Salmonella culture medium containing crystal violet and production process of the culture medium

Abstract

The invention discloses a salmonella culture medium that contains crystal violet and a production process of the culture medium. The culture medium is prepared from the following raw materials by weight: 5-15 parts of peptone, 5-15 parts of lactose, 0.1-1 part of L-cysteine, 1-9 parts of beef powders, 1-9 parts of sodium chloride, 1-9 parts of sodium citrate, 5-15 parts of cholate, 1-9 parts of sodium thiosulfate, 0.001-0.01 parts of crystal violet, 0.5-1.5 parts of ferric ammonium citrate, 0.050-0.070 parts of bromothymol blue and 11-18 parts of agar. According to the invention, the operation is simple and convenient, the necessary types of culture media for detection can be reduced, the detection time is shortened from 4-7d in a method in prior art to 2d, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to a culture medium for Salmonella crystalline purple and a production process thereof. [0002] Background technique [0003] Culture medium refers to the artificially prepared nutrient matrix suitable for the growth and reproduction of bacteria, which can be used for the cultivation, separation, research and preservation of microorganisms. , Agricultural microbial tissue culture, industrial microbial inspection, such as food inspection, pharmaceutical biological inspection, cosmetic inspection, drinking water inspection, disposable hygiene product inspection and many other industries to test bacteria, have a wide range of applications and progress, and are closely related to human life. [0004] Salmonellosis is one of the zoonotic diseases of great significance in public health, including those bacteria that cause food poisoning, gastroenteritis, typhoid fever and paratyphoid fever. The traditional culture and detection time t...

AI Technical Summary

Problems solved by technology

However, due to the selectivity and specificity of the traditional culture medium for detecting Salmonella at present cannot reach satisfactory results, (the formula of the traditional culture medium has the following several: 1. buffered peptone water enrichment, formula: 10 grams of peptone, sodium chloride 5 grams, 9 grams of disodium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate; 1g of bile salt, 7g of calcium carbonate, 0.01g of brilliant green, 20ml of 0.1mol / L iodine solution; and GN enrichment solution, formula: 20g of tryptone, 1g of glucose, 2g of mannitol, citric acid 5 grams of sodium, 0.5 grams of sodium deoxycholate, 4 grams of dipotassium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate, and 5 grams of sodium chloride; 3, transfer to HE agar plate for separation, formula: 12 grams of peptone, 3 grams of beef extract gram, lactose 12 grams, sucrose 12 grams, salicin 2 grams, bile salt 20 grams, sodium chloride 5 grams, agar 15 grams, bromothymol blue 0.064 grams, acid fuchsin 0.1 grams, sodium thiosulfate 6.8 grams, iron citrate press 0.8 grams, sodium deoxycholate 2 grams

) The detection time of Salmonella is relatively long and the resolution is low, which can only be judged by experience, and it is quite difficult for beginners to judge the results