Trichoderma harzianum microcapsule and preparation method thereof

A microcapsule and microcapsule suspension technology, applied in the field of microorganisms, can solve the problems of bacterial activity damage, high equipment requirements, and bacterial damage, and achieve the effects of good encapsulation, good size uniformity, and high embedding rate

Pending Publication Date: 2021-10-19
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The size uniformity of the microcapsules prepared by the emulsification method is poor, and vigorous mechanical stirring is required during the preparation process, which will greatly damage the activity of bacteria
The spray drying method requires short-term high-temperature

Method used

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  • Trichoderma harzianum microcapsule and preparation method thereof
  • Trichoderma harzianum microcapsule and preparation method thereof
  • Trichoderma harzianum microcapsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1

[0044] Preparation of Harzish Mildewele

[0045] 1, Preparation of Harz Mercine Suspended Suspension

[0046]Harzmushia bacteria is provided by the Department of Pesticide School of Plant Protection, Shenyang Agricultural University. After activation of Hanzum mold bacteria, liquid fermentation culture, the specific components are as follows, corn flour 63g, glycerol 7.5ml, start pH 4.0, distilled water 1000ml . Divided into a 500 ml cone flask, 150 ml per bottle of liquid, at 28 ° C for 144 h at 28 ° C, centrifuged after filtration, collected, and a small amount of physiological saline was mixed. Test the number of live bacteria in the fungus suspension by tablet counting, to ensure that the number of live bacteria is 1 × 10 10 CFU / mL, placed at 4 ° C refrigerator preservation spare.

[0047] 2, Harzli microcapsules preparation order factor conditions optimization

[0048] 2.1 The effect of wall material on microcapsules

[0049] Take 1 × 10 9 CFU / ML Harz M...

Example Embodiment

[0086] Example 2

[0087] Determination of performance indicators of Harzwool microcapsules

[0088] 1, experimental method

[0089] 1.1 microcapsule particle size measurement method

[0090] The particle diameter section and the median division of the microcapsules were measured by the BT-9300H laser particle size distribution, and a small amount of microcapsules were placed in a stirrer containing distilled water, and the microcapsules were tested for 3 min, and the particle size was measured. .

[0091] 1.2 microcapsules morphological characteristic observation method

[0092] The sub-microcapsules were suspended by a microcapsule (1DIV = 0.01 mm), and the form of microcapsules was observed under 10x10 times with an optical microscope using an optical microscope. The surface of the microcapsules was observed on the SEM sample table, and the scanning electron microscope acceleration voltage was 5 kV.

[0093] 1.3 Harzli microcapsules storage stability determination

[0094] Afte...

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PUM

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Abstract

The invention discloses a trichoderma harzianum microcapsule and a preparation method thereof, belonging to the technical field of microorganisms. The trichoderma harzianum microcapsule is successfully prepared by taking Arabic gum and gelatin as wall materials through a complex coacervation method. The particle size of the microcapsule prepared by the method is mainly in a range of 20-40 [mu] m, and size uniformity is good; the wall materials have a good wrapping effect on a core material, and an embedding rate is high; the survival rate of spores is 83.34% after the spores are stored at 4 DEG C for 720 days; and the survival rate of the spores at room temperature is 86.11%, and a good protection effect on microorganisms is achieved. The optimal microcapsule preparation method is determined through a single factor experiment and response surface optimization, and the method is easy to operate, low in cost, short in technological process and suitable for industrial application and popularization. The invention develops a novel environment-friendly pesticide formulation of trichoderma harzianum, which has important significance for further popularization and application of trichoderma harzianum.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma harzianum microcapsule and a preparation method thereof. Background technique [0002] Trichoderma harzianum is the most widely used strain in the Trichoderma genus. Whether it is in agriculture or forestry, Trichoderma harzianum is widely used to control various plant diseases, and its biocontrol effect has been verified in many application tests. At present, the application dosage forms of Trichoderma harzianum mainly include wettable powder, water dispersible granule and so on. [0003] Wang Xigang et al. (2020) disclosed the composition and ratio of Trichoderma harzianum M-17 chlamydospore wettable powder, wherein chlamydospore powder is 20%, the carrier is 67% attapulgite, and the wetting agent is 5% chlamydospore. Wen-40, the dispersant is 7% sodium carboxymethyl cellulose, and the UV protective agent is 1% vitamin C. Niu Senlin et al. (2020) determin...

Claims

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Application Information

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IPC IPC(8): A01N63/38A01N25/28A01P3/00B01J13/14
CPCA01N63/38A01N25/28B01J13/14
Inventor 何璐孟军殷志鹏刘睿博慈峻哲陈胜男
Owner SHENYANG AGRI UNIV
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