Preparation method of silk fibroin nanosphere

Abstract

The invention discloses a preparation method of silk fibroin nanospheres, which comprises the following steps: dispersing a silk fibroin solution into nanometer liquid drops by an inverse emulsion method, adding polyols under ultrasonic oscillation to promote silk fibroin in the nanometer liquid drops to form a structure insoluble in water, adding high water-absorbing resins to dry and cure the silk fibroin in a microemulsion state so as to obtain silk fibroin nanospheres which are insoluble in water. The silk fibroin nanospheres have good biocompatibility, are nontoxic and harmless for human body, and have no immunogenicity; the preparation process adopts mild technology with a normal temperature, a normal pressure, and no chemical cross-linking agents; therefore, the product can be used as a carrier with biologically-active substances to load enzymes, nucleic acids, polypeptides, protein drugs, and the like; the obtained silk fibroin spheres have small particle sizes, good dispersibility, do not adhere, and can be prepared into various cosmetics, skin care products, sunscreen creams and the like; the silk fibroin spheres have small particles which can run freely in blood, thus can be used as injections and have wide application in fields of disease diagnosis and treatment.

Description

technical field

[0001] The invention relates to a method for preparing organic polymer nanospheres, in particular to a method for preparing nanoparticles by using silk fibroin as a raw material, and belongs to the technical field of biomedicine. Background technique

[0002] In recent years, with the development of biotechnology and genetic engineering, more and more biologically active substances such as proteins and enzymes are expected to be used in the treatment of diseases. However, since protein and polypeptide drugs are easily degraded in the intestinal tract after oral administration, the circulation time in the body is short and the biological half-life is short after intravenous injection, which greatly restricts their application. To solve these problems, various protein drug dosage forms have emerged, including hydrogels, nanospheres, microspheres, liposomes of lipoplexes, solid lipid nanoparticles, water-in-oil emulsions, and the like. Microspheres made of biod...

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