Microbial source bactericidal ointment containing Xcn1 as well as preparation method and application of microbial source bactericidal ointment
A microbial source, xcn1 technology, applied in the field of preparation of microbial source bactericidal ointment, to achieve the effect of simple configuration method, reduced impact, and reduced use
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Embodiment 1
[0043] Example 1: Preparation of Xcn1 high-concentration parent drug (for details, see "Xenorhabdusnematophila YL001 Medium Screening and Culture Condition Optimization", Wang Yonghong, Zhang Xing, 2006, "Journal of Northwest A&F University (Natural Science Edition)")
[0044] This embodiment provides the preparation method of 3.0% Xcn1 parent drug, it is that X.nematophila YL001 bacterial strain is placed in LB medium (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl), at 180rpm / min, 28 10% of the seed liquid was added to PP3 medium (2.0% peptone), fermented at 150rpm / min and 28°C for 48h, and the fermented liquid was fermented at 4°C and 10000rpm / Centrifuge for 20 min, collect the supernatant, add 5.5 g / L of aluminum sulfate octadecahydrate, stir rapidly for 1 min, place it for 6 h, centrifuge, take the supernatant, concentrate under reduced pressure to 1 / 4 of the original volume, add 80% Stir with ethanol for 10 min, refrigerate overnight at 4°C, centrifuge, take the supernata...
Embodiment 2
[0045] Example 2: Screening of additives and dosage during Xcn1 ointment processing
[0046] This example provides a screening method for additives and usage amounts during the processing of Xcn1 ointment. The types and dosages of film-forming agents, thickeners, moisturizers, and penetration enhancers involved in the Xcn1 ointment processing process are obtained through multiple screenings. of. The optimal concentration screened by single factor test was used as the central point, and a central combination test of four factors and three levels was designed, and the diameter of the inhibition zone of Xcn1 ointment against P. Optimum concentration of the agent used: polyvinyl alcohol 5.32%, sodium carboxymethyl cellulose 2.52%, azone 2.46%, glycerol 1.96%.
[0047] Screening of film-forming agent and its dosage: film-forming materials include sodium carboxymethyl cellulose, polyvinyl alcohol, polyethylene glycol, organic bentonite, diatomaceous earth, sodium alginate, liquid p...
Embodiment 3
[0053] Embodiment 3: Preparation of 0.08% Xcn1 ointment
[0054] This example provides the preparation method of 0.08% Xcn1 ointment, which is to add 5.32% polyvinyl alcohol to an appropriate amount of water, heat to dissolve at 90°C, pass through a 40-mesh sieve to discard the insoluble matter, and after cooling to 65°C, add 2.52% 2.46% Azone and 1.96% Glycerol were added to the above mixture, mixed evenly and cooled to 45°C, 2.67mL 3.0% Xcn1 concentrated fermentation broth was added, and water was added to 100%, stirring at a stirring speed of 600-800 rpm for 20-30 minutes, can prepare 0.08% Xcn1 ointment. The method is simple and easy, low in cost and suitable for mass production.
[0055] The preparation method of 0.04%, 0.06%, 0.15% Xcn1 ointment is the same as that of 0.08% Xcn1 ointment, only the volume of 3.0% Xcn1 concentrated solution is added.
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