Preparation method and application method of melanin precursor substance hair dye

A technology of melanin precursors and hair dyes, which is applied in the field of natural melanin precursors, can solve problems such as unstable performance, high cost, and long time consumption, and achieve safe, non-toxic, reliable and high-safety effects

Active Publication Date: 2019-05-17
珠海丝彩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide to hair dyes, and lack mature pigment extraction technology.
At present, there is a known method of using plant pigment catechol to prepare natural hair dye (patent document CN101164528A), but the catechol used in it is extracted from the Chinese medicinal material catechu, and its disadvantages are that the planting scale of the traditional Chinese medicinal material is limited and the cost of pigment extraction is limited. It is relatively high; it has also been reported that by genetic engineering technology, using the catechol oxidase expressed in the filamentous fungus cells of the genus Mycosis to oxidize dopa and its derivatives, and convert it into a method for melanin precursors (patent document CN101914585A ), but the mold itself will produce some toxic and harmful substances, and the production process will cause environmental pollution. At the same time, there are disadvantages such as high cost, long time consumption, unstable performance, and inability to apply in large quantities.
In the prior art, there is also a method of using the enzyme liquid extracted from the Bacillus fermentation liquid to catalyze the substrate to produce melanin precursors, but the safety and non-toxic side effects of the product cannot be guaranteed, and the pollution in the fermentation process will also affect the product quality.

Method used

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  • Preparation method and application method of melanin precursor substance hair dye
  • Preparation method and application method of melanin precursor substance hair dye
  • Preparation method and application method of melanin precursor substance hair dye

Examples

Experimental program
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Effect test

Embodiment 1

[0077] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0078] 1. Tyrosinase Extraction and Assay

[0079] Take 500 grams of crushed Pleurotus eryngii and add 1000 ml of distilled water to extract the tyrosinase solution or use 1000 ml of neutral phosphate buffer to extract the tyrosinase solution.

[0080] The measurement method of tyrosinase activity is as follows: Take 0.1ml of mushroom compound enzyme solution and 3.9ml of substrate solution to react at 317nm for 10min, and the enzyme activity unit U is defined as 0.01 units of absorbance change per 1min is 1 enzyme activity unit. When the measured result is greater than 1000U, the enzyme solution can be used for the following melanin precursor accumulation reaction.

[0081] Accumulation reactions of melanin precursors

[0082] Prepare 2L of tyrosine solution with a concentration of 1mM-10mM, add the tyrosine substrate solution into a bioreactor with ...

Embodiment 2

[0089] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0090] 1. Extraction of Tyrosinase

[0091] Use a juice extractor to crush the brown mushrooms, add 500g of the fruiting body residue to 1000ml, extract the enzyme solution with a citric acid buffer solution with a pH value of 6.8, use 5% activated carbon to absorb it, and then centrifuge it for later use.

[0092] Immobilization of tyrosinase

[0093] 200-mesh diatomite was heat-treated at 60°C for 30 minutes, soaked in phosphate buffer for 20 minutes, centrifuged to remove the supernatant, and the treated diatomite was added to the above enzyme solution for 5 hours to obtain immobilized enzyme for use .

[0094] Accumulation reactions of melanin precursors

[0095] Prepare 2L of tyrosine solution with a concentration of 5mM, which can be completely dissolved by heating and dissolving.

[0096] Add the tyrosine substrate solution into a bioreactor w...

Embodiment 3

[0103] The preparation method of the present embodiment melanin precursor is carried out according to the following steps:

[0104] 1. Extraction of Tyrosinase

[0105] Use a juicer to crush Agaricus bisporus, add 500g of crushed residue to 1000ml of Tris-hydrochloric acid buffer solution with a pH value of 7.0 to extract the enzyme liquid, extract the supernatant by centrifugation, and use 10% macroporous adsorption resin to absorb polysaccharides and miscellaneous proteins in the enzyme liquid. The treated enzyme solution was stored at 4°C for later use.

[0106] Accumulation reactions of melanin precursors

[0107] Prepare 2L of tyrosine solution with a concentration of 10mM, which can be completely dissolved by heating and dissolving.

[0108] Reaction process is with embodiment 1.

[0109] The liquid sample taken out was filtered through filter paper, and after the filtrate was diluted 10 times, the absorbance at 300 nm and 475 nm was measured in an ultraviolet spectro...

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Abstract

The invention discloses a melanin precursor substance hair dye. The melanin precursor substance hair dye consists of a melanin precursor, a common hair dye adjuvant and water, wherein the melanin precursor includes dopachrome, 5,6-dihydroxyindole and 5,6-dihydroxyindole-2-carboxylic acid; the melanin precursor accounts for 0.01-5% of the hair dye in percentage by weight; and the common hair dye adjuvant includes one or more of a wetting penetrating agent, a thickening agent, a surfactant, a moisturizer and a pH value regulating agent. The hair dye disclosed by the invention combines the advantages of a chemically synthetic hair dye and a natural hair dye; by extracting complex enzyme liquid in natural edible fungi, such melanin precursor substances as the dopachrome, the 5,6-dihydroxyindole, the 5,6-dihydroxyindole-2-carboxylic acid and the like are synthesized through bio-conversion by virtue of amino acid, so that the hair dye is free from irritation and allergy to skin, high in safety and capable of keeping a natural black hair dyeing effect; and the hair dye has huge popularization and application values in the field of the preparation of hair dyes.

Description

technical field [0001] The invention relates to a hair dye prepared by enzyme treatment, in particular to a compound enzyme solution for extracting edible fungus enzymes, converting an amino acid substrate into a natural melanin precursor substance, and preparing it into a non-oxidative biological hair dye method. Background technique [0002] At present, with the continuous improvement of people's attention to health, chemically synthesized hair dyes contain carcinogenic and strong allergenic chemical dyeing ingredients (such as phenylenediamines, aminophenols, etc.) and some harmful ingredients (such as hydrogen peroxide) , ammonia, etc.), has been more and more people's attention. For this reason, people try to seek natural harmless, safer hair dyeing materials. For example, plant pigment hair dyes have achieved certain results, but there are still many problems. Such products still cannot avoid adding harmful substances such as phenols and hydrogen peroxide in hair dye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/49A61K8/44A61K8/41C12P17/10A61Q5/10
CPCA61K8/41A61K8/44A61K8/492A61K2800/4324A61Q5/10C12P17/10
Inventor 张然薛俊厉秀茹
Owner 珠海丝彩生物科技有限公司
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