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Preparation method of compound bacterium microcapsule for treating salinization and promoting growth of cotton

A technology of microcapsules and composite bacteria, applied in the direction of microcapsule preparations, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as interference with normal plant metabolism, water balance breakdown, plant cell toxicity, etc., to protect viability , Conditioning soil alkalinity, good slow-release effect

Pending Publication Date: 2022-05-13
万之源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Second, the Na in the cell ＋ and Cl － After the concentration of the plant is increased, the plant cells will have a toxic effect, inhibit the activity of the enzyme, interfere with the normal metabolism of the plant, and poison the plant.
At the same time, when plants grow in salty soil under salt stress, the problem they face is that the original water balance is broken.

Method used

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  • Preparation method of compound bacterium microcapsule for treating salinization and promoting growth of cotton
  • Preparation method of compound bacterium microcapsule for treating salinization and promoting growth of cotton
  • Preparation method of compound bacterium microcapsule for treating salinization and promoting growth of cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The present embodiment controls the preparation method of the compound bacteria microcapsules that salinization promotes the growth of cotton, comprises following main steps:

[0052] (1) Preparation of the main material of the microcapsule skeleton

[0053] 1.1 The cotton stalks are pulverized, deashed, dewaxed and dried to obtain cotton stalk powder; the cotton stalks in this embodiment are agricultural waste cotton stalks;

[0054] in,

[0055] 1.11 After the cotton stalks are pulverized by a pulverizer, the obtained waste cotton stalks are crushed into powder and sent to the deashing device for deashing;

[0056] 1.12 Cook with absolute ethanol for 2 hours for dewaxing, and rinse with deionized water;

[0057] 1.13 Place the material obtained in step 1.12 in an oven and dry at 105°C for 12 hours;

[0058] 1.2 Feed 60% sulfuric acid, cotton stalk powder and γ-valerolactone at a ratio of 1:10:100, mix evenly, stir at 80°C for 1 hour, then filter at the same tempera...

Embodiment 2

[0083] The difference between this embodiment and embodiment 1 is that

[0084] Step 1.13, drying at 100°C for 10 hours;

[0085] Step 1.2, 150g of cotton stalk powder, stirred at 90°C for 1.5h;

[0086] Step 1.3, the filter residue of step 1.2 was boiled with deionized water for 2.5 hours, added with sodium hydroxide solution and boiled for 2.5 hours;

[0087] Step 1.4, mix the slurry with sodium hypochlorite and hydrogen peroxide, stir at 45°C for 40 minutes, and acidify for 12 minutes;

[0088] Step 1.5, after mixing the filter residue and sodium hydroxide solution, stir and react at 30°C for 40 minutes. The temperature of the autoclave is 230°C;

[0089] Step 2.2, the culture substrate includes 30g glucose, 30g hemicellulose and 150g sodium alginate;

[0090] Step 2.3, Rs-198 strain 10g, Pseudomonas chloropinus R5 strain 10g, Bacillus subtilis GB03 strain 10g;

[0091] Steps 2.31 to 2.33, pH control is 7.0;

[0092] Step 3.1, the temperature of the water bath is kept ...

Embodiment 3

[0098]The difference between this embodiment and embodiment 1 is that

[0099] Step 1.13, drying at 110°C for 12 hours;

[0100] Step 1.2, 200g of cotton stalk powder, stirred at 100°C for 1h;

[0101] Step 1.3, the filter residue of step 1.2 was boiled with deionized water for 1.5h, added with sodium hydroxide solution and boiled for 1.5h;

[0102] Step 1.4, mixing the slurry with sodium hypochlorite and hydrogen peroxide and stirring at 35°C for 60 minutes;

[0103] Step 1.5, the temperature of the autoclave is 225°C;

[0104] Step 2.2, the culture substrate includes 40g glucose, 40g hemicellulose and 200g sodium alginate;

[0105] Step 2.3, Rs-198 strain 15g, Pseudomonas chloropinus R5 strain 15g, Bacillus subtilis GB03 strain 15g;

[0106] Steps 2.31 to 2.33, pH control is 7.5;

[0107] Step 3.1, keep the temperature of the water bath at 80°C;

[0108] Step 3.2, constant temperature ultrasonic dispersion at 55°C for 45 minutes. After centrifugal washing, dry at 90°C...

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Abstract

The invention relates to a preparation method of a compound bacterium microcapsule for treating salinization and promoting cotton growth. The preparation method comprises the following steps: (1) preparing a microcapsule framework main material; (2) constructing a composite flora system; (3) preparing a hollow microcapsule; and (4) preparing the compound bacterium microcapsule. In the step (1), cellulose is extracted from cotton stalks, a microcapsule framework material is prepared, agricultural waste cotton stalks are fully recycled, the method is green and environmentally friendly, the cost is saved, the prepared microcapsules are good in slow release effect, and the composite flora microorganisms prepared through the preparation method can adjust the alkalinity of saline-alkali soil and can promote cotton growth; the survival rate of the cotton under the salt stress is enhanced. According to the invention, Rs-198 bacteria, pseudomonas chlororaphis R5 bacteria and bacillus subtilis GB03 are prepared into a composite flora, and compared with a single strain, the prepared composite flora has the effect of 1 + 1 > 2 through a synergistic effect in conditioning saline-alkali soil.

Description

technical field [0001] The invention relates to the technical field of cotton planting, in particular to a method for preparing a compound bacterial microcapsule for controlling salinization and promoting cotton growth. Background technique [0002] Cotton is an important strategic material of the country. There are about 45 million mu of cotton planted in Xinjiang, which is the most suitable cotton area and the largest production base of high-quality commercial cotton in my country. 1.4 million hectares of cotton planted each year 2 above. In recent years, due to the large amount of evaporation and the large-scale application of drip irrigation technology, the secondary salinization of soil in Xinjiang has been aggravated to a certain extent, making salt stress an important factor affecting the high and stable yield of cotton in Xinjiang. Researchers collectively refer to the limiting factors of saline-alkali soil on plant growth as salt stress. Salt stress mainly inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04B01J13/04C09K17/14A01N63/27A01N63/22A01N25/28A01P21/00C09K101/00C09K109/00C12R1/125C12R1/38C12R1/39
CPCC12N1/20C12N1/04B01J13/043C09K17/14A01N63/27A01N63/22A01N25/28C09K2101/00C09K2109/00
Inventor 杜荣蔡宏曾松江汪远昊
Owner 万之源生物科技有限公司
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