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Method for preparing bioactive poly(lactic-co-glycolic acid)/collagen/hydroxyapatite composite fiber membrane for bone repair

A technology of composite fiber membrane and hydroxyapatite, which is applied in medical science, prosthesis, etc., can solve the problems of poor mechanical properties and achieve the effects of excellent comprehensive performance, good biocompatibility, and wide source of materials

Active Publication Date: 2010-08-04
无锡贝迪生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural collagen macromolecules and hydroxyapatite ceramics are components of bone extracellular matrix, which have good biocompatibility and bioactivity, and are undoubtedly ideal components for bone tissue engineering scaffold materials, but their mechanical properties are poor; Molecular materials such as poly(lactic-glycolic acid) (PLGA) have good mechanical strength, degradability and processability, which can make up for their shortcomings despite their hydrophobic surface and lack of cell recognition sites.

Method used

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  • Method for preparing bioactive poly(lactic-co-glycolic acid)/collagen/hydroxyapatite composite fiber membrane for bone repair
  • Method for preparing bioactive poly(lactic-co-glycolic acid)/collagen/hydroxyapatite composite fiber membrane for bone repair
  • Method for preparing bioactive poly(lactic-co-glycolic acid)/collagen/hydroxyapatite composite fiber membrane for bone repair

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] 1) Dissolve 1.5g of poly(lactic acid-glycolic acid) in 10mL of tetrahydrofuran / dimethylformamide mixed solvent with a volume ratio of 1 / 1, that is, the mass concentration is 15%, and the solution is added to a 20mL syringe Perform electrospinning, set the flow rate to 1.0mL / h, the voltage to 12kV, and collect the aluminum film at room temperature with a collection distance of 15cm. After 2 hours, the injection was stopped, and poly(lactic acid-glycolic acid) nanofiber membranes could be collected on the aluminum membrane;

[0036] 2) Dissolving 0.1 g of collagen in 100 mL of acetic acid solution with a volume concentration of 3%, to prepare an acetic acid solution with a mass concentration of 1 mg / mL collagen;

[0037] 3) Take 5 pieces of electrospun fiber membranes prepared in step 1), place them in a plasma discharge apparatus, set the power at 400W, and treat them for 5 minutes, 10 minutes, 15 minutes, 20 minutes, and 30 minutes respectively. Immediately immerse the...

example 2

[0044] Steps 1) to 2) are the same as steps 1) to 2) in Example 1.

[0045] Step 3) is the same as step 3) in Example 1, but the power of the plasma treatment is 50W.

[0046] Step 4) is the same as step 4) in Example 1 to obtain poly(lactic acid-glycolic acid) / collagen / hydroxyapatite composite fiber membrane.

[0047]Step 5) is the same as Step 5) in Example 1, but the ability of the fibrous membrane to induce osteogenic differentiation is evaluated with rabbit-derived bone marrow mesenchymal stem cells (MSCs), and the planting density of each well is controlled to be 6.0×10 3 / hole (i.e. 1.6×10 4 / cm 2 ). Part of the cells were cultured in DMEM medium containing 10% fetal bovine serum for up to 4 weeks, and some cells were cultured in DMEM medium containing 10% fetal bovine serum for 7 days before adding osteoinductive solution (containing 100nM dexamethasone, 10mM β- glycerol phosphate and 50 μg / mL vitamin C ascorbic acid) in DMEM medium containing 10% fetal bovine seru...

example 3

[0049] Steps 1) to 2) are the same as steps 1) to 2) in Example 1.

[0050] Step 3) is the same as step 3) in Example 1, but the poly(lactic acid-glycolic acid) fiber membrane is coated with collagen after plasma treatment for 10 minutes.

[0051] Step 4) is the same as step 4) in Example 1 to obtain poly(lactic acid-glycolic acid) / collagen / hydroxyapatite composite fiber membrane.

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Abstract

The invention discloses a method for preparing a bioactive poly (lactic-co-glycolic acid) / collagen / hydroxyapatite composite fiber membrane for bone repair. The method comprises the following steps of: treating a poly (lactic-co-glycolic acid) electrospun nanofiber membrane by using plasma, coating collagen, and immersing the poly (lactic-co-glycolic acid) electrospun nanofiber membrane into a simulated human physiologic body fluid to mineralize to obtain the poly (lactic-co-glycolic acid) / collagen / hydroxyapatite composite fiber membrane. The preparation method of the invention has the advantages of simpleness, high speed and wide material sources. By adopting the method of plasma treatment and coating, the highly-bionic nanofiber composite membrane is prepared by the steps of introducing collagen with osteocyte epimatrix into the poly (lactic-co-glycolic acid) electrospun nanofiber membrane and depositing active hydroxyapatite onto the fiber membrane, thereby obtaining. The composite fiber membrane has the advantages of favorable combination properties and convenient operation, can effectively promote the capabilities of adherence, growth and calcification osteogenesis of osteoblasts and stem cells, and is hopeful to become an ideal active bracket for bone repair.

Description

technical field [0001] The invention relates to a preparation method of a composite fiber membrane for bone repair, in particular to a preparation method of a bioactive poly(lactic acid-glycolic acid) / collagen / hydroxyapatite composite fiber membrane. Background technique [0002] Bone injury is a common disease nowadays. Many patients suffer from articular bone injuries caused by various bone and joint diseases such as rheumatism and rheumatoid or sports trauma. There are more than 10 million patients with bone injuries every year in my country, about 5 million patients who need joint replacement (the current cost of each patient is 30,000-50,000 yuan), and nearly 300,000 patients who need facial cartilage defect repair. [0003] Since the 19th century, in order to repair large-scale bone defects caused by trauma, tumor or infection, and restore limb function, bone grafting has been mainly used clinically. However, whether it is commonly used autologous bone transplantatio...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/24A61L27/18A61L27/12
Inventor 高长有劳丽红朱旸邱媛张裕英
Owner 无锡贝迪生物工程股份有限公司
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