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Preparation of superoxide anion sensor based on noble metal alloy/carbon composite material

A technology of superoxide anion and carbon composite material, applied in the field of sensors, can solve the problems of lack of solid enzyme interface, narrow linear range, low sensitivity, etc., and achieve the effects of good stability and repeatability, wide linear range and high sensitivity

Active Publication Date: 2014-09-10
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the lack of a friendly immobilized enzyme interface, the currently prepared SOD sensors have low sensitivity and narrow linear range. Researchers have tried to use carbon materials and alloy materials as enzyme carriers to prolong the service life of the enzyme electrode and improve the sensitivity of the sensor.
However, as far as we know, there are no reports on the use of noble metal alloy / carbon material composites to construct superoxide anion radical sensors at home and abroad.

Method used

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  • Preparation of superoxide anion sensor based on noble metal alloy/carbon composite material
  • Preparation of superoxide anion sensor based on noble metal alloy/carbon composite material
  • Preparation of superoxide anion sensor based on noble metal alloy/carbon composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Preparation of Pd-Pt / MWCNTs

[0054] (1) Functionalization of carbon nanotubes

[0055] Dissolve 1g MWCNTs into 50ml, 30%H 2 o 2 , sonicated for 4h to disperse the MWCNTs uniformly in the solution. Then filter with a 450nm membrane, wash with ultrapure water three times, and vacuum dry at 45°C for 12h. The MWCNTs treated above were added into the mixed solution of nitric acid and sulfuric acid (HNO 3 :H 2 SO 4 =1:3), 80 after 30min of ultrasound. The acidification reaction was carried out under reflux for 8 h at ℃ to introduce oxygen-containing groups such as carboxyl and hydroxyl groups on the surface of MWCNTs to increase the water solubility of MWCNTs. The product was washed with ultrapure water until neutral. Dry in a vacuum oven at 45°C before use.

[0056] (2) Preparation of Pd-Pt / MWCNTs

[0057] A: Take 10 mg of acidified MWCNTs in a small beaker, add 10 ml (water: ethylene glycol = 1: 1) mixed solution, and sonicate for 30 minutes to disperse MWCNTs ev...

Embodiment 2

[0062] Preparation of SOD / Pt-Pd / MWCNTs / SPGE Electrode

[0063] (1) Preparation of screen-printed gold film electrode (SPGE)

[0064] Strictly speaking, the screen-printed gold electrode is not simply a screen-printed electrode, because its preparation not only uses screen printing technology but also combines vacuum evaporation technology. Design drawings include (1) conductive silver layer, (2) Ag / AgCl reference layer, (3) carbon counter electrode layer, (4) insulating layer and (5) gold mask. The specific preparation process is as follows: The specific preparation steps are: (1) Print conductive silver paste on the PET substrate to form electrode strips, and heat-cure in an oven at 120° C. for 40 minutes. (2) Print the Ag / AgCl reference layer and heat cure it at 80°C for 10min. The final electrode area is 35mm×12mm (length×width), and the diameter of the working end is 3mm. (3) Print conductive carbon paste to form a carbon counter electrode layer, and heat-cure at 71°C fo...

Embodiment 3

[0071] Preparation and functionalization of graphene

[0072] Use the Hummers method to prepare graphene oxide and functionalize its surface. The specific method is as follows: First, add 20g NaCl to 1g graphite powder, shake for 10-15min, then filter and wash off NaCl, and dry in an oven at 70°C for 30min; then transfer Add 23mL concentrated sulfuric acid to a 250mL round bottom flask, stir at room temperature for 24h; then heat to 40°C in a water bath, add 100mg NaNO 3 , stirred for 5min to dissolve, slowly added 500mg KMnO 4 , add 3mL of water after 5min, add 3mL of water after 5min, add 40mL of water after 5min, remove from the water bath after 15min, add 140mL of water, add 10mL of 30% H 2 o 2 Neutralize unreacted KMnO 4 ; Finally, the solution obtained is subjected to centrifugation (5000rpm, 15min), and the lower layer solid matter is centrifugally washed with 100mL ultrapure water and 100mL5% HCl after centrifugation, and then washed with a large amount of water to ...

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Abstract

The invention relates to preparation of a superoxide anion sensor based on a noble metal alloy / carbon composite material. The preparation specifically comprises the steps of loading the noble metal alloy material on the surface of a carbon material serving as a carrier through a chemical reaction so as to form a noble metal alloy / carbon material compound with high catalysis performance; and modifying the noble metal alloy / carbon material compound on the surface of an electrode to form an electrochemical interface with high biocompatibility, and then fixing superoxide dismutase on the surface of the electrode, thus obtaining the superoxide anion sensor. According to the preparation, the noble metal alloy is loaded on the carbon material, and then the SOD (superoxide dismutase) is fixed; the carbon material supplies a friendly fixing environment to superoxide dismutase; due to the noble metal alloy, the catalysis performance of the sensor is improved, and electronic transmission between superoxide dismutase and the electrode is improved; the constructed biosensor has the characteristics of wide linear range, high sensitivity, short response time, high stability and high repetitiveness, and can accurately and conveniently perform qualitative and quantitative detection on O2<-1>.

Description

【Technical field】 [0001] The invention relates to the technical field of sensors, in particular to the preparation of a superoxide anion sensor based on a precious metal alloy / carbon composite material. 【Background technique】 [0002] Reactive oxygen species (ROS) play important physiological functions in the processes of biological aging, disease, immune metabolism and cell signal transduction. Among them, the superoxide anion radical (O 2 ·- ) is an important ROS, which can cause damage to biomolecules, but at the same time has a signaling pathway role in the process of cell signal transduction. Because free radicals are relatively active and have a short lifespan, it is still a difficult point to establish an efficient and reliable qualitative and quantitative detection method. [0003] Electron spin resonance, spectrophotometry, chemiluminescence, chromatography and electrochemical methods are commonly used in O 2 ·- detection. Compared with other detection methods...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
Inventor 蓝闽波朱翔赵红莉袁慧慧唐杰刘婷婷牛湘衡胡杨了
Owner EAST CHINA UNIV OF SCI & TECH
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