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Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof

A technology for marine aquatic products and purifying agents, applied in biochemical equipment and methods, chemical instruments and methods, fixed on or in inorganic carriers, etc., can solve problems such as unsuitability for marine aquaculture, rare bacillus, and achieve high-efficiency removal Remove nitrous nitrogen, reduce activation time, and overcome the effects of frequent epidemics

Active Publication Date: 2015-07-22
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these bacillus are freshwater strains, which are not suitable for mariculture. There are few studies on bacillus and their microbial preparations that are specially used for mariculture and have high-efficiency purification of seawater.

Method used

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  • Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
  • Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
  • Carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening, isolation and identification of embodiment 1 bacterial strain YB-01 and YB-02

[0042] 1. Preparation of culture medium:

[0043] Ammonia nitrogen degrading bacteria enrichment screening medium: (NH4) 2 SO 4 2g, NaH 2 PO 4 0.25g, MnSO 4 4H 2 O0.01g, K 2 HPO 4 0.75g, MgSO 4 ·7H 2 O 0.03g, CaCO 3 3g, aged sea water 1L, pH 7.5-8.0.

[0044] Solid medium for separation and purification of ammonia nitrogen degrading bacteria: (NH4) 2 SO 4 0.5g, NaH 2 PO 4 0.25g, MnSO 4 4H 2 O 0.01g, K 2 HPO 4 0.75g, MgSO 4 ·7H 2 O 0.03g, CaCO 3 2g, aged sea water 1 L, agar powder 12g, pH 7.5-8.0.

[0045] Nitrite nitrogen degrading bacteria enrichment screening liquid medium: NaNO 2 1g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g, FeSO 4 ·7H 2 O 0.4g, aged sea water 1L, pH 7.2-7.5.

[0046] Solid medium for isolation and purification of nitrite nitrogen degrading bacteria: NaNO 2 0.5g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g...

Embodiment 2

[0058] Physiological and biochemical characteristics of embodiment 2 bacterial strains YB-01 and YB-02

[0059] YB-01 and YB-02 were detected according to the physiological and biochemical detection methods of the "Bergery Bacteria Identification Handbook" (Eighth Edition), and the detection indicators included sugar fermentation, alcohol fermentation, starch hydrolysis, lipase, methyl acetylphthalein, Catalase, casein hydrolysis, protein hydrolysis, citrate utilization, tyrosine hydrolysis, phenylalanine deamination and acid production, etc.

[0060] Table 1. Physiological and biochemical characteristics of YB-01 and YB-02 strains

[0061]

[0062] Note: In Table 1, means positive, and means negative.

Embodiment 3

[0063] The performance detection of embodiment 3 bacterial strain YB-01 and YB-02

[0064] (1) Detection of ammonia nitrogen and nitrite nitrogen removal rate: the national standard phenol hypochlorite colorimetric method and naphthalene ethylenediamine hydrochloride diazo azo colorimetric method (GB_17378.4-2007) were used to detect ammonia nitrogen and nitrite nitrogen respectively state nitrogen concentration. Under simulated seawater farming conditions (mainly salinity 32‰, pH 7.5, temperature 24-26, dissolved oxygen ≧5mg / L), the initial concentration of ammonia nitrogen of YB-01 or YB-02 is 5, 10, 25 and 50mg respectively. The 24-hour ammonia nitrogen removal rate of / L simulated solution; and the 24-hour nitrite nitrogen removal rate of the two strains to the simulated solution with initial concentration of nitrite nitrogen of 0.5, 2, 5 and 10mg / L, each concentration gradient Set up 3 repetitions. The preparation method of YB-01 or YB-02 used for detection is: YB-01 or...

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Abstract

The invention discloses a carrier-immobilized bacterial inhibition type water purifier for marine aquaculture and a preparation method thereof. The carrier-immobilized bacterial inhibition type water purifier is prepared by adsorbing and curing a mixed fermentation solution containing bacillus subtilis YB-01 CGMCC-No.9756 and brevibacillus laterosporus YB-02 CGMCC-No.9757. The strain of the water purifier is bacillus separated from a seawater source and has an excellent water purification effect and bacterial inhibition effect; kieselguhr and corncob powder are used as viable bacteria carriers, so that the water purifier is high in environmental tolerance, highly controllable in floatability, suspension property and sinking and floating properties and remarkable in three-dimensional purification effect on aquaculture water. By virtue of the water purifier, the harmful materials such as ammonia nitrogen, nitrite and hydrogen sulfide in the marine aquaculture water can be quickly degraded; the heavy metal ions in bottom mud can be efficiently adsorbed; deterioration, acidification and smelly bottom mud of the water can be avoided; the growth of main pathogenic bacteria in the marine aquaculture can be inhibited; diseases can be effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microbes, and in particular relates to a carrier-immobilized water quality purifier for the culture of sea cucumbers, turbot and red-fin puffers and other marine products with strong adaptability to the survival and reproduction of bacterial flora and its immobilized water quality. Preparation methods and applications. Background technique [0002] Seawater is the basic environmental condition for the survival of seawater animals. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects their growth status and the economic benefits of farmers. With the improvement of living standards, people's demand for aquatic products is increasing day by day. When the catch cannot meet the market demand, high-density intensive aquaculture has developed rapidly. However, the degree of pollution of the water body in this aquaculture model...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/02C02F3/34C12R1/125C12R1/01
Inventor 徐永平车鉴李淑英李晓宇王丽丽王熙涛张建成阳桂菊
Owner DALIAN UNIV OF TECH
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