Preparation method of immunomagnetic nanoparticles

A technology of magnetic nanoparticles and nanoparticles, which is applied in the field of preparation of immunomagnetic nanoparticles, can solve the problems of high technical requirements, cumbersome preparation process, difficult stable preparation, etc., and achieve simple and easy preparation process, good stability, and easy large-scale The effect of preparation

Active Publication Date: 2016-10-26
SHENZHEN HALDEX STC BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is to overcome technical bottlenecks such as high technical requirements of the prior art, cumbersome preparation process, involving organic toxic substances, difficulty in large-scale stable preparation, low coupling efficiency, poor stability, and easy shedding, so that Proposed a preparation process of immunomagnetic nanoparticles with high coupling efficiency, uniform particle size, good stability, simple process, short cycle, low toxicity, low cost, and no need for large-scale equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 This example discloses a method for preparing immunomagnetic nanoparticles (immunomagnetic nanoparticles coated with avidin / streptavidin), the method comprising the following steps:

[0035] (1) Preparation of ferromagnetic particle core of ferromagnetic tetroxide: weigh 40mmol FeCl 2 , dissolved in 4mL purified water, pour the mixed solution into 250ml containing 1mmol / L sodium citrate, 0.8mol / L sodium nitrate, 0.1mol / L sodium hydroxide, stir vigorously at 70°C for 60min, cool to room temperature, and use Wash twice with 1% TMAH, separate by magnetic force, and discard the supernatant. The precipitate was washed 2-3 times with 0.1% TMAH. Resuspend in 25 mL 0.1% TMAH.

[0036] (2) Surface silica-modified magnetic particles: take 25 mL of the magnetic particle inner core prepared in (1), add 3 ml of 10% ethanol solution of tetraethylorthosilicate, stir vigorously at room temperature for 3 hours, and wash twice with 0.1% TMAH , that is, the magnetic nanoparti...

Embodiment 2

[0039] Example 2 This example discloses a preparation method of immunomagnetic nanoparticles (immunomagnetic nanoparticles modified by rabbit anti-FITC antibody), the method comprising the following steps:

[0040] (1) Preparation of ferromagnetic ferromagnetic particle core: weigh 15mmol Fe2(SO 4 ) 3, dissolved in 4mL of purified water, pour the mixed solution into 100ml of 1% TMAH solution containing 0.5mmol / L sodium citrate and 1mol / L potassium nitrate, heat to 60°C, and vigorously stir for 30min. Cool to room temperature, magnetically separate, and discard the supernatant. The isolate was washed 2-3 times with 0.1% TMAH. Resuspend in 15 mL of 0.1% TMAH to obtain a stable magnetic suspension.

[0041] (2) Surface silica-modified magnetic particles: take 15 mL of the magnetic particle inner core prepared in (1), add 3 ml of 30% ethanol solution of tetraethoxyn-silane, stir vigorously at room temperature for 3 hours, wash with 1% TMAH for 2 times, the surface of the silic...

experiment example

[0045] Comparing the coupling efficiency and stability of the immune nanoparticles obtained in Example 1 and Example 2 with those obtained in the prior art, the following table is obtained:

[0046] Coupling efficiency

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PUM

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Abstract

The invention belongs to the field of biotechnologies, and particularly relates to a preparation method of immunomagnetic nanoparticles. The preparation method comprises the following steps: coating the surfaces of ferroferric oxide magnetic nanoparticles with silicate ester to lower the surface activation energy of the ferroferric oxide magnetic nanoparticles, so that the stability of the magnetic nanoparticles is enhanced greatly, and the concentration coating thickness of the silicate ester can be adjusted at the same time; then, modifying the surfaces of the magnetic nanoparticles with carboxyl, wherein in the whole modification process, reaction conditions are mild, an inert gas environment, severe conditions such as high temperatures and high pressures, complex devices and large-sized equipment are not needed, raw materials are readily available, a large quantity of organic solvents are not needed, and meanwhile the synthesized magnetic nanoparticles are adjustable in particle diameters; and activating the carboxyl-modified magnetic nanoparticles by an activating agent, and covalently binding the activated magnetic nanoparticles with free amino groups of bioactive molecules through amido bonds, wherein the covalent binding is firmer than non-covalent binding of an imine structure in an aldehyde-amine condensation reaction and colloidal gold.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of immunomagnetic nanoparticles. Background technique [0002] Ferroferric oxide nanoparticles have good biocompatibility and magnetic properties. Under the action of an external magnetic field, they can quickly aggregate and separate from other components, making them useful in cell sorting and labeling, protein separation and purification, and drug delivery. , Magnetic resonance imaging, cell labeling, targeted drug carriers, etc. have been widely used; however, ferric oxide nanoparticles without surface modification are in solution due to their small particle size, large surface activation energy, and interparticle static magnetism. It interacts with the galvanic moment, resulting in poor stability, easy agglomeration, deposition, and easy oxidation. Therefore, it needs to be modified to change its surface properties, prevent it from being oxidize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F1/36H01F41/02B01J13/02
CPCB01J13/02H01F1/344H01F1/36H01F41/02G01N33/531
Inventor 郑招荣刘金超
Owner SHENZHEN HALDEX STC BIOLOGICAL ENG CO LTD
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