Methods for producing multilayered particles, fibers and sprays and methods for administering the same
a multi-layered particle and fiber technology, applied in the direction of microcapsules, chemical/physical processes, drug compositions, etc., can solve the problems of insufficient tumor distribution or therapeutic agents, and infallible breast cancer early detection. , to achieve the effect of low and acceptable levels, less manufacturing steps, and cost-effective
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specific example 1
[0156] This example described an encapsulation of a protein solution using a modification of the shell formulation that consisted of a solution of bovine serum albumin (BSA) in the presence of salts such as phosphates to stabilize the pH of the solution. Confocal fluorescence microscopy (CFM) and BSA with a fluorescent tag was used for visualizing the capsule. About 2 mg fluorescent protein was dissolved in about 1 mL of phosphate-buffer saline (PBS), type buffer that stabilized the acid-base properties of this aqueous solution to a pH equal to about 7.4.
[0157] The final BSA concentration was adjusted to about 3 μM. Silica sol was used as the shell fluid precursor following the sol aging procedures, with addition of tert-amyl alcohol to a 50:50 volume ratio.
[0158] In general, for solution aging purposes, an acidified tetraethyl orthosilicate solution in ethanol may be aged at about 80° C. for about 4 to about 6 hours. Ter-amyl alcohol was added to increase the hydrophobicity of th...
specific example 2
[0160] The sol-gel methods and process variables used to encapsulate fluorescent BSA in Specific Example 1 were slightly modified to encapsulate a transaminase. This enzyme was used to catalyze the following reaction:
D.L glutamine+glyoxylic acid→L-glutamine (left unreacted)+α-keto derivative (from D-glutamine)-glycine
[0161] Each enantiomeric from has the ability to rotate polarized light, and a technique known as polarimetry may be used to follow chemical reactions in involving enantiomers as a function of time. The reactants, on the left hand side of the reaction shown above, are basically a mixture with no optical activity, since the D,L prefix stands for about a 50:50 mixture of the D and L enantiomers of glutamine. The products, on the right hand side of the reaction shown above, become enriched in unreacted L-glutamine with a concomitant time-dependent optical rotation signal that may be tracked by polarimetry, because this particular transaminase only catalyzes reactions invo...
specific example 3
particles with 3.1 LACZ as DNA Marker
[0164] The therapeutic solution was prepared by mixing 3.1 LACZ in about 10 mM Bis-Tris propane buffer aqueous solution containing about 1 wt % of isopropanol and about 2 mM of CaCl2. The final concentration of 3.1 LACZ was 700 μg / mL. A biopolymer solution was prepared by mixing two functionalized biopolymers in chloroform: (a) COOH-poly(ethylene glycol)-b-polylactide, or PEG-b-PLA, with a molecular weight of 2000-b-1940 Da using same nomenclature, respectively, and a Mw / Mn=1.2; and (b) Poly(caprolactone)-SH, or PCSH, with a molecular weight of 5,000 Da and a Mw / Mn=1.5. This solution was doped with a solution of magnetite particles with an average diameter of about 15 nm. The weight percent contents of PEG-b-PLA, PCSH, and magnetite particles in this solution were about 0.071 wt %, about 0.058 wt %, and about 0.004 wt % respectively.
[0165] The therapeutic and biopolymer solutions were mixed and dimethyl sulfoxide, or DMSO, was added to form a h...
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