Ceramide-containing tea seed extract and preparation method thereof

Abstract

The invention discloses ceramide-containing tea seed extract and a preparation method thereof. The preparation method comprises the following steps of: crushing raw materials, extracting by using water or low-carbon alcohol, and recycling an extraction solvent from the obtained extracting solution so as to obtain extract, wherein the raw materials are seeds of theaceae camellia plants, namely camellia oleifera abel, camellia or common camellia, or residues of the seeds of the plants subjected to oil component extraction; adding alkaline solution into the extract, and hydrolyzing at the temperature of between 10 and 80 DEG C for 0.5 to 20 hours; and separating the obtained hydrolyzate by using a macroporous resin column, performing chromatography through a silica gel column or a sephadex column, eluting by using 40 to 60 volume percent aqueous solution of methanol or ethanol, performing thin layer chromatography, collecting fractions, mixing the fractions, and drying to obtain the ceramide-containing tea seed extract. The raw materials are readily available and the method is environmentally-friendly; the preparation process is simple; the product quality is stable; the production cost is low; and the content of ceramide in the extract is high.

Description

technical field

[0001] The invention relates to the extraction of active ingredients in plants, in particular to tea seed extract containing ceramide and a preparation method thereof. Background technique

[0002] Ceramide is composed of long-chain sphingosine covalently bonded to fatty acid through amide bond. Due to the difference in the carbon chain length of sphingosine in the ceramide molecule, the difference in the degree of unsaturation and hydroxylation, and the length of the fatty acid connected to the sphingosine through an amide bond, the number and position of unsaturated bonds and hydroxyl groups Different substances show great differences, so to be precise, ceramide is not a kind of substance, but a kind of substance with similar structure. Kristi JRobSon et al. have done detailed research on ceramides in the stratum corneum of the skin, and divided ceramides into seven types according to their structures. Ceramides are generally divided into Ceramide(I)-Cera...

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