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Composite biological decomposition agent and preparation method thereof

A decomposing agent and compound enzyme preparation technology, which is applied in the field of organic waste treatment and resource utilization, can solve the problems of inability to crops, absorption, and inability of nutrients to be effectively degraded. The effect of shortening the decay time

Active Publication Date: 2021-04-16
辽宁振兴生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while many enterprises are responding to national policies, the organic materials used have not been thoroughly and effectively decomposed. The materials that have not been thoroughly decomposed also contain many unfavorable factors, including pathogenic bacteria, weed seeds, insect eggs, antibiotics, and heavy metals. , toxic and harmful gases, etc., and the nutrients cannot be effectively degraded and cannot be absorbed by crops. Although the organic fertilizer produced by such materials can improve the physical structure of the soil, the negative factors brought about are conceivable

Method used

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  • Composite biological decomposition agent and preparation method thereof
  • Composite biological decomposition agent and preparation method thereof
  • Composite biological decomposition agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation one of composite biological decomposing agent:

[0030] a. Preparation of thermophilic Clostridium papyrus: (1) Seed culture: inoculate the colony on the plate culture medium into the seed culture solution, and cultivate it in a shaking table at 55~60°C for 16~24h, with a rotating speed of 200~260rpm;

[0031] Among them, the seed culture solution is: 50g cellobiose solution, 1g tryptone, 0.5g yeast extract, 0.15g ammonium chloride, 0.5g urea, 0.2g tripotassium citrate, 0.15g citric acid monohydrate, 0.1g Potassium dihydrogen phosphate, 0.1g of sodium chloride, and 100g of water are added to adjust the pH value to 6.6-7.0; 1.5kg is used according to the above ratio.

[0032] (2) Expanded cultivation: inoculate the seed culture solution into the expanded culture solution with an inoculation amount of 1.5%, and culture it on a shaker at 55-60° C. for 16-24 hours, with a rotating speed of 200-260 rpm;

[0033] Among them, the expanded culture...

Embodiment 2

[0038] Embodiment 2: the preparation two of composite biological decomposing agent:

[0039] a. Preparation of thermophilic Clostridium papyrus: (1) seed culture and (2) expanded culture are the same as in Example 1.

[0040] (3) Bacteria preparation: add 0.2kg of protective agent and 25kg of carrier adsorbent to the cultured product of step (2), and fully dry to obtain powdery bacterium;

[0041] Wherein, the protective agent is soybean protein and whey powder with a weight ratio of 3:1, and the carrier adsorbent is Jiayi powder.

[0042] b. The thermophilic Clostridium papyrus bacteria agent prepared in step a and other materials are prepared according to the following parts by weight: 5 parts of thermophilic Clostridium papyrus, 2 parts of thermophilic Lactobacillus, 20 parts The composite bacteria (the weight ratio of Bacillus subtilis, Pseudomonas, Bacillus pumilus is 8:1:1), the composite enzyme preparation of 2 parts (the weight of acid protease, endo-type cellulase, x...

Embodiment 3

[0044] Embodiment 3: the preparation control of thermophilic Clostridium papyrus

[0045] Referring to the preparation method of Example 1, no formaldehyde was added to the expanded culture medium for induction (uninduced group). In the microbial agent obtained under the same preparation conditions, the content of the thermophilic Clostridium papyrusi liquid that does not add formaldehyde to induce (uninduced group) is 3.5 billion / ml, and adds formaldehyde to induce (induced group). The content of Clostridium papyrus liquid is as high as 8.2 billion / ml. The thermophilic Clostridium papyrus produced by the two methods is now compared in the cellulose degradation experiment of reed straw.

[0046] Experimental method: 1. Mix 50% reed straw powder, 10% rice bran, 34% rice husk distiller's grains, 4% corn flour, 1% urea, 0.4% yeast powder, and 0.3% potassium dihydrogen carbonate It was reconstituted into a solid medium, and sterilized at 121°C for 30 minutes before use, and its ...

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Abstract

The invention discloses a composite biological decomposition agent which is composed of the following components in parts by weight: 2-10 parts of thermophilic cyperus papyriferus, 1-5 parts of lactobacillus thermophilus, 10-30 parts of composite bacteria, 2-5 parts of a composite enzyme preparation, 2-5 parts of composite actinomycetes, 5-10 parts of composite fungi, 40-70 parts of an organic carrier and 0.001 part of tea tree essential oil. The invention also discloses a preparation method of the composite biological decomposition agent. The composite biological decomposition agent can efficiently, quickly and thoroughly decompose organic materials, has the characteristics of high temperature rise speed and long high-temperature lasting time, can effectively kill pathogenic bacteria and worm eggs in the materials, greatly shortens the primary decomposing time, reduces the decomposing time by about one week, and ensures that the decomposed materials do not contain harmful bacteria; as the flora can generate spores and is resistant to high temperature, the flora can be greatly reserved after the materials are thoroughly decomposed, and the effect of the flora on soil and crops is exerted.

Description

technical field [0001] The invention relates to the technical field of organic waste treatment and recycling, in particular to a composite biological decomposing agent and a preparation method thereof. Background technique [0002] In recent years, with the excessive and unreasonable application of chemical fertilizers, the structure of the soil plow layer in my country has been severely damaged, the soil is barren, the soil fertility is low, and various crop diseases, insect pests and soil-borne diseases occur frequently, resulting in a large degree of crop yield and quality. are affected. From the perspective of soil fertilization, due to the continuous increase in the input of chemical fertilizers, the environmental pollution caused by it is becoming more and more serious. At the same time, due to the gradual loss of the input of organic fertilizers in traditional agriculture, the soil fertility has dropped significantly, and the content of soil organic matter has decrease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F17/20C05F17/80C12N1/20C12N1/14C12R1/145C12R1/225C12R1/01C12R1/645
CPCY02W30/40
Inventor 李建林薛万里张加明赵一
Owner 辽宁振兴生物科技发展有限公司