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Enzyme substrate culture medium capable of simultaneously detecting two fecal pollution indicator bacteria and application thereof

A medium and enzyme substrate technology, applied in microorganisms, measuring devices, biological material analysis, etc., can solve the problems of cumbersome operation steps and long detection time, and achieve the effects of simple operation, short detection time and high sensitivity

Active Publication Date: 2016-11-09
中国疾病预防控制中心环境与健康相关产品安全所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In my country's environmental sanitation and food hygiene standards, there are limit requirements and inspection methods for fecal pollution indicator bacteria. The traditional detection methods for fecal pollution indicator bacteria are cumbersome and require confirmation tests to judge positive results. Long detection time

Method used

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  • Enzyme substrate culture medium capable of simultaneously detecting two fecal pollution indicator bacteria and application thereof
  • Enzyme substrate culture medium capable of simultaneously detecting two fecal pollution indicator bacteria and application thereof
  • Enzyme substrate culture medium capable of simultaneously detecting two fecal pollution indicator bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of liquid enzyme substrate culture medium capable of simultaneously detecting two kinds of feces pollution indicator bacteria

[0043] (1) Weigh each substance according to the following weights:

[0044]

[0045]

[0046] Wherein, the mixed trace elements contain: 55g of magnesium sulfate, 40g of calcium chloride, 2.5g of manganese sulfate, 2.4g of zinc sulfate, and 0.1g of copper sulfate.

[0047] (2) Dissolve the above-mentioned substances in distilled water, make up the volume to 1000L, and sterilize under high pressure.

Embodiment 2

[0048] Embodiment two can detect the preparation of the dry powder enzyme substrate culture medium of two kinds of fecal pollution indicator bacteria simultaneously

[0049] (1) Weigh each substance according to the following weights:

[0050]

[0051] Wherein, the mixed trace elements contain: 55g of magnesium sulfate, 40g of calcium chloride, 2.5g of manganese sulfate, 2.4g of zinc sulfate, and 0.1g of copper sulfate.

[0052] (2) Grind the above-mentioned substances into powder, mix well, make instant granules of 10-30 mesh at room temperature, subpackage, seal and sterilize according to the dose for testing 100mL water sample. Packed, each dose of dry powder enzyme substrate medium contains the following weights of each material:

[0053]

[0054]

[0055] Wherein, the mixed trace elements contain: 5.5 mg of magnesium sulfate, 4 mg of calcium chloride, 0.25 mg of manganese sulfate, 0.24 mg of zinc sulfate, and 0.01 mg of copper sulfate.

[0056] At room temperat...

Embodiment 3

[0057] Example 3 Qualitative detection of coliforms and Escherichia coli in water samples and comparison with conventional methods

[0058] Take 100 mL of untreated environmental water sample and place it in a sterile container with a cover of about 120 mL, add a dose of the above-mentioned dry powder enzyme substrate medium (prepared in Example 2), shake it evenly to dissolve it. Cover the container tightly and place it in an incubator at 36°C±1°C for 24h. If heat-resistant coliforms are detected, place the container in an environment of 44.5°C±1°C for 24 hours. Then observe the color change of the water sample in the container. If it is blue, it is positive for coliform or heat-resistant coliform. Observe under 366mn ultraviolet light, if there is fluorescence, it is Escherichia coli positive.

[0059] At the same time, the lactose fermentation method stipulated in the "Standard Test Methods for Drinking Water" (GB / T 5750.12-2006) was used to carry out the routine detectio...

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Abstract

The invention discloses an enzyme substrate culture medium capable of simultaneously detecting two fecal pollution indicator bacteria and application thereof. The culture medium contains ammonium sulfate, sodium chloride, disodium hydrogen phosphate, monopotassium phosphate, thiamine, folic acid, vitamin H, a mixed micro-element, a yeast extract, 5-bromine-4-chlorin-3-indol-beta-D-galactoside, 4-methyl umbelliferone-beta-D-glucuronide and isopropyl isopropylthiogalactoside. The culture medium can simultaneously detect coli groups and Escherichia coli in a sample on the culture condition of 44.5 DEG C+ / -1 DEG C. According to color changes and flurescent generation, existence of coli groups, heat resistance coli groups and Escherichia coli is determined. The detection means with easy and convenient operation and easily-judged results and without a confirmation test is provided for detection of fecal pollution indicator bacteria, and environment and food detection are promoted.

Description

technical field [0001] The invention relates to a culture medium and a method for simultaneously and rapidly detecting fecal contamination indicator bacteria in a sample, in particular to a culture medium and a method capable of simultaneously and rapidly detecting coliforms, thermotolerant coliforms or fecal coliforms in a sample A culture medium and method for Fecal Coliforms and Escherichia coli belong to the field of environmental microorganism detection. Background technique [0002] Diseases caused by microbial contamination seriously affect people's lives, and infectious diseases transmitted through the fecal-oral route seriously affect the quality of environmental hygiene and food hygiene. Fecal contamination indicator bacteria are the most important indicator bacteria in the field of environmental hygiene and food hygiene, including the large intestine. Colony (Coliforms), thermotolerant coliforms (Thermotolerant coliforms) or Fecal Coliforms (Fecal Coliforms) and E...

Claims

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/04C12R1/01
CPCC12Q1/045C12Q1/34G01N2333/938
Inventor 孙宗科丁培郑萍武利平李霞王友斌丁珵张晓毛怡心陈西平
Owner 中国疾病预防控制中心环境与健康相关产品安全所
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