The invention discloses a biosafety asepsis room with a monitoring function. The biosafety asepsis room with the monitoring function comprises an aseptic processing room, an air supply pipe, an air returning pipe and a purifier, wherein the purifier is placed in the air sending pipe. An air inlet of the air supply pipe is communicated outdoor, an air outlet of the supply pipe is communicated to the aseptic processing room, and an air returning inlet of the aseptic processing room is communicated to the air inlet of the air supply pipe. The purifier comprises a low efficient filter, a cooling tower, a heater, a draught fan, a medium efficiency filter and a plasma sterilizer placed in the gas flowing direction in sequence, and a monitoring upper computer used for monitoring the plasma sterilizer, and the plasma sterilizer detects current, voltage, density and electron energy of the plasma, and monitors whether a purifying unit is in a working state or not in real time. According to the biosafety asepsis room with the monitoring function, firstly, large particles in the air are firstly filtered out, then sterilization is conducted through a plasma method, thus it is guaranteed that recirculating air in the asepsis room is sterile and nontoxic, and the demand of many bio-pharmaceutical enterprises for biosafety grade asepsis room is met.