineffective use of active ingredients, short probiotic activity retention time, and difficulty in ensuring probiotics, etc., to achieve the best proliferation effect , good enteric solubility, high content of live bacteria

Pending Publication Date: 2021-03-26
HENAN BUSINESS SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the transportation, sales and consumption of liquid food, the number of live bacteria contained in probiotics has been decreasing, and the stress resistance of probiotics is poor. When passing through the stomach and reaching the intestinal tract, they are subjected to strong gastric acid, bile, gastric protease and other substances, so it is difficult to ensure that the probiotics reach the intestinal colonization and have a sufficient number of viable bacteria to exert their probiotic effects
[0004] Aiming at the problem that the active ingredients in seabuckthorn are easily destroyed during processing, which leads to the problem that the active ingredients cannot be effectively used and the technical problem that the activity of probiotics is kept for a short time, a kind of enteric-coated probiotic with good storage stability and good gastric acid resistance is developed. The preparation method of probiotic preparation with excellent effect is an urgent problem to be studied and solved in this technical field

Method used

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  • Preparation method of hippophae rhamnoides and probiotic microcapsule and hippophae rhamnoides and probiotic microcapsule product prepared by preparation method
  • Preparation method of hippophae rhamnoides and probiotic microcapsule and hippophae rhamnoides and probiotic microcapsule product prepared by preparation method
  • Preparation method of hippophae rhamnoides and probiotic microcapsule and hippophae rhamnoides and probiotic microcapsule product prepared by preparation method

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Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Activation and proliferation of probiotic strains

[0037] In order to study the influence of different culture media on the activation of probiotics, the present invention uses different culture media to activate the probiotics, the details of which are as follows.

Embodiment 1-1

[0038] Embodiment 1-1: Selection of activation medium

[0039]Prepare 25 mL of glucose basal medium, galactose proliferation medium, and sucrose proliferation medium respectively; the carbon source concentration in each medium is set to six concentration gradients of 0.125%, 0.25%, 0.5%, 1.0%, 2.0%, and 4.0% After the preparation of the culture medium, it was sterilized at 121°C for 20 minutes.

[0040] The composition of the glucose basal medium is: peptone 10.0g / L, beef extract powder 5.0g / L, yeast extract powder 4.0g / L, glucose 20.0g / L, Tween-80 1.0ml / L, dipotassium hydrogen phosphate 2.0g / L, sodium acetate 5.0g / L, triammonium citrate 2.0g / L, magnesium sulfate 0.05g / L, the balance is water; the pH of the glucose basal medium is 6.2-6.4.

[0041] The composition of galactose proliferation medium is: peptone 10.0g / L, beef extract powder 5.0g / L, yeast extract powder 4.0g / L, galactose 20.0g / L, Tween-80 1.0mL / L, hydrogen phosphate Dipotassium 2.0g / L, sodium acetate 5.0g / L, tri...

Embodiment 1-2

[0045] Embodiment 1-2: Effect of galactose proliferation medium on the growth process of probiotics

[0046] Prepare 25mL glucose basal medium (the concentration of glucose in the glucose medium is 2%) and galactose proliferation medium (the concentration of galactose in the galactose proliferation medium is 2%). Sterilize for 20 minutes. The preserved Lactobacillus bulgaricus and Bifidobacterium bifidum were inserted into each of the sterilized medium, and cultured anaerobically at 37° C. for 48 hours. The absorbance of the sterile solution was measured at 600 nm every 4 h. The absorbance measurement results were as figure 2 shown.

[0047] Depend on figure 2 It can be seen that the probiotics enter the logarithmic growth phase after being cultured in the galactose proliferation medium for 8 hours, and enter the logarithmic growth phase after being cultivated in the glucose-based medium for 12 hours. OD600 nm value was significantly higher than that of glucose basal me...

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Abstract

The invention relates to a microcapsule, and particularly discloses a preparation method of a hippophae rhamnoides and probiotic microcapsule. The method comprises the following steps of 1) inoculating probiotics into a hippophae rhamnoides extracting solution, and carrying out fermentation culture for 1-3 days to obtain a hippophae rhamnoides and probiotic fermentation solution; 2) dissolving sodium alginate in water to prepare a sodium alginate solution with the concentration of 1%-10%, and dissolving whey protein isolate with an acid solution to prepare a whey protein isolate solution withthe concentration of 3%-12%; 3) uniformly mixing the hippophae rhamnoides and probiotic fermentation solution, the sodium alginate solution and the whey protein isolate solution in proportion to obtain a mixed solution; and (4) dropwise adding the mixed solution obtained in the step (3) into a calcium chloride solution, performing fixing for 20-40 minutes at room temperature, performing filtering,and removing a water phase to obtain the hippophae rhamnoides and probiotic microcapsule. The hippophae rhamnoides and probiotic microcapsule prepared by the method is high in nutritional activity, high in probiotic viable count and strong in environmental stress resistance.

Description

technical field [0001] The invention relates to a microcapsule, in particular to a preparation method of a seabuckthorn probiotic microcapsule and a prepared seabuckthorn probiotic microcapsule product. Background technique [0002] Seabuckthorn (scientific name: Hippophae rhamnoides Linn.) is a plant resource with both medicinal and edible uses. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and bioactive substances, rich in vitamins C. Flavonoids, polysaccharides, amino acids, unsaturated fatty acids and trace elements, etc. Among them, the content of VC is very rich, known as the king of vitamin C. Seabuckthorn fruit used as medicine has the effects of relieving cough and reducing phlegm, invigorating stomach and eliminating food, promoting blood circulation and dispelling blood stasis. Modern pharmacological research has also found that the active ingredient flavonoids has the effects of anti-f...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/16A23L33/19A23L29/256B01J13/02
CPCA23L33/135A23L29/256A23L33/19A23L33/16B01J13/02A23V2002/00A23V2200/3204A23V2250/5026A23V2250/54252A23V2250/1578A23V2200/324
Inventor 周莉王法云章建军朱海华谭静平洋张亚勋王晓瑞罗蓓蓓王鋆坦王永王慧
Owner HENAN BUSINESS SCI RES INST
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