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Method for promoting cellulose anaerobic degradation-based methane production

A technology of cellulose and methane production, applied in the direction of biological sludge treatment, waste fuel, fermentation, etc., can solve the problem that the conversion rate of straw cellulose raw materials has not been improved, the conversion rate of straw cellulose cannot be improved, and the types and conversion rates of raw materials are not good. Pay attention to other issues, achieve good stability and practical value, improve degradation ability and methane production ability, and achieve low cost effects

Inactive Publication Date: 2013-11-27
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

Microbial preparations mainly provide specific microorganisms to improve biogas fermentation conditions by increasing the number of microorganisms, but the conversion rate of raw materials still needs to be improved; biological enzyme preparations mainly include cellulase, pectinase and laccase, etc., but the cost is relatively high The biological enzyme cofactors related to biogas fermentation are mainly heavy metals such as cobalt and nickel. Growth and use require high technology. At the same time, heavy metals will remain in the environment (soil and water quality), destroying the ecological environment
[0004] Chinese patent CN 101016558A discloses a method for promoting biogas fermentation at low temperature. The invention proposes that bamboo acetic acid can accelerate the production of aquaculture waste at a relatively low temperature (10-15°C). , the biogas production rate of the mixture of crop straw and domestic wastewater, but the conversion rate of straw cellulose raw material has not been improved
Chinese patent CN102732566 A discloses a biogas accelerator in cold regions and its preparation method. The main components of the accelerator are iron, cobalt, nickel, organic substances, adsorbents and fillers. The technical solution of this invention is to improve biogas at low temperature Yield, do not pay attention to the type of raw material and conversion rate; and if iron, cobalt, nickel can improve the metabolic activity of methanogens, but can not improve the conversion rate of straw cellulose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare cellulose-containing culture solution, divide 150ml into 250ml saline bottles, add the mixed solution of calcium pantothenate and tetrahydrofolate to the medium before inoculation, the volume ratio of the mixed solution of calcium pantothenate and tetrahydrofolate to the medium is 1 :500, the inoculum size is 5% (V / V), sealed, and cultured statically at 40°C. The formula of cellulose-containing culture solution is as follows: NaCl 3‰, cellulose (straw) 5‰, urea 1‰, peptone 1‰, yeast powder 0.5‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 The ratio of the mass of , NaCl, cellulose, urea, peptone, and yeast powder to the total volume of the medium. The mass ratio of calcium pantothenate and tetrahydrofolate is 5:2. The inoculum is a complex cellulose-degrading and methanogenic bacteria obtained in the laboratory. The difference between the control group and the experimental group is that the mixed solution of calcium pantothenate ...

Embodiment 2

[0030] Prepare cellulose-containing culture solution, divide 150ml into 250ml saline bottles, add the mixed solution of calcium pantothenate and tetrahydrofolate to the medium before inoculation, the volume ratio of the mixed solution of calcium pantothenate and tetrahydrofolate to the medium is 1 :500, the inoculum size is 5% (V / V), sealed, and cultured statically at 40°C. The formula of cellulose-containing culture solution is as follows: NaCl 3‰, cellulose (straw) 5‰, urea 1‰, peptone 1‰, yeast powder 0.5‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 The ratio of the mass of , NaCl, cellulose, urea, peptone, and yeast powder to the total volume of the medium. The mass ratio of calcium pantothenate and tetrahydrofolate is 2:1. The inoculum is a complex cellulose-degrading and methanogenic bacteria obtained in the laboratory. The difference between the control group and the experimental group is that the mixed solution of calcium pantothenate ...

Embodiment 3

[0034] Prepare cellulose-containing culture solution, divide 150ml into 250ml saline bottles, add the mixed solution of calcium pantothenate and tetrahydrofolate to the medium before inoculation, the volume ratio of the mixed solution of calcium pantothenate and tetrahydrofolate to the medium is 1 : 1000, inoculum size 5% (V / V), sealed, 40 ℃ static culture. The formula of cellulose-containing culture solution is as follows: NaCl 3‰, cellulose (straw) 5‰, urea 1‰, peptone 1‰, yeast powder 0.5‰, CaCO 3 2‰, with water as the solvent, the above content refers to CaCO 3 The ratio of the mass of , NaCl, cellulose, urea, peptone, and yeast powder to the total volume of the medium. The mass ratio of calcium pantothenate and tetrahydrofolate is 2:1. The inoculum is a complex cellulose-degrading and methanogenic bacteria obtained in the laboratory. The difference between the control group and the experimental group is that the mixed solution of calcium pantothenate and tetrahydrofol...

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Abstract

The invention provides a method for promoting cellulose anaerobic degradation-based methane production. The method comprises the following steps of mixing calcium pantothenate and tetrahydrofolic acid according to a certain ratio, adding the mixed solution into a biogas fermentation system with fermentation raw materials, and carrying out biogas fermentation production, wherein the main fermentation raw material is straw cellulose and a volume ratio of the mixed solution of calcium pantothenate and tetrahydrofolic acid to the fermentation system is 1: 500-2000. The method is simple and effective, has a low cost and a wide application range, avoids crop straws retting re-treatment and improves cellulose degradation efficiency and biogas output.

Description

technical field [0001] The invention belongs to the technical field of development and utilization of agricultural waste biomass energy, and in particular relates to a method for promoting the anaerobic degradation of cellulose to produce methane. Background technique [0002] Cellulose is the most abundant renewable resource in the world and one of the important sources of raw materials for renewable energy production such as biogas. However, due to the dense molecular structure of straw cellulose and the protection of hemicellulose and lignin, straw cellulose is not easy to be degraded by microorganisms, the hydrolysis speed is slow, and the conversion rate is low, which is a technical problem in the development and utilization of renewable energy. [0003] In the previous technical research, the improvement of the utilization rate of straw cellulose mainly relied on the pretreatment technology of raw materials. Biogas fermentation and other issues, straw cellulose utiliz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C02F11/04
CPCY02E50/343Y02E50/30
Inventor 马诗淳邓宇李强尹小波
Owner BIOGAS SCI RES INST MIN OF AGRI
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