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A kind of degradable superfibroin-based material and its preparation method and application

A silk fibroin and base material technology, applied in the direction of single-component silk fibroin artificial filament, rayon manufacturing, textile and paper making, etc. Simple and easy to operate effect

Active Publication Date: 2022-07-29
WESTLAKE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made through previous related research, until now, there is still a lack of very effective and easy processing and preparation methods

Method used

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  • A kind of degradable superfibroin-based material and its preparation method and application
  • A kind of degradable superfibroin-based material and its preparation method and application
  • A kind of degradable superfibroin-based material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0056] Degradable super tenacin films were prepared according to the following method:

[0057] (1) As in Table 1, prepare silk protein solution, glycerol solution, and calcium chloride solution respectively, and mix the above solutions according to the volume in Table 1 after the configuration is completed. Take 3.5mL of the mixed solution and pour it into a petri dish with a diameter of 6mm, so that the solution completely covers the surface of the petri dish.

[0058] (2) placing the open mouth of the culture dish on which the mixed solution was poured to be dried at room temperature for 12 hours, and then placing the dried culture dish in a vacuum drying oven to continue drying at room temperature for 2-4 hours to prepare each silk protein film ( 15% glycerol + 5% calcium chloride; 15% glycerol + 10% calcium chloride; 20% glycerol + 5% calcium chloride; 20% glycerol + 10% calcium chloride).

[0059] The photo of the prepared silk protein film (20% glycerol + 10% calcium c...

Embodiment 3

[0079] The degradation properties of the films obtained in Example 3 were characterized by their degradation performance in different enzyme solutions (Protease XIV, collagenase and α-chymotrypsin). The samples were cut into 10mm×10mm squares and immersed in 2mL of different enzyme solutions respectively. The remaining sample mass was weighed on day 1 and day 3, respectively, and the degradation rate of the sample in different enzyme solutions could be calculated. Enzymatic degradation test results such as Image 6 shown.

[0080] Analysis of the results: from figure 2 and image 3 From the data in , it can be seen that with the increase of the proportion of glycerol added, the β-sheet content in the degradable super tenasin film also increases, and the glass transition temperature of the silk protein molecule decreases.

[0081] from Figure 4 It can be seen from the data in , that the elongation at break (stretchability) of the degradable super tenasin film increases w...

Embodiment 5

[0089] The low-concentration silk protein / glycerol / calcium chloride mixed aqueous solution prepared in Example 3 was concentrated to prepare a high-concentration mixed silk protein aqueous solution. The concentrated silk protein aqueous solution can be stably stored at low temperature after being purified by centrifugation, and the concentration is 40%. Figure 7 A diagram of a high concentration silk protein / glycerol / calcium chloride concentrate.

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Abstract

The present invention relates to a degradable superfibroin-based material and a preparation method and application thereof. The degradable superfibroin-based material of the present invention comprises a plasticizer, a salt and silk protein, the plasticizer is one or more selected from glycerol, sorbitol, polyethylene glycol, and the salt is one or more selected from calcium chloride, lithium chloride and lithium bromide. By adjusting the ratio of plasticizer to salt in the super fibroin-based material, the super fibroin-based material of the present invention has adjustable mechanical properties, degradation properties and water solubility, and can be developed into various Raw materials for bioengineering materials.

Description

technical field [0001] The invention relates to a silk protein-based material, in particular to a degradable super tough silk protein material, a preparation method and application thereof. Background technique [0002] Silk protein is a protein-based biopolymer extracted from natural silkworm cocoons with excellent mechanical properties, biocompatibility and biodegradability. In recent years, silk protein-based materials have been widely used in biomedical fields such as tissue engineering, bioelectronics, and regenerative medicine. For example, silk protein films can be used as substrates for the preparation of biosensors; silk protein sponges can be used as scaffolds for tissue engineering; silk protein nanospheres can be used as carriers for drug delivery and release; silk protein blocks can be processed into implantable bone nails Wait. Generally, silk protein can be extracted from natural silkworm cocoons by the method of dissolution and regeneration. This preparatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08K3/16C08K5/053C08J5/18D01F4/02D01F1/10
CPCC08K3/16C08K5/053C08J5/18D01F4/02D01F1/10C08J2389/00C08K2003/162C08L89/00
Inventor 郭成辰吕浩
Owner WESTLAKE UNIV
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