Preparation method of bone tissue engineering porous ceramics scaffold

A technology of bone tissue engineering and porous ceramics, applied in tissue regeneration, pharmaceutical formulations, coatings, etc., can solve the problems of limitations, inability to embed and release drugs, and inability to ensure the distribution of microspheres, and achieve the effect of promoting tissue growth

Inactive Publication Date: 2016-04-06
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a preparation method of bone tissue engineering porous ceramic support, which can effectively solve the problem of coating drug-loaded shell on the surface of bone tissue engineering porous ceramic support Uniform distribution of polysaccharide microspheres

Method used

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  • Preparation method of bone tissue engineering porous ceramics scaffold
  • Preparation method of bone tissue engineering porous ceramics scaffold
  • Preparation method of bone tissue engineering porous ceramics scaffold

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A, the preparation of drug-loaded microspheres: the chitosan of 0.25g is dissolved in the glacial acetic acid solution of 10ml2%, then add 0.125g salvianolic acid B and mix evenly, then 10ml salvianolic acid B / chitosan mixed solution Slowly add dropwise to the kerosene system in 100ml oil phase containing 2ml Span 80 and stir for 2h, then add 1ml6% glutaraldehyde and stir for 2h, then centrifuge to collect the prepared drug-loaded chitosan microspheres, respectively wash with isopropanol , after washing with ethanol and deionized water, the drug-loaded microspheres were prepared by freeze-drying after being frozen at minus -10°C.

[0024] B. Preparation of ceramic support: add 0.75g of chitin into dimethylacetamide solvent containing 5% lithium chloride, and then add 25g of inorganic powder to prepare inorganic powder / chitin slurry. Then the sugar ball particles are deposited in the bracket mold, and then heat treatment is carried out at a temperature of 60°C for 10 min...

example 2

[0028] A, the preparation of drug-loaded microspheres: the chitosan of 0.25g is dissolved in the glacial acetic acid solution of 10ml2%, then adds 0.031g salvianolic acid B and mixes evenly, then 10ml salvianolic acid B / chitosan mixed solution Slowly add dropwise to the kerosene system in 100ml oil phase containing 2ml Span 80 and stir for 2h, then add 1ml25% glutaraldehyde and stir for 2h, then centrifuge to collect the prepared drug-loaded chitosan microspheres, respectively wash with isopropanol , after washing with ethanol and deionized water, the drug-loaded microspheres were prepared by freeze-drying after being frozen at minus -10°C.

[0029] C. Preparation of coated alginic acid stent: Dissolve 0.5g of alginic acid in 100ml of deionized water to prepare the alginic acid solution, immerse the porous stent in step B in the above-mentioned alginic acid solution for 10min and take it out at a speed of 2000r / min under centrifugation. Repeat 4 times, take out after immersin...

example 3

[0033] A. Preparation of drug-loaded microspheres: Dissolve 0.5g of chitosan in 10ml of 2% glacial acetic acid solution, then add 0.063g of bone morphogenetic protein and mix evenly, then mix 10ml of bone morphogenetic protein / chitosan mixture Slowly added dropwise to the 100ml liquid paraffin system containing 2ml Span 80 and stirred for 2h, then added 25ml5% sodium tripolyphosphate and stirred for 3h, then centrifuged to collect the prepared drug-loaded chitosan microspheres, respectively with petroleum ether, iso After washing with propanol and deionized water, the drug-loaded microspheres were prepared by freeze-drying after freezing at minus -10°C.

[0034] C. Preparation of coated alginic acid stent: Dissolve 1.3g of alginic acid in 100ml of deionized water to prepare an alginic acid solution, immerse the porous stent in step B in the above-mentioned alginic acid solution for 10 minutes and take it out at a speed of 2000r / min under centrifugation. Repeat 4 times, take o...

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Abstract

The invention provides a preparation method of a bone tissue engineering porous ceramics scaffold, belongs to the technical field of medical apparatus and instruments, and is capable of effectively solving the uniform distribution problem of drug carrying chitosan microspheres coating the surface of the bone tissue engineering porous ceramics scaffold. The preparation method comprises the steps of firstly preparing a porous scaffold, then preparing a drug carrying microspheres, and then preparing a ceramics scaffold coated with alginic acid. After obtaining the ceramics scaffold of which the surface is coated with the alginic acid, the drug carrying microspheres is needed to be combined with the ceramics scaffold: uniformly dispersing the prepared drug carrying microspheres into a centrifuge tube filled with deionized water, soaking the ceramics scaffold coated with the alginic acid into the centrifuge tube filled with the drug carrying microspheres, placing at the temperature of 37 DEG C, vibrating in a constant-temperature vibration box with the rotation speed of 60 to 120r/min for 1 to 3h, taking out the ceramics scaffold, repeatedly washing by using the deionized water, and then performing vacuum drying to obtain the bone tissue engineering porous ceramics scaffold which is coated with the alginic acid and provided with the uniformly-distributed drug carrying microspheres. The bone tissue engineering porous ceramics scaffold is mainly used for human bone tissue repair.

Description

technical field [0001] The invention belongs to the technical field of biomedical devices, and relates to the preparation technology of a bone tissue engineering bracket. Background technique [0002] As the main organ of the human skeletal system, bone is responsible for important functions such as movement, support and load bearing. However, bone defects caused by diseases, infections, trauma, aging, sports trauma and other reasons have become one of the clinical problems that need to be solved urgently. Although there are autologous bone transplantation, allogeneic bone transplantation or xenogeneic bone transplantation and other treatment methods in clinical application, there are problems such as insufficient source and immune row. At the same time, the development of bone tissue engineering technology brings new hope to solve this problem. [0003] In tissue engineering, porous three-dimensional scaffold materials play a key role, not only providing seed cells with a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/34A61L27/20A61L27/02
CPCA61L27/02A61L27/20A61L27/34A61L27/54A61L27/56A61L2300/21A61L2300/252A61L2300/412A61L2300/622A61L2430/02C08L5/04C08L5/08
Inventor 翁杰李金雨王琴许韬韬屈树新冯波
Owner SOUTHWEST JIAOTONG UNIV
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