Fish-skin decellularized dermal matrix scaffold as well as preparation method and application thereof

A technology of decellularized dermis and matrix scaffold, which is applied in the field of fish skin decellularized dermal matrix scaffold and its preparation, can solve the problems of incomplete removal of DNA components, slow vascularization speed, poor hydrophilicity of ADM, etc., to improve the repair appearance, The effect of uniform and regular pores and strong hydrophilicity

Pending Publication Date: 2021-10-08
青岛蓝远生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the methods for preparing ADM cannot completely remove the DNA components, and the preparation process is cumbersome and time-consuming, and most ADMs are poor in hydrophilicity, which affects cell infiltration; too dense, resulting in slow vascularization; poor adhesion, resulting in skin grafts Difficult to survive, resulting in failure of self-skin grafting; too fast degradation, shrinkage of the wound surface in the later stage, etc.

Method used

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  • Fish-skin decellularized dermal matrix scaffold as well as preparation method and application thereof
  • Fish-skin decellularized dermal matrix scaffold as well as preparation method and application thereof
  • Fish-skin decellularized dermal matrix scaffold as well as preparation method and application thereof

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preparation example Construction

[0043] In a first aspect, the present invention provides a method for preparing a fish skin acellular dermal matrix scaffold, the method comprising the steps of:

[0044] 1) Process for preparing dermis slices: puffing the fish skin in an acid solution, removing the epidermis and subcutaneous tissue after puffing to obtain the dermis, and then de-puffing the dermis in a salt solution to obtain the dermis slices;

[0045] 2) Virus inactivation and decolorization process: soaking the dermis layer in a virus inactivation and decolorization solution to carry out virus inactivation and decolorization;

[0046] 3) Degreasing process: soaking the treated dermis in step 2) respectively in the alkali solution and the first surfactant solution to degrease;

[0047] 4) Immunogenicity removal process: immerse the treated dermis in step 3) respectively in hypotonic solution, trypsin solution and second surfactant solution to remove immunogenic substances; wherein, the hypotonic solution T...

Embodiment 1

[0109] This example is used to illustrate the fish skin acellular dermal matrix scaffold provided by the present invention and its preparation method

[0110] 1) Process for preparing dermal slices: take fresh snakehead skin, descale the snakehead skin by mechanical method, puff it with 0.05M acetic acid solution for 15 minutes, remove 0.2mm epidermis and subcutaneous tissue from the snakehead skin with an electric skin remover Finally, a dermis layer skin sheet with a thickness of 0.8mm is made, and the above-mentioned skin sheet is soaked in 0.5% by weight of potassium chloride solution for depuffing for 30min;

[0111] 2) Virus inactivation and decolorization process: soak the dermis layer in 1% by weight peracetic acid solution for 2 hours to carry out virus inactivation and decolorization treatment;

[0112] 3) Degreasing process: immerse the dermis layer obtained in step 2) in a 0.5% by weight sodium hydroxide solution for 1 hour, and soak in a 0.2% by weight TritonX-100...

Embodiment 2

[0119] This example is used to illustrate the fish skin acellular dermal matrix scaffold provided by the present invention and its preparation method

[0120]1) Process for preparing dermal slices: take fresh tilapia skin, descale the tilapia skin by mechanical method, puff it with 0.1M hydrochloric acid solution for 20 minutes, and remove the tilapia skin with an electric skin remover 0.25mm epidermis and subcutaneous tissue to make a dermis skin sheet with a thickness of 1.0mm, and soak the above-mentioned skin sheet in 1% by weight sodium chloride solution for depuffing for 60min;

[0121] 2) Virus inactivation and decolorization process: soak the dermis layer in 3% by weight hydrogen peroxide solution for 4 hours to carry out virus inactivation and decolorization treatment;

[0122] 3) Degreasing process: immerse the dermis slices obtained in step 2) in 1% by weight sodium carbonate solution and 1.5% by weight alkyl glucoside solution for degreasing, and the soaking time i...

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Abstract

The invention relates to the field of biomedical materials, in particular to a fish-skin decellularized dermal matrix scaffold as well as a preparation method and application thereof. The method comprises the following steps: pretreating fish skin to obtain dermal-layer skin grafts; and separately carrying out virus inactivation, decoloration, degreasing, immunogenicity removal, cross-linking, freeze-drying shaping, cutting and sterilization on the dermal-layer skin grafts. According to the method disclosed by the invention, the damage to a collagen structure of the fish-skin dermal matrix scaffold is reduced to the maximum, collagenous fibers are arranged in order, pores are uniform and regular, the hydrophilicity is strong, rapid vascularization can be realized, and good mechanical properties, good biocompatibility and good tissue induction capability are achieved. The material is widely applied to replacement and repair treatment of dermis defects.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a fish skin decellularized dermal matrix scaffold and its preparation method and application. The fish skin decellularized dermal matrix scaffold material can be used for replacement and repair of animal (emphasis on human) dermal defect tissue . Background technique [0002] The skin is the largest organ in the human body and has an extremely important barrier function: on the one hand, it prevents the loss of water, electrolytes, and other substances in the body; on the other hand, it prevents the invasion of harmful substances from the outside. When the superficial layer of skin or a small area of ​​skin is damaged, the body's own new skin will be regenerated. However, if a large area of ​​deep skin is traumatized, the skin cannot repair itself, and autologous skin transplantation is usually required at this time. Currently, as a dermal substitute for full-thickness skin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/36A61L27/50A61L27/56A61L2/18A61L2/08A61L101/22A61L101/32
CPCA61L27/60A61L27/3633A61L27/3687A61L27/3691A61L27/362A61L27/50A61L27/56A61L2/007A61L2/0088A61L2430/40A61L2202/21A61L2101/32A61L2101/02
Inventor 王园园李八方李青代元坤宋文山
Owner 青岛蓝远生物工程有限公司
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