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Trichoderma harzianum microcapsule and preparation method thereof

A microcapsule and microcapsule suspension technology, applied in the field of microorganisms, can solve the problems of bacterial activity damage, high equipment requirements, and bacterial damage, and achieve the effects of good encapsulation, good size uniformity, and high embedding rate

Pending Publication Date: 2021-10-19
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The size uniformity of the microcapsules prepared by the emulsification method is poor, and vigorous mechanical stirring is required during the preparation process, which will greatly damage the activity of bacteria
The spray drying method requires short-term high-temperature drying, which will inevitably cause great damage to the bacteria, and has relatively high requirements for equipment.
[0007] At present, there is still a lack of a preparation method of Trichoderma harzianum microcapsules with simple preparation method and good product quality.

Method used

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  • Trichoderma harzianum microcapsule and preparation method thereof
  • Trichoderma harzianum microcapsule and preparation method thereof
  • Trichoderma harzianum microcapsule and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0044] Preparation of Trichoderma harzianum microcapsules

[0045] 1. Preparation of Trichoderma harzianum spore suspension

[0046]The strains of Trichoderma harzianum were provided by the Pesticide Teaching and Research Group of the Plant Protection College of Shenyang Agricultural University. After the activation of Trichoderma harzianum strains, liquid fermentation culture was carried out. The specific components are as follows: corn flour 63g, glycerin 7.5mL, initial pH 4.0, distilled water 1000mL . Pack into 500mL Erlenmeyer flasks, the volume of each bottle is 150mL, incubate at 28°C at 150r / min for 144h, filter and centrifuge, collect the sludge, add a small amount of normal saline and mix well. The number of viable bacteria in the bacterial suspension was detected by plate counting method, and the number of viable bacteria was guaranteed to be 1×10 10 cfu / mL, store in a 4°C refrigerator for later use.

[0047] 2. Optimization of single factor conditions for the pre...

Embodiment 2

[0087] Determination of Performance Indexes of Trichoderma harzianum Microcapsules

[0088] 1. Experimental method

[0089] 1.1 Determination method of microcapsule particle size

[0090] Use the BT-9300H laser particle size distribution analyzer to measure the particle size range and median diameter of the microcapsules, put a small amount of microcapsules in a stirrer filled with distilled water, ultrasonically treat for 3 minutes, and measure the particle size after the microcapsules are evenly dispersed in water .

[0091] 1.2 Observation method of morphological characteristics of microcapsules

[0092] Drop 1 drop of the prepared microcapsule suspension onto a glass slide with a micrometer (1 DIV=0.01mm), and observe the morphology of the microcapsules with an optical microscope at 10×10 times. The prepared microcapsule powder was stuck on the SEM sample stage to observe the surface morphology of the microcapsules, and the accelerating voltage of the scanning electron ...

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Abstract

The invention discloses a trichoderma harzianum microcapsule and a preparation method thereof, belonging to the technical field of microorganisms. The trichoderma harzianum microcapsule is successfully prepared by taking Arabic gum and gelatin as wall materials through a complex coacervation method. The particle size of the microcapsule prepared by the method is mainly in a range of 20-40 [mu] m, and size uniformity is good; the wall materials have a good wrapping effect on a core material, and an embedding rate is high; the survival rate of spores is 83.34% after the spores are stored at 4 DEG C for 720 days; and the survival rate of the spores at room temperature is 86.11%, and a good protection effect on microorganisms is achieved. The optimal microcapsule preparation method is determined through a single factor experiment and response surface optimization, and the method is easy to operate, low in cost, short in technological process and suitable for industrial application and popularization. The invention develops a novel environment-friendly pesticide formulation of trichoderma harzianum, which has important significance for further popularization and application of trichoderma harzianum.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma harzianum microcapsule and a preparation method thereof. Background technique [0002] Trichoderma harzianum is the most widely used strain in the Trichoderma genus. Whether it is in agriculture or forestry, Trichoderma harzianum is widely used to control various plant diseases, and its biocontrol effect has been verified in many application tests. At present, the application dosage forms of Trichoderma harzianum mainly include wettable powder, water dispersible granule and so on. [0003] Wang Xigang et al. (2020) disclosed the composition and ratio of Trichoderma harzianum M-17 chlamydospore wettable powder, wherein chlamydospore powder is 20%, the carrier is 67% attapulgite, and the wetting agent is 5% chlamydospore. Wen-40, the dispersant is 7% sodium carboxymethyl cellulose, and the UV protective agent is 1% vitamin C. Niu Senlin et al. (2020) determin...

Claims

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Application Information

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IPC IPC(8): A01N63/38A01N25/28A01P3/00B01J13/14
CPCA01N63/38A01N25/28B01J13/14
Inventor 何璐孟军殷志鹏刘睿博慈峻哲陈胜男
Owner SHENYANG AGRI UNIV
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