Salmonella culture medium containing crystal violet and production process of the culture medium

A Salmonella culture medium and crystal violet technology, applied in the direction of microbial determination/testing, biochemical equipment and methods, and resistance to vector-borne diseases, can solve the problem of unsatisfactory results of selectivity and specificity, initial It is quite difficult to judge the results of scholars, and the detection time of Salmonella is long, etc., so as to achieve the effect of reducing the types of culture medium, improving the detection sensitivity and easy operation

Inactive Publication Date: 2012-07-25
绍兴天恒生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the selectivity and specificity of the traditional culture medium for detecting Salmonella at present cannot reach satisfactory results, (the formula of the traditional culture medium has the following several: 1. buffered peptone water enrichment, formula: 10 grams of peptone, sodium chloride 5 grams, 9 grams of disodium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate; 1g of bile salt, 7g of calcium carbonate, 0.01g of brilliant green, 20ml of 0.1mol / L iodine solution; and GN enrichment solution, formula: 20g of tryptone, 1g of glucose, 2g of mannitol, citric acid 5 grams of sodium, 0.5 grams of sodium deoxycholate, 4 grams of dipotassium hydrogen phosphate, 1.5 grams of potassium dihydrogen phosphate, and 5 grams of sodium chloride; 3, transfer to HE agar plate for separation, formula: 12 grams of peptone, 3 grams of beef extract gram, lactose 12 grams, sucrose 12 grams, salicin 2 grams, bile salt 20 grams, sodium chloride 5 grams, agar 15 grams, bromothymol blue 0.064 grams, acid fuchsin 0.1 grams, sodium thiosulfate 6.8 grams, iron citrate press 0.8 grams, sodium deoxycholate 2 grams
) The detection time of Salmonella is relatively long and the resolution is low, which can only be judged by experience, and it is quite difficult for beginners to judge the results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0038] Peptone 10g; lactose 10g; L-cysteine ​​0.5g; beef powder 5g; sodium chloride 5g; sodium citrate 5g; bile salt 10g; sodium thiosulfate 5g; crystal violet 0.005g; Ferric ammonium citrate 1 g; bromothymol blue 0.064 g; agar 12 g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri dish, 15-...

Embodiment 2

[0041] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0042]Peptone 5 g; lactose 5 g; L-cysteine ​​0.1 g; beef powder 1 g; sodium chloride 1 g; sodium citrate 1 g; bile salt 5 g; sodium thiosulfate 1 g; crystal violet 0.001 g ; ferric ammonium citrate 0.5 g; bromothymol blue 0.050 g; agar 11 g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri d...

Embodiment 3

[0046] A kind of Salmonella crystalline purple culture medium of the present embodiment, the weight portion of described raw material is:

[0047] Peptone 15 g; lactose 15 g; L-cysteine ​​1 g; beef powder 9 g; sodium chloride 9 g; sodium citrate 9 g; bile salt 15 g; sodium thiosulfate 9 g; crystal violet 0.01 g ; ferric ammonium citrate 1.5 g; bromothymol blue 0.070 g; agar 18 g. The production process of the culture medium in this example includes the following steps: First, weigh all the raw materials except agar according to the above ratio, mix them evenly, put them in distilled water, heat them with an electric furnace until they dissolve, and keep stirring; after they are completely dissolved, cool them down naturally To 25-30°C, use sodium carbonate to adjust the pH value to 7.2-7.4, then add the agar of the above ratio for three times of boiling sterilization, after sterilization, the pH value reaches 7.2-7.4; when it is cooled to about 55°C, pour 90mm of clean Petri ...

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PUM

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Abstract

The invention discloses a salmonella culture medium that contains crystal violet and a production process of the culture medium. The culture medium is prepared from the following raw materials by weight: 5-15 parts of peptone, 5-15 parts of lactose, 0.1-1 part of L-cysteine, 1-9 parts of beef powders, 1-9 parts of sodium chloride, 1-9 parts of sodium citrate, 5-15 parts of cholate, 1-9 parts of sodium thiosulfate, 0.001-0.01 parts of crystal violet, 0.5-1.5 parts of ferric ammonium citrate, 0.050-0.070 parts of bromothymol blue and 11-18 parts of agar. According to the invention, the operation is simple and convenient, the necessary types of culture media for detection can be reduced, the detection time is shortened from 4-7d in a method in prior art to 2d, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to a culture medium for Salmonella crystalline purple and a production process thereof. [0002] Background technique [0003] Culture medium refers to the artificially prepared nutrient matrix suitable for the growth and reproduction of bacteria, which can be used for the cultivation, separation, research and preservation of microorganisms. , Agricultural microbial tissue culture, industrial microbial inspection, such as food inspection, pharmaceutical biological inspection, cosmetic inspection, drinking water inspection, disposable hygiene product inspection and many other industries to test bacteria, have a wide range of applications and progress, and are closely related to human life. [0004] Salmonellosis is one of the zoonotic diseases of great significance in public health, including those bacteria that cause food poisoning, gastroenteritis, typhoid fever and paratyphoid fever. The traditional culture and detection time t...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
CPCY02A50/30
Inventor 朱祝鸿
Owner 绍兴天恒生物科技有限公司
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