Preparation of superoxide anion sensor based on noble metal alloy/carbon composite material

A technology of superoxide anion and carbon composite material, applied in the field of sensors, can solve the problems of lack of solid enzyme interface, narrow linear range, low sensitivity, etc., and achieve the effects of good stability and repeatability, wide linear range and high sensitivity
CN104034786AActive Publication Date: 2014-09-10EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2014-09-10

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Abstract

The invention relates to preparation of a superoxide anion sensor based on a noble metal alloy / carbon composite material. The preparation specifically comprises the steps of loading the noble metal alloy material on the surface of a carbon material serving as a carrier through a chemical reaction so as to form a noble metal alloy / carbon material compound with high catalysis performance; and modifying the noble metal alloy / carbon material compound on the surface of an electrode to form an electrochemical interface with high biocompatibility, and then fixing superoxide dismutase on the surface of the electrode, thus obtaining the superoxide anion sensor. According to the preparation, the noble metal alloy is loaded on the carbon material, and then the SOD (superoxide dismutase) is fixed; the carbon material supplies a friendly fixing environment to superoxide dismutase; due to the noble metal alloy, the catalysis performance of the sensor is improved, and electronic transmission between superoxide dismutase and the electrode is improved; the constructed biosensor has the characteristics of wide linear range, high sensitivity, short response time, high stability and high repetitiveness, and can accurately and conveniently perform qualitative and quantitative detection on O2<-1>.
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Description

【Technical field】

[0001] The invention relates to the technical field of sensors, in particular to the preparation of a superoxide anion sensor based on a precious metal alloy / carbon composite material. 【Background technique】

[0002] Reactive oxygen species (ROS) play important physiological functions in the processes of biological aging, disease, immune metabolism and cell signal transduction. Among them, the superoxide anion radical (O 2 ·- ) is an important ROS, which can cause damage to biomolecules, but at the same time has a signaling pathway role in the process of cell signal transduction. Because free radicals are relatively active and have a short lifespan, it is still a difficult point to establish an efficient and reliable qualitative and quantitative detection method.

[0003] Electron spin resonance, spectrophotometry, chemiluminescence, chromatography and electrochemical methods are commonly used in O 2 ·- detection. Compared with other detection methods...

Claims

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