Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving

Abstract

The invention discloses a bacillus subtilis HL-1 and an application thereof in the respect of soil phosphate dissolving. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on Aug 24th, 2011, with a collection number of CGMCC No.5175. The 16SrDNA nucleotide sequence of the strain is represented by SEQ ID NO:1. With added diatomite and guava slag adsorbing agent materials, a fermentation broth of the bacillus subtilis HL-1 can be prepared into microbial manure. With the strain provided by the invention, phosphate availability and phosphate fertilizer utilization rate of the soil can be improved, plant growth can be promoted, and soil microbe diversity can be improved. The strain has important significance in reducing chemical fertilizer application, modifying soil micro-ecology, and promoting soil sustainable utilization. The strain has wide application prospect in production practice.

Description

technical field [0001] The invention relates to a microorganism and its application, in particular to a Bacillus subtilis HL-1 and its application in decomposing soil insoluble phosphorus, improving soil micro-ecological structure, promoting plant growth and the like. Background technique [0002] Phosphorus is one of the nutrient elements necessary for plant growth and development, and has important effects on nitrogen fixation, photosynthesis, energy transfer, signal transduction, biomacromolecule synthesis and respiration. There are 5.7 billion hm in the world 2 The soil is deficient in phosphorus, and the phosphorus concentration in most soils is from 0.1 to 10 μmol / L. Under ideal conditions, the phosphorus concentration required for the growth of gramineous plants is 1-5 μmol / L, and the phosphorus concentration required for high phosphorus-demanding plants such as tomatoes and peas is 1-5 μmol / L. 5~60μmol / L. When phosphorus does not reach the ideal state, the plant wi...

AI Technical Summary

Problems solved by technology

The use of traditional chemical fertilizers can alleviate the lack of phosphorus in the soil to a certain extent, but as the main varieties of phosphate fertilizers in China, such as superphosphate and ammonium phosphate, no matter in the calcareous soil in the north or the acidic soil in the south, due to the strong phosphorus fixation effect of the soil , the quarterly utilization rate is less than 20%, resulting in waste of resources

However, it has not been reported that guava residue, a renewable resource, is used as a bio-fertilizer adsorbent material to replace the commonly used non-renewable resource to produce a bacterial agent with high phosphorus-decomposing effect.

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