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Mesona chinensis polysaccharide gel ball as well as preparation method and application thereof

A technology of fairy grass polysaccharide and gel ball, which is applied in food science and other directions, can solve the problems of not achieving the improvement of gastric acid stability and intestinal targeted release effect of probiotics or embedded components, so as to improve the absorption capacity of the small intestine and improve the resistance The effect of gastric acid capacity and high embedding stability

Active Publication Date: 2022-04-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The publication number is CN 111357817 A, titled "An active health-preserving yogurt jelly jelly and its preparation method". The jelly jelly product with antioxidant effect is obtained by compounding jelly powder and yogurt, but the jelly polysaccharide-protein compound Gel or jelly did not achieve improved gastric acid stability and intestinal targeted release of probiotics or entrapped ingredients

Method used

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  • Mesona chinensis polysaccharide gel ball as well as preparation method and application thereof
  • Mesona chinensis polysaccharide gel ball as well as preparation method and application thereof
  • Mesona chinensis polysaccharide gel ball as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 High Performance Liquid Chromatography Determination of Jelly Grass Polysaccharide Composition

[0070] Specific steps are as follows:

[0071] (1) Preparation of the sample loading solution for the jellybean polysaccharide sample to be tested:

[0072] Grass jelly polysaccharide was purchased from Wuhan Yuancheng Gongchuang Technology Co., Ltd. (CAS: 542-40-5, standard: enterprise standard, grade: food grade, content: 99%). Put 10 mg of grass polysaccharide sample into a 10ml ampoule, add 2.0mL 2 mol / L trifluoroacetic acid into a 5 mL ampoule, seal the tube, acidolyze at 110°C for 8 hours, take out and evaporate the trifluoroacetic acid, add 2.0ml water to redissolve.

[0073] Sample solution derivation: Accurately pipette 250uL sample solution into a 5mLEP tube, add 250uL 0.6 mol / L NaOH, 500 uL 0.4mol / L 1-phenyl-3-methyl-5-pyrazolone-methanol, react at 70°C for 1h . Cool in cold water for 10 minutes; add 500 uL of 0.3mol / L HCl to neutralize, then add 1 mL...

Embodiment 2

[0081] Example 2 Preparation of Jelly Grass Microgel Balls by Inverse Emulsion Method

[0082] Accurately weigh 50 mg jellybean polysaccharide, dissolve it in 0.5 mL of pH3 water, vortex until the polysaccharide is completely dissolved; accurately weigh 5 mg ferric chloride metal ion. Mix jelly polysaccharide solution with metal ions.

[0083]Weigh 40 g of liquid paraffin and 1 g of Span80 into a 50 mL beaker, add a mixture of polysaccharide and iron that is completely mixed, and stir magnetically for 1 h to fully crosslink the polysaccharide and metal ions.

[0084] After 1 h, centrifuge at 1000 rpm for 2 min, separate the oil phase from the water phase, discard the upper oil phase, and wash the microspheres in the lower water phase with n-hexane and methanol for 3 times, and finally redissolve the microgel balls to 1 mL pH3 in distilled water.

[0085] The Grass jelly microgel balls were scanned by scanning electron microscope SEM. figure 2 It was shown that the jelly gr...

Embodiment 3

[0087] Example 3 Preparation of Gel Microspheres Embedding Probiotics

[0088] 0.5 mL6×10 10 Cfu / mL probiotics were centrifuged at 3000 rpm at 4°C for 10 min, washed twice with normal saline, and redissolved in 0.5 mL of normal saline. Dissolve 50 mg of jellybean polysaccharide in 0.5 mL of pH3 water, vortex until the polysaccharide is completely dissolved; accurately weigh 5 mg of ferric chloride, fully mix jellybean polysaccharide solution, bacterial solution and metal ions, and add 40 g of liquid paraffin and 1 g Span80 in a 50 mL beaker, magnetically stirred for 1 h to fully cross-link the polysaccharides with metal ions, centrifuged at 1000 rpm for 2 min after 1 h, separated the oil phase from the water phase, discarded the upper oil phase, and used n-hexane for the lower water phase. The microspheres were washed three times with alkane and methanol respectively, and finally the microgel spheres embedded with probiotics were redissolved in 1 mL of distilled water at pH ...

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Abstract

According to the mesona chinensis benth polysaccharide gel ball and the preparation method and application thereof, the circular gel ball formed by mesona chinensis benth polysaccharide has good gastric acid resistance, the stability of active ingredients in gastric acid is effectively protected, intestinal targeted release of the active ingredients in intestinal tracts can be achieved, the utilization rate of probiotics or functional ingredients is effectively increased, and the bioavailability of the mesona chinensis benth polysaccharide gel ball is improved. The probiotics are helped to adhere to intestinal mucus and colonize in intestinal tracts for a long time, and the intestinal release and absorption capacity of other active ingredients is improved.

Description

technical field [0001] The invention relates to the field of food biotechnology. Specifically, the present invention relates to jelly jelly polysaccharide gel ball and its preparation method and application. Background technique [0002] Grass jelly has another name called fairy grass, jelly grass, the scientific name is Mesonachinensis Benth, and the English name is Mesona Blume. It is produced in Taiwan, Fujian, Zhejiang, Jiangxi, Guangdong, Guangxi and other places. It is a medicinal and edible plant rich in jelly jelly polysaccharide ( jelly jelly) and flavonoids. It is an ideal raw material for the production of natural, nutritious and health food, and has a wide range of processing and utilization values. Grass jelly is astringent, sweet, and cold in nature and has the effect of clearing heat and quenching thirst, cooling blood and relieving heat, and can cure heatstroke, high blood pressure, kidney disease, and diabetes. Foods processed with jelly grass have been re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L29/206
CPCA23L29/206A23L29/294A23P10/30A23L33/105A23L33/15A23L33/10A23L33/19A23L33/17A23L33/135A23L2/52A23C9/1307A23C9/1322A23C19/09A23C19/0921A61K9/06A61K47/46A23V2002/00A23V2200/30A23V2250/51A23V2250/1592A23V2200/3204A23V2250/2112A23V2250/21
Inventor 李媛张慧娟刘斌王启蒙李星侯国华梁爽闫恵苓
Owner CHINA AGRI UNIV
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