Cellulosimicrobium cellulans strain and application thereof

Abstract

The invention relates to a cellulosimicrobium cellulans strain and application thereof. The strain is a Cellulosimicrobium cellulans strain F16 of which the preservation number is CCTCC M2013201 and the preservation date is in may, 2013; the Cellulosimicrobium cellulans strain F16 or a hydrolase generated by virtue of the fermentation of the Cellulosimicrobium cellulans strain F16 are capable of hydrolyzing a compound which contains a xyloside bond and a glucoside bond to obtain a product, namely a corresponding aglycone or an intermediate compound generated in the preparation process of the corresponding aglycone.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a fibrosis cellulosic microbacteria strain and application thereof. Background technique [0002] Many natural active compounds often exist in nature in the form of glycosides, and the active compounds can be released after the sugar group is removed. Such as the anticancer drug paclitaxel, which is mainly found in the bark of the yew plant. In the branches and leaves of yew, there are a large number of xylosylated paclitaxel precursors or their analogs of the same mother nucleus (called taxane compounds), such as 7-xylose paclitaxel and 7-xylose-10 Deacetylpaclitaxel (10DAXT), its accumulation in the branches and leaves of Taxus chinensis, Yunnan and Southern Taxus can be more than 10 times higher than that of paclitaxel, and the 7-xylosyl group can be hydrolyzed and then converted into paclitaxel by one step of acetylation . [0003] Another typical natural pr...

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Problems solved by technology

There have been many reports on enzymatic hydrolysis of glycoside compounds. For example, Korean researchers have announced the use of Aspergillus aculus to hydrolyze ginsenosides in red ginseng to increase the content of ginsenoside aglycone (J.Korean Soc.Appl.Biol.Chem .53(5), 553-558(2010)), Kohada reported that G-Rb1, Rb2, Rb3, Rc and Rd were used as raw materials, and the sugar on the C-20 position was removed by acetic acid hydrolysis, and then crude hesperidin 20(S)-PPD (JPO58-131999) is obtained by enzymatic hydrolysis to remove the sugar at the 3-position. Chinese patent CN03101549.2 discloses a method for preparing ginseng composition through biotransformation of lactic acid bacteria or intestinal bacteria. Chinese patent CN102703329 A strain capable of efficiently transforming saponins in turmeric to produce diosgenin was disclosed. Hanson RL (1997) used bacteria Moraxella sp., Bacillus macerans, Bacillus circulans and Micrococcus sp. to convert C-7 xylose taxane into C- 7-hydroxytaxane, Chinese patent CN102296053 discloses that a β-xylosidase from fungi also has the ability to hydrolyze C-7 xylose taxane, and realize its intracellular expression in yeast, etc., but often exists In the case of bacterial transformation, enzyme production and low transformation efficiency, especially for some compounds with poor water solubility and large steric hindrance, such as 10DAXT, ordinary glycosidases are often difficult to hydrolyze

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