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Method for extracting natural abscisic acid

A technology of natural abscisic acid and extraction method, which is applied in the direction of fermentation, can solve the problems of unsuitability for industrial production, and achieve the effects of simple and reasonable process, low discharge of three wastes, and low investment

Active Publication Date: 2006-03-22
江西新瑞丰生化股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product yield disclosed in JP-A-3-6139 is 73%, which is not suitable for industrialized production

Method used

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  • Method for extracting natural abscisic acid
  • Method for extracting natural abscisic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 6000mL fermentation medium ingredients: 5.0% dextrin, 0.3% wheat bran, 0.02% ethylene glycol, 2.0% glucose, 3.0% sucrose, 1.0% bean cake powder, 0.01% dipotassium hydrogen phosphate, 0.05% magnesium sulfate,

[0033] Divide the above medium into 20 1000mL Erlenmeyer flasks, 300mL in each bottle, sterilize at 120°C for 30 minutes, inoculate the spore suspension of activated abscisic acid high-yielding strain Botrytis cinerea TBI-9 after cooling, at 23-29°C Carry out fermentation culture. Oscillation speed 150rpm, amplitude 20cm. The fermentation time is 8-9 days, and the concentration of natural abscisic acid reaches 0.12%.

[0034] Take 5000 grams of fermentation broth (the content of natural abscisic acid is 6.025 grams), add 3N sulfuric acid solution to adjust the Ph to 1.5-5.0, and carry out acidification treatment for 30 minutes. Filter with filter paper at room temperature to remove bacteria and impurities in the fermentation broth, use 1000 grams of 101 macropor...

Embodiment 2

[0039] The preparation method of fermented liquid is the same as that of Example 1. Get 5000 grams of fermented liquid (the content of natural abscisic acid is 6.010 grams), add 4.5 liters of water, make the concentration of natural abscisic acid in the liquid be 0.012%, and use this solution to replace the original fermented liquid for extraction separate. Add 3N hydrochloric acid solution to adjust the Ph to 1.5-5.0, centrifuge at 9000 rpm for 10 minutes at room temperature, and collect the supernatant. After the centrifuged supernatant was contacted with 1000 grams of 101 macroporous cation exchange resin for 1 hour, the resin was washed with 2500 mL of 40-85% methanol for 30 minutes, and the methanol solution containing natural abscisic acid was collected. The natural abscisic acid content of the methanol solution was 5.920 grams.

[0040]The eluting methanol liquid is distilled under reduced pressure on a Buchi R-3000 rotary distillation device, the vacuum degree is kept...

Embodiment 3

[0044] The preparation method of fermented liquid is the same as embodiment 1, gets 5000 grams of fermented liquid (natural abscisic acid content is 6.110 grams). In the extraction and separation operation, the natural abscisic acid adsorbed by the resin is eluted with ethanol instead of methanol after the 101 macroporous cation exchange resin is contacted with the natural abscisic acid in the enriched acidified liquid, and the other operations are basically the same as in Example 1. . Finally, 5.589 grams of natural abscisic acid products with a purity of 98.5% were obtained, and the product yield was 90.1%.

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Abstract

The present invention belongs to the field of biological engineering, and discloses natural abscisic acid extracting method. Through acidifying fermented liquid, filtering, adsorption with macroporous adsorption resin, elution, concentrating and extracting the eluted liquid, concentrating the extracted liquid to obtain coarse crystallized product, dissolving the coarse crystallized product, adsorbing impurity with silicon gel, adding hexane, benzene, petroleum ether or carbon tetrachloride as crystallization assistant, decompression concentration, cooling to crystallize, washing, and vacuum drying, crystalline natural abscisic acid of purity over 95 % is produced. The method has high extracting rate, high abscisic acid product purity and other advantages.

Description

[0001] 1. Field [0002] The invention belongs to the field of biochemical industry, in particular to the field of extraction and separation of natural substances. 2. Background technology [0003] Abscisic acid is one of the five major plant hormones and an important growth regulator of plants. It can promote the maturity and development of fruits, grains, beans, etc., greatly improve their yield and quality, and greatly enhance their cold resistance, drought resistance and salt resistance. Alkaline capacity, so it has broad application prospects. [0004] The optical configuration of natural abscisic acid in plants is (s)-(+)-abscisic acid, and its structural formula is: [0005] [0006] 2-cis-(+)-abscisic acid [0007] A method of grinding plant cells, extracting with organic solvents, extracting with sodium bicarbonate aqueous solution, concentrating the extract, and then performing column chromatography to direct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40
Inventor 周金燕谭红杨杰李志东
Owner 江西新瑞丰生化股份有限公司
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